Additional file 1 of Population mixing mediates the intestinal flora composition and facilitates invasiveness in a globally invasive fruit fly

Additional file 1: Supplementary Information for results. Supplementary Information Fig. 1. Differences in invasiveness between the original (Fujian and Hainan) and mixed outbred populations (invasive population) of B. dorsalis. A, pupal weight; B, ovary size at 15 d after emergence; C, number of eggs laid per female per day; D, hatching rate. Supplementary Information Fig. 2. The effects of amino acid and intestinal microbe on body weight of the oriental fruit fly. A, Cephalo-pharyngeal bone length in inbred and outbred populations of B. dorsalis. B, Food intake of B. dorsalis inbred population and outbred population. C, pupal weight of inbred populations supplemented with different amino acids. D, pupa weight after feed exchange between inbred and outbred populations. Supplementary Information Fig. 3. Phenotypic differences between inbred and outbred populations of B. dorsalis. A, pupal weight; B, ovary size at 15 d after adult emergence; C, survival fraction; D, fecundity. Asterisks indicate significant differences (*,p<0.05; **, p<0.01, ***, p<0.001), and ns indicates no significant differences. Supplementary Information Fig. 4. Species compositions of the microbiomes of the intestinal flora and oviposition fluids in F populations of B. dorsalis. A, bacterial composition and relative abundance; B, fungal composition and relative abundance. The inner circle represents the oviposition fluids, and the outer circle represents the intestinal flora. Supplementary Information Fig. 5. Species compositions of the microbiomes of the intestinal flora in the inbred F and the outbred F♀×H♂ populations of B. dorsalis. A, bacterial composition and relative abundance; B, fungal composition and relative abundance. The inner circle represents the inbred F population, and the outer circle represents the outbred F♀×H♂ population. LEfSe diagram of intestinal bacteria (C) and fungi (D) between inbred F and outbred F♀×H♂ populations. Supplementary Information Fig. 6. Species compositions of the microbiomes of the intestinal flora in the inbred H and the outbred H♀×F♂ populations of B. dorsalis. A, bacterial composition and relative abundance; B, fungal composition and relative abundance. The inner circle represents the inbred H population, and the outer circle represents the outbred H♀×F♂ population. LEfSe diagram of intestinal bacteria (C) and fungi (D) between inbred H and outbred H♀×F♂ populations. Supplementary Information Table 1. The artificial diet of Bactrocera dorsalis. Supplementary Information Table 2. Life table parameters of the inbred and the outbred populations ofB. dorsalis. Supplementary Information for methods. Microbiome determination and analysis: DNA extraction and sequencing. Analysis of microbiome data. Diet exchange experiments

Prognostic value of C-reactive protein levels in patients with bone neoplasms: A meta-analysis

<div><p>Objective</p><p>The aim of this study was to conduct a meta-analysis of retrospective studies that investigated the association of preoperative C-reactive protein (CRP) levels with the overall survival (OS) of patients with bone neoplasms.</p><p>Methods</p><p>A detailed literature search was performed in the Cochrane Library, Web of Science, Embase and PubMed databases up to August 28, 2017, for related research publications written in English. We extracted the data from these studies and combined the hazard ratios (HR) and 95% confidence intervals (CIs) to assess the correlation between CRP levels and OS in patients with bone neoplasms.</p><p>Results</p><p>Five studies with a total of 816 participants from several countries were enrolled in this current meta-analysis. In a pooled analysis of all the publications, increased serum CRP levels had an adverse prognostic effect on the overall survival of patients with bone neoplasms. However, the combined data showed no significant relationship between the level of CRP and OS in Asian patients (HR = 1.73; 95% CI: 0.86–3.49; P = 0.125). Similar trends were observed in patients with bone neoplasms when stratified by ethnicity, histology, metastasis and study sample size.</p><p>Conclusions</p><p>The results of this meta-analysis suggest that increased CRP expression indicates a poorer prognosis in patients with bone neoplasms. More prospective studies are needed to confirm the prognostic significance of CRP levels in patients with bone neoplasms.</p></div

Forest plot of the association between the level of CRP and OS in patients with bone neoplasms stratified by ethnicity (A), histology (B), metastasis (C) and sample size (D).

<p>Summary of estimated hazard ratios (HRs) and 95% CI for patients stratified by (A) ethnicity, (B) histology, (C) metastasis and (D) sample size.</p

Flow chart of the study selection.

<p>Flow chart of the study selection.</p

Characteristics of all included studies.

<p>Characteristics of all included studies.</p

Begg’s funnel plot of the publication bias test for CRP level and OS in bone neoplasms.

<p>Summary of funnel plots of publication bias for the included studies. They are funnel plots of the publication bias for this meta-analysis of hazard ratios (HRs).</p

Forest plot of the association between the level of CRP and OS in patients with bone neoplasms.

<p>Summary of estimated hazard ratios (HRs) and 95% CI for patients with bone neoplasms.</p

Sensitivity analysis of the relationship between CRP level and OS in bone neoplasms.

<p>Sensitivity analyses were performed by excluding each study individually from the meta-analysis.</p

eNOS gene polymorphism analysis.

<p>A) PCR-RFLP analysis of exon 7 PCR product. B) Allele b/b of eNOS intron 4. C) Allele a/b of eNOS intron 4. D) Allele a/a of eNOS intron 4.</p