PLAGL2 expression and clinicopathological variables in 79 PCa patients.

PLAGL2 expression and clinicopathological variables in 79 PCa patients.</p

Association between PLAGL2 expression and BCR-free survival and OS of PCa patients assessed by Kaplan–Meier survival curves.

<p>(A) The patients with high expression had significantly shorter median 5-year BCR-free survival than those with low PLAGL2 expression. (B) The patients with high expression had significantly shorter median 5-year OS than those with low PLAGL2 expression.</p

Expression of PLAGL2 in paired PCa with adjacent normal prostate tissues and cell lines revealed by qRT-PCR and western blot.

<p>(A) Levels of PLAGL2 mRNA in 25 PCa tissues were significantly higher than those in adjacent noncancerous tissues measured by qRT-PCR (P<0.0001). (B) The PLAGL2 protein level in PCa tissues was upregulated in comparison with adjacent noncancerous tissues measured by Western blot. (C) Western blot indicated the PLAGL2 protein showed higher expression in PCa tissues than in their adjacent normal counterparts. (D) The expression of PLAGL2 mRNA was higher in PCa cell lines (LNCaP, DU145 and PC3) than in RWPE-1 (P<0.0001). (E) Western blot indicated up-regulation of PLAGL2 protein in PCa cell lines (LNCaP, DU145 and PC3) in comparison with RWPE-1.</p

Immunohistochemical staining for PLAGL2 in PCa tissue and BPH tissue.

<p>The PLAGL2 expression was predominantly localized in nuclei (positive staining indicated by arrows). (A) BPH tissue (low staining). (B) PCa tissue (low Gleason Score). (C) PCa tissue (high Gleason Score). ×400 magnification.</p

Prognostic value of PLAGL2 expression for the OS revealed by univariate and multivariate analyses.

<p>Prognostic value of PLAGL2 expression for the OS revealed by univariate and multivariate analyses.</p

Prognostic value of PLAGL2 expression for the BCR-free survival revealed by univariate and multivariate analyses.

<p>Prognostic value of PLAGL2 expression for the BCR-free survival revealed by univariate and multivariate analyses.</p

Expression of PLAGL2 in prostate specimens.

<p>Expression of PLAGL2 in prostate specimens.</p

Analysis on PLAGL2 expression in PCa and normal prostate tissues with microarray datasets of PCa and log2 copy number unites of DNA or log2 median intensity mRNA.

<p>(A, B) The PLAGL2 DNA copy number and mRNA level was significantly higher in PCa tissues than non-tumor prostate tissues. (C) The PLAGL2 mRNA level was significantly higher in metastatic PCa compared to primary PCa patients. (D) The PLAGL2 mRNA level was significantly increased in higher Gleason score compared with lower Gleason score. (E) The PLAGL2 DNA copy number was significantly higher in earlier recurrence of PCa patients.</p

Effect of ozone oxidative preconditioning on inflammation and oxidative stress injury in rat model of renal transplantation

<div><p>Abstract Purpose: To investigate the effect of ozone oxidative preconditioning (OzoneOP) on inflammation and oxidative stress injury in rat model of renal transplantation. Methods: Thirty six male Sprague Dawley (SD) rats were randomly divided into three groups. Sham group: rats were treated with opening and closing abdomen. Kidney transplantation group (KT group): SD rat received the donor’s left kidney derived from another SD rat. Ozone oxidative preconditioning and kidney transplantation (OOP+KT group): donor SD rats received OzoneOP treatments by transrectal insufflations before kidney transplantation. After transplantation, parameters of renal function of recipients were determined. Morphology and pathological changes of renal allograft were examined. Expression of NF-κBp65, HMGB-1 were also determined by Western-blot. Results: Compared to KT group, the morphology and pathological damages of renal allograft were less serious in OOP+KT group. Meanwhile, levels of SOD and GSH-Px of renal allograft in OOP+KT group were higher than those in KT group respectively. Western-blot showed that the expressions of NF-κBp65 and HMGB-1 in OOP+KT group were obviously less than those in KT group. Conclusion: Ozone oxidative preconditioning could attenuate the inflammatory reaction and oxidative stress injury in renal allograft, which might be related with the enhancement of anti-oxidative system and suppression of inflammatory reaction.</p></div

Resistive Switching Induced by Metallic Filaments Formation through Poly(3,4-ethylene-dioxythiophene):Poly(styrenesulfonate)

We report the design and fabrication of Al/poly­(3,4-ethylene-dioxythiophene):poly­(styrenesulfonate) (PEDOT:PSS)/Cu resistive memory devices that utilize the Cu redox reaction and conformational features of PEDOT:PSS to achieve resistive switching. The top Cu electrode acts as the source of the redox ions that are injected through the PEDOT:PSS layer during the forming process. The Cu filament is confirmed directly using the cross-sectional images of transmission electron microscopy and energy-dispersive X-ray spectroscopy. The resultant resistive memory devices can operate over a small voltage range, i.e., the switching-on threshold voltage is less than 1.5 V and the absolute value of the switching-off threshold voltage is less than 1.0 V. The on/off current ratio is as large as 1 × 10⁴ and the two different resistance states can be maintained over 10⁶ s. Moreover, the devices present good thermal stability that the resistive switching can be observed even at temperature up to 160 °C, at which the oxidation of the Cu top electrode is the failure factor. Furthermore, the cause of failure for Al/PEDOT:PSS/Cu memory devices at higher temperature is confirmed to be the oxidation of Cu top electrode