«Conselho de amigo, aviso do céu»: contributos para a análise semântico-pragmática dos atos ilocutórios de conselho e de aviso em confronto com o de ameaça

Com base em exemplos de um corpus oral de Português Europeu, o presente texto discute a organização e funcionamento do ato ilocutório de conselho e de aviso, destacando as semelhanças e diferenças entre eles e os atos de discursos como ameaças e promessas, avisos hipotéticos e ameaças condicionais. Depois da análise das noções do “querer dizer NN” de Grice e de “uptake” de Austin, destacamos a importância da relação entre intenção e convenção na análise dos atos ilocutórios de Searle com o levantamento das regras que determinam os indicadores de força ilocutória. A interpretação dos valores ilocutórios na sequencialidade das intervenções destaca a relação entre práticas discursivas e figuração.info:eu-repo/semantics/publishedVersio

Comparisons of the cDNA sequence of the <i>PsMLO1</i> gene from the wild-type pea cultivar Sprinter (Wild_type_PsMLO1) and Bawan 6 (BW6_PsMLO1_cDNA), which are susceptible to <i>E</i>. <i>pisi</i>, and the 16 resistant pea landraces.

<p>Mutated sites in the <i>PsMLO1</i> cDNA sequence are boxed in red.</p

Genetic linkage map of the F₂ population derived from G0001778 × Bawan 6 with the linked markers AD60 and c5DNAmet, showing the position of the resistance gene for powdery mildew.

<p>Map distances and loci order were determined using MAPMAKER 3.0 (Lander et al. 1993). Estimated genetic distances between loci are shown on the left in centiMorgans (cM).</p

Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene <i>Rps10</i> in a Chinese Soybean Cultivar

<div><p>Resistance to <i>Phytophthora sojae</i> isolate PsMC1 was evaluated in 102 F_2∶3 families derived from a cross between the resistant soybean cultivar Wandou 15 and the susceptible cultivar Williams and genotyped using simple sequence repeat (SSR) markers. The segregation ratio of resistant, segregating, and susceptible phenotypes in the population suggested that the resistance in Wandou 15 was dominant and monogenic. Twenty-six polymorphic SSR markers were identified on soybean chromosome 17 (Molecular linkage group D2; MLG D2), which were linked to the resistance gene based on bulked segregation analysis (BSA). Markers Sattwd15-24/25 and Sattwd15-47 flanked the resistance gene at a distance of 0.5 cM and 0.8 cM, respectively. Two cosegregating markers, Sattwd15-28 and Sattwd15-32, were also screened in this region. This is the first <i>Rps</i> resistance gene mapped on chromosome 17, which is designated as <i>Rps10</i>. Eight putative genes were found in the mapped region between markers Sattwd15-24/25 and Sattwd15-47. Among them, two candidate genes encoding serine/threonine (Ser/Thr) protein kinases in Wandou 15 and Williams were identified and sequenced. And the differences in genomic sequence and the putative amino acid sequence, respectively, were identified within each candidate gene between Wandou 15 and Williams. This novel gene <i>Rps10</i> and the linked markers should be useful in developing soybean cultivars with durable resistance to <i>P</i>. <i>sojae</i>.</p></div

Photomicrographs of rabbit spermatozoa.

<p>(A-C) Images obtained using phase contrast microscope; (D-F) Images, which were the same images as (A-C) respectively, obtained by staining with the probe BODIPY 581/591C11. Three kinds of staining could be observed under a fluorescence microscope: (D) indicates that sperm was seriously oxidized; (E) sperm was partly oxidized; (F) sperm was not oxidized. Bars = 15μm.</p

Structures of the gene models Glyma17g28950.1 and Glyma17g28970.1, the candidate genes <i>Rps10-1</i> and <i>Rps10-2</i>, and the putative proteins Rps10-1 and Rps10-2.

<p>(A) The structures of the gene models Glyma17g28950.1, Glyma17g28970.1 from 309×10⁵ to 310×10⁵ bp on chromosome 17 of Williams 82. (B) Predicated structure of the candidate gene <i>Rps10-1</i>. (C) Predicted structure of the putative protein Rps10-1. (D) Predicated structure of the candidate gene <i>Rps10-2</i>. (E) Predicted structure of the putative protein Rps10-2. Filled rectangular arrows with orientations (from 5′ to 3′) indicate the predicated genes. Filled rectangles indicate the exons. Open rectangular arrows with orientations (N-C) indicate the predicated proteins. The filled rectangle indicates the Ser/Thr protein kinase domain.</p

Detection of addition of vitamin E analogue to freezing extender on lipid peroxidation in the frozen-thawed rabbit spermatozoa.

<p>Sperm lipid peroxidation was analyzed by staining with BODIPY 581/591C₁₁. Histogram shows spermatozoa with lipid peroxidation (marker M1) and without lipid peroxidation (marker M2). A: 0 μM; B: 100 μM; C: 200 μM; D: 250 μM.</p

High-resolution melting (HRM) curve profiles of amplicons produced by the SNP marker SNP1121.

<p>A. HRM showing three distinct curves in F₂ individuals derived from G0001778 (C) × Bawan 6 (T), corresponding resistant homozygotes, susceptible homozygotes and heterozygotes, respectively. B. HRM showing two different curve profiles in resistant pea landraces carrying <i>er1</i>-6 alleles (C), and both susceptible and resistant pea germplasms respectively carrying <i>Er1</i> (T) and other <i>er1</i> alleles (T).</p

Phytophthora resistance phenotype of the soybean cultivars Wandou 15 and Williams were tested with <i>Phytophthora sojae</i> isolate PsMC1 using the hypocotyl-inoculation technique.

<p>Phytophthora resistance phenotype of the soybean cultivars Wandou 15 and Williams were tested with <i>Phytophthora sojae</i> isolate PsMC1 using the hypocotyl-inoculation technique.</p

Effect of supplementation TCG extender with Trolox on mitochondrial membrane potential of the frozen-thawed rabbit spermatozoa.

<p>Mitochondrial membrane potential was evaluated using JC-1 (lipophilic cation 5,5’, 6,6’- tetrachloro-1,1’,3,3’ -tetraethylbenzimidazolcarbocyanine iodide) Mitochondrial Membrane Potential Detection Kit. Histogram shows spermatozoa with high (marker M2) and low (marker M1) mitochondrial membrane potential. (A: 0 μM; B: 100 μM; C: 200 μM; D: 250 μM).</p