Related Article from The Role of Protein Kinase CK2 in Glioblastoma Development

Related Article from The Role of Protein Kinase CK2 in Glioblastoma Developmen

Additional file 1 of A pan-cancer analysis of the oncogenic role of ERCC6L

Additional file 1. Supplementary figures

Study on the Deactivation of V₂O₅–WO₃/TiO₂ Selective Catalytic Reduction Catalysts through Transient Kinetics

The mechanism underlying the deactivation of a commercial V₂O₅–WO₃/TiO₂ catalyst for NH₃ selective catalytic reduction (SCR) of NO_<i>x</i> through exposure to the flue gas of a coal-fired power plant was investigated by a transient kinetic analysis that focused on the distinction between the deactivation behaviors of adsorption sites and redox sites. The results showed that alkali dopants preferentially poison the active sites associated with vanadium (V⁵⁺–OH and/or V⁵⁺O) rather than the sites associated with titania and tungsten. Obvious changes in the activation energies for NH₃ desorption, oxidation, and SCR surface reaction over the used catalyst were observed. Kinetic variations showed that three other factors that are involved in the elementary surface steps are responsible for the catalyst deactivation rather than simply the decline of the NH₃ adsorption capacity. Finally, the effects of these factors on the catalyst activity were analyzed at different temperatures

Altered peripheral lymphocyte subsets in untreated systemic lupus erythematosus patients with infections

The leading cause of death in systemic lupus erythematosus (SLE) patients is infection. The objective of this study was to evaluate the distribution of lymphocyte subsets in untreated SLE patients with infections. This was a cross-sectional study. Data from January 2017 to May 2018 were collected. Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B cells, CD3-CD16+CD56NK cells, and CD3+CD16+CD56NKT cells in 25 healthy controls and 52 treatment-naive SLE patients, among whom 13 were complicated with infections. Association between the lymphocyte subsets and infections was further analyzed. SLE patients with infections (n=13) showed a significantly higher incidence rate of fever (84.6 vs 28.2%) and serositis (84.6 vs 23.1%), increased level of erythrocyte sedimentation rate (60.5±30.1 vs 37.4±27.1 mm/h), serum C-reactive protein (CRP) (102.7±94.9 vs 9.4±14.9 mg/L), procalcitonin (PCT) (1.07±0.08 vs 0.16±0.13 μg/L), and lower blood hemoglobin (Hb) (93.0±20.5 vs 110.4±16.0 g/L) level compared with non-infection patients (n=39) (all P</div

Supplemental Material - Exploiting Data Uncertainty for Improving the Performance of a Quantitative Analysis Model for Laser-Induced Breakdown Spectroscopy

Supplemental Material for Exploiting Data Uncertainty for Improving the Performance of a Quantitative Analysis Model for Laser-Induced Breakdown Spectroscopy by Huaiqing Qin, Ziyu Yu, Zhimin Lu, Zhuliang Yu, and Shunchun Yao in Applied Spectroscopy</p

Data_Sheet_1_Differential Variation in Non-structural Carbohydrates in Root Branch Orders of Fraxinus mandshurica Rupr. Seedlings Across Different Drought Intensities and Soil Substrates.docx

