Pseudo Mask Augmented Object Detection

In this work, we present a novel and effective framework to facilitate object detection with the instance-level segmentation information that is only supervised by bounding box annotation. Starting from the joint object detection and instance segmentation network, we propose to recursively estimate the pseudo ground-truth object masks from the instance-level object segmentation network training, and then enhance the detection network with top-down segmentation feedbacks. The pseudo ground truth mask and network parameters are optimized alternatively to mutually benefit each other. To obtain the promising pseudo masks in each iteration, we embed a graphical inference that incorporates the low-level image appearance consistency and the bounding box annotations to refine the segmentation masks predicted by the segmentation network. Our approach progressively improves the object detection performance by incorporating the detailed pixel-wise information learned from the weakly-supervised segmentation network. Extensive evaluation on the detection task in PASCAL VOC 2007 and 2012 [12] verifies that the proposed approach is effective

PGE2 Elevates IL-23 Production in Human Dendritic Cells via a cAMP Dependent Pathway

PGE2 elevates IL-23 production in mouse dendritic cells while inhibits IL-23 production in isolated human monocytes. Whether this differential effect of PGE2 on IL-23 production is cell-type- or species-specific has not been investigated in detail. The present study was designed to investigate the effect of PGE2 on IL-23 production in human DCs and the possible underlying mechanisms. Human monocytes derived dendritic cells (Mo-DCs) were pretreated with or without PGE2. Then the cells were incubated with zymosan. Our results demonstrated that PGE2 promoted zymosan-induced IL-23 production in a concentration dependent manner. In addition, it was found that PGE2 is also able to elevate MyD88-mediated IL-23 p19 promoter activity. More importantly, ELISA data demonstrated that db-cAMP, a cAMP analog, and forskolin, an adenylate cyclase activator, can mimic the effect of PGE2 on zymosan-induced IL-23 production, and rp-cAMP, a protein kinase A (PKA) inhibitor, can block the effect of PGE2. Moreover, PGE2 can increase zymosan-induced expression of the mRNA levels of both p19 and p40 subunits, which was mimicked by db-cAMP and forskolin. Our data suggest that PGE2 elevates the production of IL-23 in human Mo-DCs via a cAMP dependent pathway.</jats:p