Psychologic evaluation of patients undergoing dorsal sympathicotomy for the treatment of bilateral palmar and axillary hyperhidrosis [Valutazione psicologica in pazienti sottoposti a simpaticotomia selettiva per iperidrosi palmare ed ascellare bilaterale]

Additional file 1: Table S1. Yeast strains used in this study. Table S2. List of primers used for RT-qPCR analysis in this work. Table S3. List of primers used for plasmids and strains construction in this work. Fig. S1. Influence of acetic acid stress on transcription of ADE17 in different S. cerevisiae BY4741 strains. Fig. S2. Comparison of stress tolerance of the engineered yeast strains with that of the control strain BHO under various stressful conditions. Fig. S3. Potential transcription factors regulating the ADE genes. Fig. S4. Growth curve of the recombinant strains under various conditions. Fig. S5. Detoxification of furfural and 5-HMF by the recombinant yeast strains. Fig. S6. Effect of ADE genes overexpression on intracellular energy level at stationary phase. Fig. S7. Effect of amino acids addition on yeast growth under acetic acid stress condition. Fig. S8. Comparison of acetic acid tolerance of the mutant strain ADE17_mZRE and the control strain. Fig. S9. Impact of the ADE genes overexpression on succinic acid production

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Additional file 2: Table S2. Composition of total raw reads and statistical results of samples mapped to gene and genome

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Additional file 4: Table S4. Differentially expressed proteins between “P0” and “F3”

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Additional file 5: Table S5. Model summaries for the discrimination between the Pi-replete and Pi-limited samples from LC-MS data for metabolomic analysis

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Additional file 1: Table S1. Primers used in this study

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Additional file 3: Table S3. Differentially expressed genes between “P0” and “F3”

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Additional file 7: Figure S1. Comparative metabolomic analyses of R. toruloides samples prepared under Pi-limited (P0) and Pi-replete (F3) conditions. a Total ion chromatogram of quality control (QC) sample (positive). b Total ion chromatogram of QC sample (negative). c Changes of some cellular nucleoside derivatives and bases. Figure S2. Metabolomic analysis of R. toruloides during phosphate-limitation. a Score plot of PCA in P0 vs F3 (positive). b Score plot of PLS-DA in P0 vs F3 (positive). c Sorting plot of PLS-DA in P0 vs F3 (positive). d Score plot of OPLS-DA in P0 vs F3 (positive). Figure S3. Metabolomic analysis of R. toruloides during phosphate-limitation. a Score plot of PCA in P0 vs F3 (negative). b Score plot of PLS-DA in P0 vs F3 (negative). c Sorting plot of PLS-DA in P0 vs F3 (negative). d Score plot of OPLS-DA in P0 vs F3 (negative)