Overexpressed miRNAs in TB sputum.

<p>Column “Name” contained the name of miRNA; Column “TB patients versus control” contained level ratio of TB/control; Column “Chromosome” meant distribution of each miRNA on chromosome.</p><p>Each miRNA spot was replicated for four times on the same slide and two microarray chips were used for each group. After normalization, obtained average values for each miRNA spot were used for statistics. The <i>P</i> values for these miRNAs were less than 0.05 in TB group compared with controls.</p

Oligonucleotides used in this study.

<p>Oligonucleotides used in this study.</p

Confirmation miRNA level by RT-qPCR.

<p>RT-qPCR analysis confirmed microarray data. After normalization to U6 RNA, data were presented as mean ± SD (n = 30) and obtained average value for each miRNA was used for statistics. MiR-19b-2* was underexpressed while miR-3179 and miR-147 were overexpressed in TB sputum compared with controls. The experiment was conducted in triplicate. ^#<i>P</i><0.05 versus control.</p

Hierarchical clustering of miRNA in sputum samples.

<p>Samples were clustered according to the signature profile of 97 differentially expressed miRNAs. Data from each miRNA were median centered. Samples were in columns and miRNAs in rows. Red and green indicated high relative level and low relative level, respectively. The <i>P</i> values for these miRNAs were less than 0.05 in TB group compared with controls.</p

Characteristics of participants.

<p>All patients had clinical signs and symptoms of active pulmonary TB; comprising, 79.3% cough, 72.4% fever, 67.2% weight loss, 55.2% night sweats, and 46.6% hemoptysis. Healthy controls involved in the study, were free of active TB infection, latent TB infection and any clinical symptoms of any infectious disease. Both TB patients and healthy controls were non-smokers.</p

Underexpressed miRNAs in TB sputum.

<p>Column “Name” contained the name of miRNA; Column “TB patients versus control” contained level ratio of TB/control; Column “Chromosome” meant distribution of each miRNA on chromosome.</p><p>Each miRNA spot was replicated for four times on the same slide and two microarray chips were used for each group. After normalization, obtained average values for each miRNA spot were used for statistics. The <i>P</i> values for these miRNAs were less than 0.05 in TB group compared with controls.</p