8 research outputs found
The localities of the natural <i>D</i>. <i>kaki var</i>. <i>silvestris</i> Mak samples.
<p>The localities of the natural <i>D</i>. <i>kaki var</i>. <i>silvestris</i> Mak samples.</p
Investigation and Analysis of Genetic Diversity of Diospyros Germplasms Using SCoT Molecular Markers in Guangxi
<div><p>Background</p><p>Knowledge about genetic diversity and relationships among germplasms could be an invaluable aid in diospyros improvement strategies.</p><p>Methods</p><p>This study was designed to analyze the genetic diversity and relationship of local and natural varieties in Guangxi Zhuang Autonomous Region of China using start codon targeted polymorphism (SCoT) markers. The accessions of 95 diospyros germplasms belonging to four species <i>Diospyros kaki</i> Thunb, <i>D</i>. <i>oleifera</i> Cheng, <i>D</i>. <i>kaki</i> var. <i>silverstris</i> Mak, and <i>D</i>. <i>lotus</i> Linn were collected from different eco-climatic zones in Guangxi and were analyzed using SCoT markers.</p><p>Results</p><p>Results indicated that the accessions of 95 diospyros germplasms could be distinguished using SCoT markers, and were divided into three groups at similarity coefficient of 0.608; these germplasms that belong to the same species were clustered together; of these, the degree of genetic diversity of the natural <i>D</i>. <i>kaki</i> var. <i>silverstris</i> Mak population was richest among the four species; the geographical distance showed that the 12 natural populations of <i>D</i>. <i>kaki</i> var. <i>silverstris</i> Mak were divided into two groups at similarity coefficient of 0.19. Meanwhile, in order to further verify the stable and useful of SCoT markers in diospyros germplasms, SSR markers were also used in current research to analyze the genetic diversity and relationship in the same diospyros germplasms. Once again, majority of germplasms that belong to the same species were clustered together. Thus SCoT markers were stable and especially useful for analysis of the genetic diversity and relationship in diospyros germplasms.</p><p>Discussion</p><p>The molecular characterization and diversity assessment of diospyros were very important for conservation of diospyros germplasm resources, meanwhile for diospyros improvement.</p></div
UPGMA dendrogram of the accessions of 95 diospyros germplasms based on SSR molecular markers.
<p>UPGMA dendrogram of the accessions of 95 diospyros germplasms based on SSR molecular markers.</p
Analysis of molecular variance (AMOVA) within and among natural <i>D</i>. <i>kaki</i> var. <i>silvestris</i> Mak. populations.
<p>Note: MSD,expected mean squares</p><p>*Number of permutation = 1,000</p><p>Analysis of molecular variance (AMOVA) within and among natural <i>D</i>. <i>kaki</i> var. <i>silvestris</i> Mak. populations.</p
Results of analysis of the genetic diversity of the 12 natural <i>D</i>. <i>kaki</i> var. <i>silverstris</i> Mak. populations.
<p>Results of analysis of the genetic diversity of the 12 natural <i>D</i>. <i>kaki</i> var. <i>silverstris</i> Mak. populations.</p
UPGMA dendrogram of the natural <i>D</i>. <i>kaki var</i>. <i>silvestris</i> Mak populations based on Nei’s genetic distance.
<p>UPGMA dendrogram of the natural <i>D</i>. <i>kaki var</i>. <i>silvestris</i> Mak populations based on Nei’s genetic distance.</p
Diospyros germplasm resources and their respective localities.
<p>Note: The germplasms that have not been previously reported, or whose names are not sure were temporarily named after the first letter of their localities and numbers.</p><p>Diospyros germplasm resources and their respective localities.</p
UPGMA dendrogram of the accessions of 95 diospyros germplasms based on SCoT molecular markers.
<p>UPGMA dendrogram of the accessions of 95 diospyros germplasms based on SCoT molecular markers.</p