Haplotype diversity, nucleotide diversity of different ploidy populations of <i>S</i>. <i>spontaneum</i> according to rDNA-ITS haplotype data.

Haplotype diversity, nucleotide diversity of different ploidy populations of S. spontaneum according to rDNA-ITS haplotype data.</p

Molecular variance (AMOVA) analysis among different ploidy populations according to rDNA-ITS haplotype data.

<p>Molecular variance (AMOVA) analysis among different ploidy populations according to rDNA-ITS haplotype data.</p

The list of control rDNA-ITS sequences.

<p>The list of control rDNA-ITS sequences.</p

The GC content analysis of composition of ITS sequence of different ploidy populations of <i>S</i>. <i>spontaneum</i>.

<p>The GC content analysis of composition of ITS sequence of different ploidy populations of <i>S</i>. <i>spontaneum</i>.</p

Molecular variance (AMOVA) analysis among different ploidy populations according to rDNA-ITS haplotype data.

Molecular variance (AMOVA) analysis among different ploidy populations according to rDNA-ITS haplotype data.</p

The list of clones of <i>S</i>. <i>spontaneum</i> used in this study.

<p>The list of clones of <i>S</i>. <i>spontaneum</i> used in this study.</p

The analysis of variable sites of ITS sequence of different ploidy populations of <i>S</i>. <i>spontaneum</i>.

<p>The analysis of variable sites of ITS sequence of different ploidy populations of <i>S</i>. <i>spontaneum</i>.</p

Pairwise Gst (above the diagonal) and Nm (below the diagonal) among different ploidy populations according to rDNA-ITS data.

<p>Pairwise Gst (above the diagonal) and Nm (below the diagonal) among different ploidy populations according to rDNA-ITS data.</p

The ML and NJ phylogenetic tree based on rDNA-ITS haplotype data of different polyploid clones of <i>S</i>. <i>spontaneum</i>.

<p>The ML and NJ phylogenetic tree based on rDNA-ITS haplotype data of different polyploid clones of <i>S</i>. <i>spontaneum</i>.</p

Additional file 3: of An improved suppression subtractive hybridization technique to develop species-specific repetitive sequences from Erianthus arundinaceus (Saccharum complex)

Figure S3. Electrophoresis of secondary PCR products after SSH. M: 100 bp marker; Lanes 1–4: products of subtracted second PCR by 62 °C, 64 °C, 66 °C, 68 °C. 20 μM primers and 0.5 U LA Taq polymerase (a), 20 μM primers and 1 U LA Taq polymerase (b), 30 μM primers and 0.5 U LA Taq polymerase (c). (TIF 11035 kb