59 research outputs found
Hydrothermal synthesis of fluorescent nitrogen-doped carbon quantum dots from ascorbic acid and valine for selective determination of picric acid in water samples
<p>Nitrogen doped carbon quantum dots (N-CQDs) were synthesised by a hydrothermal method using ascorbic acid and valine as precursors. The as-synthesised N-CQDs were characterised by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, UV−vis absorption spectra, as well as fluorescence spectrophotometer. The results revealed that the as-prepared N-CQDs were spherical shaped with an average diameter of 4 nm and emitted bright blue photoluminescence with a quantum yield of approximately 4.8 %. Additionally, we found that the fluorescence of the N-CQDs was intensively quenched by the addition of picric acid (PA). The decrease of the fluorescence intensity made it possible to determine PA in the linear range of 0.06–7.81 µg ml<sup>–</sup><sup>1</sup> based on the fluorescence resonance energy transfer between PA and N-CQDs. The detection limit was as low as 11.46 ng ml<sup>–</sup><sup>1</sup>. The proposed approach was further successfully applied for the determination of PA in water sample collected from Fenhe river for public safety and security, suggesting its great potential towards water routine analysis.</p
Genome-Wide Characterization of Simple Sequence Repeat (SSR) Loci in Chinese Jujube and Jujube SSR Primer Transferability
<div><p>Chinese jujube (<i>Ziziphus jujuba</i>), an economically important species in the Rhamnaceae family, is a popular fruit tree in Asia. Here, we surveyed and characterized simple sequence repeats (SSRs) in the jujube genome. A total of 436,676 SSR loci were identified, with an average distance of 0.93 Kb between the loci. A large proportion of the SSRs included mononucleotide, dinucleotide and trinucleotide repeat motifs, which accounted for 64.87%, 24.40%, and 8.74% of all repeats, respectively. Among the mononucleotide repeats, A/T was the most common, whereas AT/TA was the most common dinucleotide repeat. A total of 30,565 primer pairs were successfully designed and screened using a series of criteria. Moreover, 725 of 1,000 randomly selected primer pairs were effective among 6 cultivars, and 511 of these primer pairs were polymorphic. Sequencing the amplicons of two SSRs across three jujube cultivars revealed variations in the repeats. The transferability of jujube SSR primers proved that 35/64 SSRs could be transferred across family boundary. Using jujube SSR primers, clustering analysis results from 15 species were highly consistent with the Angiosperm Phylogeny Group (APGIII) System. The genome-wide characterization of SSRs in Chinese jujube is very valuable for whole-genome characterization and marker-assisted selection in jujube breeding. In addition, the transferability of jujube SSR primers could provide a solid foundation for their further utilization.</p></div
The primer sequences used for PCR amplification of coding sequences of 7 pulmonary arterial hypertension-associated genes.
<p>The primer sequences used for PCR amplification of coding sequences of 7 pulmonary arterial hypertension-associated genes.</p
Gene mutations in Chinese CTEPH patients and PE without PH patients.
<p>Gene mutations in Chinese CTEPH patients and PE without PH patients.</p
Large size rearrangement identified in CTEPH patients and PE without PH patients.
<p>Large size rearrangement identified in CTEPH patients and PE without PH patients.</p
categories of gene mutations in CTEPH patients and PE without PH patients.
<p>categories of gene mutations in CTEPH patients and PE without PH patients.</p
Dendrogram of 15 species based on SSR analysis.
<p>Numbers 1 to 15: Arabidopsis, eggplant, tomato, wheat, corn, Chinese cabbage, cotton, grape, apple, peach, strawberry, pear, wintersweet, jujube, and wild jujube.</p
Amplification products from 20 jujube cultivars using the JSSR97, JSSR186, and JSSR210 primers.
<p>M: 50-bp marker; numbers 1 to 20: 20 jujube cultivars listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127812#pone.0127812.t004" target="_blank">Table 4</a>.</p
Dendrogram of 20 jujube cultivars based on 16 SSRs.
<p>16 SSRs are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127812#pone.0127812.t003" target="_blank">Table 3</a>, and the 20 jujube cultivars are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127812#pone.0127812.t004" target="_blank">Table 4</a>.</p
The 20 jujube cultivars used in this experiment.
<p>The 20 jujube cultivars used in this experiment.</p
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