Additional file 1 of GSH-responsive degradable nanodrug for glucose metabolism intervention and induction of ferroptosis to enhance magnetothermal anti-tumor therapy

Additional file 1: Figure S1. Representative TEM images of LFMP. Figure S2. T2-weighted MR imaging (A) and transverse relaxivity (r2) of LFMP was examined by a clinical 3.0 T MR imaging device with a T2 mapping sequence (B). The quantitative assay was performed by measuring the intensity of MR images using ImageJ software (C). Data shown as mean ± SD, n = 5, **p < 0.01, ***p < 0.001. Figure S3. Biocompatibility of different concentrations of LFMP in blood for 4 h. H2O as positive control, 0.9% NaCl as negative control. Figure S4. Representative TEM images of biodegradation behavior of LFMP in PBS solution ([GSH] = 10 mM) for 10 days (scale bar = 50 μm). Figure S5. Half-maximal inhibitory concentration (IC50) of LND (A), FMP (B), FMP + AMF (B), LFMP (C), and LFMP + AMF(C) in EMT-6 cells. The data are presented as the mean ± SD, n = 6. Figure S6. Combination index plot for drug combination of LND and FMP. Figure S7. TUNEL staining and the corresponding proportion of TUNEL positive cells of tumor sections after the survival experiment (Scale bar = 100 μm). The data was shown as mean ± SD, n = 6 per group, ***p < 0.001. Figure S8. Blood biochemical indexes and hematology parameters of the mice with different treatments. The data are presented as the mean ± SD, n = 6. Table S1. Physicochemical properties of FM and LFMP. Table S2. Hydrodynamic diameter distributions and Zeta potential of LFMP in different solutions. Data are presented as mean ± SD, n = 6

Media 1.mp4

The instantaneous magnetic field distribution movie at 1.86GHz

Global characterization of the Dicer-like protein DrnB roles in miRNA biogenesis in the social amoeba <i>Dictyostelium discoideum</i>

Micro (mi)RNAs regulate gene expression in many eukaryotic organisms where they control diverse biological processes. Their biogenesis, from primary transcripts to mature miRNAs, have been extensively characterized in animals and plants, showing distinct differences between these phylogenetically distant groups of organisms. However, comparably little is known about miRNA biogenesis in organisms whose evolutionary position is placed in between plants and animals and/or in unicellular organisms. Here, we investigate miRNA maturation in the unicellular amoeba Dictyostelium discoideum, belonging to Amoebozoa, which branched out after plants but before animals. High-throughput sequencing of small RNAs and poly(A)-selected RNAs demonstrated that the Dicer-like protein DrnB is required, and essentially specific, for global miRNA maturation in D. discoideum. Our RNA-seq data also showed that longer miRNA transcripts, generally preceded by a T-rich putative promoter motif, accumulate in a drnB knock-out strain. For two model miRNAs we defined the transcriptional start sites (TSSs) of primary (pri)-miRNAs and showed that they carry the RNA polymerase II specific m7G-cap. The generation of the 3ʹ-ends of these pri-miRNAs differs, with pri-mir-1177 reading into the downstream gene, and pri-mir-1176 displaying a distinct end. This 3´-end is processed to shorter intermediates, stabilized in DrnB-depleted cells, of which some carry a short oligo(A)-tail. Furthermore, we identified 10 new miRNAs, all DrnB dependent and developmentally regulated. Thus, the miRNA machinery in D. discoideum shares features with both plants and animals, which is in agreement with its evolutionary position and perhaps also an adaptation to its complex lifestyle: unicellular growth and multicellular development.</p

Presentation1_Underestimated increase and intensification of humid-heat extremes across southeast China due to humidity data inhomogeneity.zip

When co-occurring with elevated levels of ambient relative humidity (RH), hot extremes are more perceivable and consequently more health-damaging. Quantifying changes in humid-heat extremes has therefore gained considerable scientific and societal attention, but a fundamental yet critical aspect to the estimation—data reliability—has been largely downplayed in previous analysis. By comparing ∼10 observational and reanalysis datasets to fully-homogenized observations across China, we report ubiquitous inhomogeneity in RH series in these popularly-used datasets [including HadISD(H) and ERA5], which accordingly produce unrealistically strong drying trends 2–3 times the homogenized dataset-based estimate during 1979–2013 in warm-moist southeast China. Locally, an inhomogeneity-caused exaggeration of drying by a magnitude of 1% decade−1 translates into a significant underestimation of increasing rates for frequency and intensity of humid-heat extremes by more than 1.2 days decade−1 and .07% decade−1 respectively. From a regional perspective, these inhomogeneous records have underestimated the frequency increase of extremes by up to 2 days decade−1 and their intensification by up to .4°C decade−1 in southeast China. Extremes identified via homogenized and non-homogenized datasets also differ in the bivariate joint distribution structure, with former cases featuring similarly hot temperatures yet discernably lower humidity.</p

Community composition of soil bacteria at the phylum level.

Community composition of soil bacteria at the phylum level.</p

Community composition of soil bacteria at the genus level.

Community composition of soil bacteria at the genus level.</p

Soil basic properties under different treatments.

Soil basic properties under different treatments.</p

PCA analysis of soil bacterial communities under different fertilization treatments.

PCA analysis of soil bacterial communities under different fertilization treatments.</p

RDA analysis of the soil bacterial community and soil properties.

RDA analysis of the soil bacterial community and soil properties.</p

Effect of different treatments on pakchoi nutrients.

Effect of different treatments on pakchoi nutrients.</p