Activation of Caspase-3 induced by T-2 toxin.

<p><b>A</b>. Detection of Caspase-3 hydrolase activity under T-2 toxin stress. <b>B</b>. Western blot result of Caspase-3 activated fragments in Hela cells when treated with T-2 at the concentration of LC50 for 8, 16, and 24 h respectively. <b>C</b>. Western blot result of Caspase-3 activated fragments in Changliver cells. <b>D</b>. Caspase-3 activity in Bel -7402 cell. Data was presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001.</p

The effect of up-regulated JunD on cells.

<p><b>A</b>. The expression levels of JunD induced by T-2 toxin. <b>B</b>. The expression of JunD after transfection of JunD over-expression vector in three cell strains. wt: wild type. +: cell strains transfected with over-expression vector. <b>C</b>. The effect of over-expressed JunD on cell survival rate. T-2: wild-type cells under T-2 toxin stress. JunD (+/+): JunD over-expressied cell lines under T-2 toxin stress. Data was presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001.</p

Intracellular redox level under T-2 toxin.

<p>A. Level of endogenous GSH under T-2 toxin stress. B. Levels of endogenous MDA under T-2 toxin stress. Data was presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001.</p

The inhibition effect of T-2 toxin on three cell strains.

<p><b>A</b>. Dose-dependent inhibition ration of T-2 toxin at 24 h. <b>B</b>. Time-dependent effect of T-2 toxin to cells. Data was presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001.</p

Antioxidation of NAC in cell death induced by T-2 toxin.

<p><b>A</b>. Antioxidation of NAC in the cell death process of Hela cells. <b>B</b>. Endogenous GSH level of Hela under protection of NAC. Data was presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001.</p

Western-blot analysis on activated Caspase-8 and Caspase-9 level.

<p><b>A</b>. Activated Caspase-8 and Caspase-9 level in Hela cells when treated with T-2 toxin at the concentration of LC50 for 8, 16, and 24 h respectively. <b>B</b>. Activated Caspase-8 and Caspase-9 level in Changliver cells were treated with T-2 toxin. <b>C</b>. Bel -7402 cells were treated with T-2 toxin.</p