Synthesis of Double-Chain Bis-Sulfone Neoglycolipids of the 2-, 3-, and 6-Deoxyglobotrioses

Partially protected 2-(trimethylsilyl)ethyl 2- and 3-deoxyglucosides and 6-deoxylactoside were synthesised via various routes and glycosylated with galabiosyl and galactosyl donors to give the corresponding deoxytrisaccharides. Removal of the protecting groups gave the 2-(trimethylsilyl)ethyl 2-, 3-, and 6-deoxyglobotriosides. Transformation of the protected trisaccharides into trichloroacetimidates, via the corresponding hemiacetals, proceeded in ∼80% overall yield. Glycosylation of 3-(hexadecylsulfonyl)-2-[(hexadecylsulfonyl)methyl]propanol with the trisaccharidic trichloroacetimidates, in 72−79% yield, followed by removal of protecting groups, gave the title neoglycolipids

DDQ-Mediated Oxidation of 4,6-<i>O</i>-Methoxybenzylidene-Protected Saccharides in the Presence of Various Nucleophiles: Formation of 4-OH, 6-Cl, and 6-Br Derivatives

Treatment of 4,6-O-p-methoxybenzylidene-protected pyranosidic mono- and disaccharides with 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ), in the presence of a few equivalents of water, gave the corresponding 6- and 4-O-p-methoxybenzoates with unprotected hydroxyl groups in the 4- and 6-position in the ratio ∼4:1 and in 85−98% yield. Dry conditions in the presence of halide salts gave the 6-deoxychloro and -bromo 4-O-p-methoxybenzoates exclusively, in >90% yield

Synthesis of Double-Chain Bis-Sulfone Neoglycolipids of the 2-, 3-, and 6-Deoxyglobotrioses

Partially protected 2-(trimethylsilyl)ethyl 2- and 3-deoxyglucosides and 6-deoxylactoside were synthesised via various routes and glycosylated with galabiosyl and galactosyl donors to give the corresponding deoxytrisaccharides. Removal of the protecting groups gave the 2-(trimethylsilyl)ethyl 2-, 3-, and 6-deoxyglobotriosides. Transformation of the protected trisaccharides into trichloroacetimidates, via the corresponding hemiacetals, proceeded in ∼80% overall yield. Glycosylation of 3-(hexadecylsulfonyl)-2-[(hexadecylsulfonyl)methyl]propanol with the trisaccharidic trichloroacetimidates, in 72−79% yield, followed by removal of protecting groups, gave the title neoglycolipids

Additional file 1 of Adjuvant music therapy for patients with hypertension: a meta-analysis and systematic review

Additional file 1: Supplementary 1. PubMed search strategy tabl

Assembly of Oligosaccharide Libraries with a Designed Building Block and an Efficient Orthogonal Protection−Deprotection Strategy

Assembly of Oligosaccharide Libraries with a Designed Building Block and an Efficient Orthogonal Protection−Deprotection Strateg

Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma-0

Rs in each experimental group is shown at the end of treatment (L = left side, R = right side). Both As-ODN and ATRA treatment resulted in an inhibition of tumor volume and tumor weight as compared to control group. * < 0.01, one-way ANOVA. In addition, highly significant interactions exist between hTR As-ODN and ATRA. * * F = 10.743, = 0.002, two-way ANOVA. The combination of As-ODN and ATRA resulted in a significant enhancement of the reduction in tumor growth when compared with monotherapy of As-ODN or ATRA alone (< 0.01).<p><b>Copyright information:</b></p><p>Taken from "Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/8/159</p><p>BMC Cancer 2008;8():159-159.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2427037.</p><p></p

Additional file 3 of SYT7 acts as an oncogene and a potential therapeutic target and was regulated by ΔNp63α in HNSCC

Additional file 3: Figure S1. Establishment of ΔNP63α-overexpressed cells. A–D The fluorescence observation of ΔNP63α-overexpressed HN6 cells. E–H The fluorescence observation of ΔNP63α-overexpressed CAL-27 cells. I The expression levels of ΔNP63α mRNA in ΔNP63α-overexpressed HN6 cells. J The expression levels of ΔNP63α mRNA in ΔNP63α-overexpressed CAL-27 cells. K The expression levels of ΔNP63α protein in ΔNP63α-overexpressed HN6 cells. L The expression levels of ΔNP63α protein in ΔNP63α-overexpressed CAL-27 cells (n = 3). Bars show the mean ± SD. *Represents p < 0.05, **represents p < 0.01. Scale bar = 100 μm

Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma-2

-ODN group and control group compared with other treatment groups. * < 0.01, one-way ANOVA. A significant interaction was observed between As-ODN-hTR and ATRA treatment. * * F = 4.507, = 0.041, two-way ANOVA.<p><b>Copyright information:</b></p><p>Taken from "Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/8/159</p><p>BMC Cancer 2008;8():159-159.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2427037.</p><p></p

Additional file 2 of SYT7 acts as an oncogene and a potential therapeutic target and was regulated by ΔNp63α in HNSCC

Additional file 2: Table S2. Antibodies used in westernblotting and IHC

Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma-3

Asmic staining in (C) As group, (D) ATRA group and (E) S/ATRA group, extremely weak Bcl-2 staining in (F) As/ATRA treated group. (G) Similar moderate Bax cytoplasmic staining of tumor cells in all treatment groups. Representative section of As/ATRA group is shown. Scale bar = 50 μm. Computer-assisted quantitation of immunohistochemical staining was performed. (H) The average IODs of Bcl-2 and Bax were shown as the mean ± SE (n = 10). The average IODs in As-ODN and ATRA treated groups were significantly decreased as compared to S-ODN group and control group. * < 0.01, one-way ANOVA. Significant interaction was observed between As-ODN-hTR and ATRA treatment. * * F = 35.836, < 0.01, two-way ANOVA. There was no significant difference of Bax expression in all treatment groups.<p><b>Copyright information:</b></p><p>Taken from "Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/8/159</p><p>BMC Cancer 2008;8():159-159.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2427037.</p><p></p