Non-structural carbohydrates (NSCs) facilitate plant adaptation to drought stress, characterize tree growth and survival ability, and buffer against external disturbances. Previous studies have focused on the distribution and dynamics of NSCs among different plant organs under drought conditions. However, discussion about the NSC levels of fine roots in different root branch orders is limited, especially the relationship between fine root trait variation and NSC content. The objective of the study was to shed light on the synergistic variation in fine root traits and NSC content in different root branch orders under different drought and soil substrate conditions. The 2-year-old Fraxinus mandshurica Rupr. potted seedlings were planted in three different soil substrates (humus, loam, and sandy–loam soil) and subjected to four drought intensities (CK, mild drought, moderate drought, and severe drought) for 2 months. With increasing drought intensity, the biomass of fine roots decreased significantly. Under the same drought intensity, seedlings in sandy–loam soil had higher root biomass, and the coefficient of variation of 5th-order roots (37.4, 44.5, and 53% in humus, loam, and sandy–loam soil, respectively) was higher than that of lower-order roots. All branch order roots of seedlings in humus soil had the largest specific root length (SRL) and specific root surface area (SRA), in addition to the lowest diameter. With increasing drought intensity, the SRL and average diameter (AD) of all root branch orders increased and decreased, respectively. The fine roots in humus soil had a higher soluble sugar (SS) content and lower starch (ST) content compared to the loam and sandy–loam soil. Additionally, the SS and ST contents of fine roots showed decreasing and increasing tendencies with increasing drought intensities, respectively. SS and ST explained the highest degree of the total variation in fine root traits, which were 32 and 32.1%, respectively. With increasing root order, the explanation of the variation in root traits by ST decreased (only 6.8% for 5th-order roots). The observed response in terms of morphological traits of different fine root branch orders of F. mandshurica seedlings to resource fluctuations ensures the maintenance of a low cost-benefit ratio in the root system development.</p

DataSheet_1_Iguratimod Restrains Circulating Follicular Helper T Cell Function by Inhibiting Glucose Metabolism via Hif1α-HK2 Axis in Rheumatoid Arthritis.docx

Iguratimod (IGU) is a novel disease modified anti-rheumatic drug, which has been found to act directly on B cells for inhibiting the production of antibodies in rheumatoid arthritis (RA) patients. Follicular helper T (Tfh) cells, a key T cell subsets in supporting B cell differentiation and antibody production, have been shown to play critical roles in RA. However, whether IGU can inhibit RA Tfh cells which further restrains B cell function remains unclear. Here, we aimed to explore the roles of IGU in regulating RA circulating Tfh (cTfh) cell function and investigate the potential mechanism associated with cell glucose metabolism. In our study, we found that IGU could act on RA-CD4+ T cells to reduce T cell-dependent antibody production. IGU decreased the percentage of RA cTfh cells and the expression of Tfh cell-related molecules and cytokines which were involved in B cell functions. Importantly, our data showed that IGU significantly restrained the cTfh cell function by inhibiting glucose metabolism, which relied on Hif1α-HK2 axis. In summary, we clarified a new target and mechanism of IGU by restraining RA cTfh cell function via inhibiting Hif1α-HK2-glucose metabolism axis. Our study demonstrates the potential application of IGU in the treatment of diseases related to abnormal metabolism and function of Tfh cells.</p

Additional file 1 of Increased oxidative stress contributes to impaired peripheral CD56dimCD57+ NK cells from patients with systemic lupus erythematosus

Additional file 1: Supplementary Table 1. Antibody list. Supplementary Table 2. Baseline characteristics of patients and healthy controls in this study. Supplementary Table 3. Hallmark gene sets((Lupus vs Control)*. Supplementary Table 4. Hallmark gene sets((CD57+ vs CD57-)*. Supplementary Figure 1. The NK cell subset gating strategies. Supplementary Figure 2. Supplementary analysis of clinical data and peripheral NK count in SLE and RA patients. Supplementary Figure 3. The correlation of CD56dimCD57+ NK cell percentage with SLEDAI (n=37). Supplementary Figure 4. Apoptosis and ROS levels of sorted NK cells upon exposure to H2O2. Supplementary Figure 5. Cytokine expression of CD56dimCD57+ NK cells in SLE patients and HCs. Supplementary Figure 6. No cytotoxicity of resting CD4+ T cells by NK cells(n=3). Supplementary Figure 7. PD-1 expression of NK cell subsets in SLE patients (n=8) and HCs (n=8)

Figure S3 from Conversion of PRPS Hexamer to Monomer by AMPK-Mediated Phosphorylation Inhibits Nucleotide Synthesis in Response to Energy Stress

AMPK-dependent PRPS1/2 phosphorylation converts PRPS1/2 hexamers to monomers.</p

Figure S2 from Conversion of PRPS Hexamer to Monomer by AMPK-Mediated Phosphorylation Inhibits Nucleotide Synthesis in Response to Energy Stress

AMPK phosphorylates PRPS1 S180 and PRPS2 S183 in response to glucose deprivation.</p