Nachrichten aus der Brüder-Gemeine, 1828, no. 05

The Moravian Church's monthly journal of its worldwide activities, including in Labrador, published from 1819-94

Single Amino Acid Variant Profiles of Subpopulations in the MCF‑7 Breast Cancer Cell Line

Cancers are initiated and developed from a small population of stem-like cells termed cancer stem cells (CSCs). There is heterogeneity among this CSC population that leads to multiple subpopulations with their own distinct biological features and protein expression. The protein expression and function may be impacted by amino acid variants that can occur largely due to single nucleotide changes. We have thus performed proteomic analysis of breast CSC subpopulations by mass spectrometry to study the presence of single amino acid variants (SAAVs) and their relation to breast cancer. We have used CSC markers to isolate pure breast CSC subpopulation fractions (ALDH+ and CD44+/CD24– cell populations) and the mature luminal cells (CD49f–EpCAM+) from the MCF-7 breast cancer cell line. By searching the Swiss-CanSAAVs database, 374 unique SAAVs were identified in total, where 27 are cancer-related SAAVs. 135 unique SAAVs were found in the CSC population compared with the mature luminal cells. The distribution of SAAVs detected in MCF-7 cells was compared with those predicted from the Swiss-CanSAAVs database, where we found distinct differences in the numbers of SAAVs detected relative to that expected from the Swiss-CanSAAVs database for several of the amino acids

Representative images of CD90 (green) with CD45(red) both in PanIN III and PanIN III.

<p>Nuclei visualization was shown by staining DAPI (blue). CD45 was negative in PanINs. Scale bars = 100 μm.</p

Co-expression of CD90 (green) and CD24(red) in PanIN.

<p>Nuclei visualization was shown by staining DAPI (blue). CD90 mainly presented in stromal cells, however, CD24 expressed in cytoplasm and membrane of pancreatic ductal epithelium. There was little overlap in all stages. Scale bars = 100 μm.</p

Expression of CD24 (red) in normal and various PanIN tissues.

<p>Nuclei visualization was shown by staining DAPI (blue). CD24 presented weak or negative expression in acinar cells, and no staining was observed in pancreatic ductal epithelium in the normal goup. CD24 was mainly present in the cytoplasm and membrane of the pancreatic ductal epithelium, especially in the apical epithelium of the duct (arrow). Scale bars = 100 μm.</p

Three PanIN hispathological grades (HE).

<p>A:normal pancreas; B:PanIN III, minimal cytological atypia (arrow); C:PanIN III, nuclear polarity disappears, moderate cytological atypia (arrow); D: PanIN III, nuclear atypia hyperplasia, papillary or micro papillary morphology (arrow). Scale bars = 100 μm.</p