CO regulation of IAA accumulation and distribution in tomato root, leaf, and stem during lateral root development

Seedlings were grown hydroponically for 2 d after germination and then treated with the indicated concentrations of CO for 12 h. After that, the tissues were sampled and IAA was measured by HPLC. Values represent the mean of two independent experiments and vertical bars indicate the standard deviations (=90 seedlings). Asterisks indicate that the mean values are significantly different between the CO treatments and controls ( <p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

CO regulation of LR primordia initiation and LR emergence over the time

Seedlings were grown hydroponically for 2 d after germination and then treated with 0 and 10 μM CO for the indicated time. (a) Time-course of LR primordia initiation with or without CO exposure. (b) Time-course of lateral root emergence with or without CO exposure. Values represent the mean of three independent experiments and vertical bars indicate standard deviations (=45 seedlings). Asterisks indicate that the mean values are significantly different between the CO treatments and controls ( <p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

The amount of transcripts and proteins of tomato root LeHO-1 during the development of lateral roots exposed to CO

Tomato seedlings were grown hydroponically for 2 d after germination and then treated with 10 μM CO for 0, 24, 48, 72, and 96 h. (a) Analysis of transcripts by semi-quantitative RT-PCR. was used for cDNA normalization. The number below the band indicates relative abundance (RA) of with respect to the loading control actin. (b) Immunoblot analysis. Extracts from tomato roots were analysed by protein gel blotting using an antibody raised against LeHO-1 proteins. The molecular mass of the proteins is indicated on the right in kilodaltons. The results shown above were from one of the three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

Visualization of NO generation in tomato roots exposed to carbon monoxide

(a) Visualization of NO in primary roots. Seedlings were treated with CO at 0, 10, and 50 μM for 24 h. After treatments, the seedling roots were loaded with 15 μM 4,5-diaminofluorescence (DAF-2DA) for 15 min and immediately photographed (bar = 5 mm). (b) Visualization of NO in lateral roots. Seedlings were treated with 0 and 10 μM CO for 12, 24, 36, 48, and 60 h and then exposed to DAF-2DA for 15 min. After that, they were immediately photographed (bar = 10 mm).<p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

CO regulation of the lateral root development of tomato (a, b, c), Arabidopsis (ecotypes, Landsberg ) (d), and rapeseed () (e)

Tomato seedlings were grown hydroponically for 2 d after germination and then treated with the indicated concentrations of CO for 4 d. (a) Photograph of tomato lateral root formation. (b) The number of tomato lateral roots (LR) exposed to different concentrations of CO. (c) Change of elongation of tomato primary root (PR) with different levels of CO. Values represent the mean of three independent experiments and vertical bars indicate standard deviations (=45 seedlings). Asterisks indicate that the mean values are significantly different between the CO treatments and controls ( <p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

Effect of 1-naphthalene acetic acid (NAA) on the LR emergence of tomato mutant (LA2469A)

Seedlings were grown hydroponically for 2 d after germination and then treated with the indicated concentrations of NAA for 5 d. (a) The number of lateral roots (LR) exposed to the indicated concentrations of NAA. (b) Photograph of lateral root emergence. (c) Change of elongation of the primary root (PR). (d) Hypocotyl elongation. Values represent the mean of three independent experiments and vertical bars indicate standard deviations (=45 seedlings). Asterisks indicate that the mean values are significantly different between the NAA treatments and controls ( <p><b>Copyright information:</b></p><p>Taken from "Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide"</p><p></p><p>Journal of Experimental Botany 2008;59(12):3443-3452.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2529230.</p><p></p

Table_1_Predictive Role of NEK6 in Prognosis and Immune Infiltration in Head and Neck Squamous Cell Carcinoma.xlsx

Head and neck squamous cell carcinoma (HNSCC), as one of the common malignant tumors, seriously threatens human health. NEK6 (Never in Mitosis A (NIMA) related kinases 6), as a cyclin, promotes cancer cell proliferation and cancer progression. However, the prognostic value of NEK6 and its correlation with immune cell infiltration in HNSCC remain unclear. In this study, we comprehensively elucidated the prognostic role and potential function of NEK6 expression in HNSCC. The expression of NEK6 was significantly up-regulated by immunohistochemistry in HNSCC. Upregulation of NEK6 expression in gene expression studies predicts poor prognosis in HNSCC patients. The results of Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene set variation analysis indicated that NEK6 is mainly involved in extracellular matrix metabolism and EMT processes. The expression of NEK6 increased with the level of immune cell infiltration and the expression of various immune checkpoints. In conclusion, NEK6 may serve as a candidate prognostic predictor and may predict the response of HNSCC patients to immunotherapy.</p

DataSheet_2_Predictive Role of NEK6 in Prognosis and Immune Infiltration in Head and Neck Squamous Cell Carcinoma.pdf

Head and neck squamous cell carcinoma (HNSCC), as one of the common malignant tumors, seriously threatens human health. NEK6 (Never in Mitosis A (NIMA) related kinases 6), as a cyclin, promotes cancer cell proliferation and cancer progression. However, the prognostic value of NEK6 and its correlation with immune cell infiltration in HNSCC remain unclear. In this study, we comprehensively elucidated the prognostic role and potential function of NEK6 expression in HNSCC. The expression of NEK6 was significantly up-regulated by immunohistochemistry in HNSCC. Upregulation of NEK6 expression in gene expression studies predicts poor prognosis in HNSCC patients. The results of Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene set variation analysis indicated that NEK6 is mainly involved in extracellular matrix metabolism and EMT processes. The expression of NEK6 increased with the level of immune cell infiltration and the expression of various immune checkpoints. In conclusion, NEK6 may serve as a candidate prognostic predictor and may predict the response of HNSCC patients to immunotherapy.</p

DataSheet_1_Predictive Role of NEK6 in Prognosis and Immune Infiltration in Head and Neck Squamous Cell Carcinoma.csv

Head and neck squamous cell carcinoma (HNSCC), as one of the common malignant tumors, seriously threatens human health. NEK6 (Never in Mitosis A (NIMA) related kinases 6), as a cyclin, promotes cancer cell proliferation and cancer progression. However, the prognostic value of NEK6 and its correlation with immune cell infiltration in HNSCC remain unclear. In this study, we comprehensively elucidated the prognostic role and potential function of NEK6 expression in HNSCC. The expression of NEK6 was significantly up-regulated by immunohistochemistry in HNSCC. Upregulation of NEK6 expression in gene expression studies predicts poor prognosis in HNSCC patients. The results of Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene set variation analysis indicated that NEK6 is mainly involved in extracellular matrix metabolism and EMT processes. The expression of NEK6 increased with the level of immune cell infiltration and the expression of various immune checkpoints. In conclusion, NEK6 may serve as a candidate prognostic predictor and may predict the response of HNSCC patients to immunotherapy.</p