Supplementary Table S1 from Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression

Sequences of each primer for real-time quantitative PCR</p

Figure S2 from Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression

Scratch of a gap for wound healing assay was generated and closure of the gap was evaluated 24 hours later in FTC236-Notch1 cells with or without doxycycline treatment. The cells with NICD induction by doxycycline exhibited slower closure of the gap than those without NICD induction. Images were taken immediately after scratching the cultures 0 hr and 24hr later. Representative images at 0 hr and 24 hr are shown in the upper panel. The bar graph represents the average percentage of the gap closure. Each bar represents mean{plus minus} SEM; "Dox" indicates doxycycline; ** indicates p< 0.01; Scale bar:200µm.</p

Figure S4 from Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression

SERPINE1 expression detected by immunohistochemistry in FTC236-Notch1 xenografts with or without Notch1 induction. (A) and (B). Two representative tumors from the group without Notch1 induction (Dox-); (C) and (D). Two representative tumors from the group with Notch1 induction (Dox+). Weaker staining of SERPINE1 (1+) is shown in the Dox+ tumors compared with 3+ staining in Dox- tumors. "Dox" indicates doxycycline; Scale bar: 50µm.</p

Figure S1 from Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression

The correlation between age and expression levels of Notch1 in primary DTC samples (A), contra-lateral non-tumor tissues (B) and tissue of benign thyroid disease (C).(A) Scatter plot of negative linear correlation between Notch1 levels and age in primary DTC samples (R = -0.495). (B)-(C) Significant linear correlation was not observed between age and Notch1 levels in the non-tumor tissues or benign thyroid lesions. Pearson's correlation test was used to compute the correlation significance between the 2 variables.</p

Figure legends (supplementary figures) from Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression

Figure legends for Figure S1-S4</p

Material and Methods (supplementary) from Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression

Described details of Western blot, clonogenic assay, cell migration assay, ex-vivo imaging, IHC and etc.</p

Figure S3 from Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression

RFP-positive FTC236-Notch1 cells for the establishment of orthotopic mouse model. (A). Over 26% of the cells from the initial transduced batch were positive for RFP and were sorted for further culturing. (B). The sorted cells were cultured for 3 passages and over 96% were RFP-positive. (C). The sorted cells were cultured for 6 passages and 97% were RFP-positive.</p

Supplementary Table S2 from Notch1 Signaling Regulates the Aggressiveness of Differentiated Thyroid Cancer and Inhibits SERPINE1 Expression

The top 50 most significant hits from cDNA microarray analysis</p

DataSheet_1_Ex Vivo Modeling of Human Neuroendocrine Tumors in Tissue Surrogates.docx

Few models exist for studying neuroendocrine tumors (NETs), and there are mounting concerns that the currently available array of cell lines is not representative of NET biology. The lack of stable patient-derived NET xenograft models further limits the scientific community’s ability to make conclusions about NETs and their response to therapy in patients. To address these limitations, we propose the use of an ex vivo 3D flow-perfusion bioreactor system for culturing and studying patient-derived NET surrogates. Herein, we demonstrate the utility of the bioreactor system for culturing NET surrogates and provide methods for evaluating the efficacy of therapeutic agents on human NET cell line xenograft constructs and patient-derived NET surrogates. We also demonstrate that patient-derived NET tissues can be propagated using the bioreactor system and investigate the near-infrared (NIR) dye IR-783 for its use in monitoring their status within the bioreactor. The results indicate that the bioreactor system and similar 3D culture models may be valuable tools for culturing patient-derived NETs and monitoring their response to therapy ex vivo.</p