Assembly of Peptide with Dye Molecules for the Fabrication of Colorimetric Biosensor with Application To Diagnose HER2-Positive Breast Cancer

Peptide has been widely used for sensor design, but signal output and amplification have been challenges for such types of biosensors. In this work, a coassembly strategy has been adopted to fabricate a colorimetric biosensor with simple signal convertor and amplification. Meanwhile, by regulating the coassembly peptosome with optimal size, a sensitive and specific colorimetric biosensor can be fabricated. As a proof-of-concept, human epidermal growth factor receptor 2 (HER2) protein as the target can be specifically bound by the peptosome, and the molecular recognition events can subsequently be turned into visual signal output; thus, HER2 protein can be quantified with a limit of detection (LOD) of 3 ng mL–1. Moreover, experimental results can validate the capability of the biosensor in identifying HER2-positive and HER2-negative breast cancer patients. Therefore, this biosensor may have great potential in the field of biosensor-based in vitro diagnosis

DataSheet_1_A global gridded ocean salinity dataset with 0.5° horizontal resolution since 1960 for the upper 2000 m.docx

A gridded salinity dataset with high resolution is essential for investigating global ocean salinity variability and understanding its role in climate and the ocean ecosystem. In this study, a new version of the Institute of Atmospheric Physics gridded salinity dataset with a higher resolution (0.5° by 0.5°) is provided by using a revised ensemble optimal interpolation scheme with a dynamic ensemble. The performance of this dataset is evaluated using “subsample test” and the high-resolution satellite-based data. Compared with the previous 1° by 1° resolution IAP product, the new dataset is more capable of representing regional salinity changes with the meso-scale and small-scale signals (i.e., in the coastal and boundary currents regions), meanwhile, maintains the large-scale structure and variability. Therefore, the new dataset complements the previous data product. Besides, the new dataset is compared with in situ observations and several international salinity products for the salinity multiscale variabilities and patterns. The comparison shows the smaller magnitude of mean difference and Root-mean-square deviation (RMSD) in basin scale for the new dataset, some differences in strength and fine structure of the “fresh gets fresher, salty gets saltier” surface and subsurface salinity pattern amplification trends from 1980 to 2017, a broad similarity for the salinity changes associated with El Niño-Southern Oscillation (ENSO) and a consistent salinity dipole mode in the tropical Indian Ocean (S-IOD). These results support the future use of gridded salinity data.</p