3 research outputs found
Glyco-Nanovesicles with Activatable Near-Infrared Probes for Real-Time Monitoring of Drug Release and Targeted Delivery
A glyco-nanovesicle
(Lac-SS-DCM) is self-assembled by a rationally
designed amphiphilic lactose derivative (<b>1</b>), which features
a surface lactose corona, a disulfide linkage, and an activatable
DCM near-infrared (NIR) probe moiety. Taking advantage of the disulfide
linkage, Lac-SS-DCM can be triggered to disassemble by glutathione
(GSH) and simultaneously activate the dormant NIR, which allows for
a drug-loaded vesicle capable of both therapies in cancer cells where
a higher GSH concentration exists and real-time monitoring of drug
release. Furthermore, Lac-SS-DCM demonstrates excellent HepG2 target
ability as well as higher anticancer efficacy and reduced side effects
compared to those of free DOX through lactose-mediated endocytosis
resulting from the surface lactose corona, which acts as a multivalent
galectin-targeting ligand. As a multifunctional drug delivery compound
with perfect synchronization of targeting, imaging, monitoring, and
controllable drug release, we believe this activatable glyco-nanovesicle,
readily modulated for imaging of different tumors by incorporation
of unique targeting entities on the vesicle surface, would be of broad
interest for cancer diagnosis and therapy
One-Step Synthesis of Dual Clickable Nanospheres via Ultrasonic-Assisted Click Polymerization for Biological Applications
Dual clickable nanospheres (DCNSs)
were synthesized in one step using an efficient approach of ultrasonic-assisted
azide–alkyne click polymerization, avoiding the need of surfactants.
This novel approach presents a direct clickable monomer-to-nanosphere
synthesis. Field emission scanning electron microscopy (FESEM), Fourier
transform infrared spectroscopy (FTIR), and dynamic laser scattering
(DLS) were used to characterize the synthesized DCNSs. Numerous terminal
alkynyl and azide groups on the surface of DCNSs facilitate effective
conjugation of multiple molecules or ligands onto a single nanocarrier
platform under mild conditions. To exemplify the potential of DCNSs
in biological applications, (1) multivalent glyconanoparticles (GNPs)
were prepared by clicking DCNSs with azide-functionalized and alkyne-functionalized
lactose sequentially for the determination of carbohydrate-galectin
interactions with quartz crystal microbalance (QCM) biosensor. Using
protein chip (purified galectin-3 coated on chip) and cell chip (Jurkat
cells immobilized on chip), the QCM sensorgrams showed excellent binding
activity of GNPs for galectins; (2) fluorescent GNPs were prepared
by clicking DCNSs with azide-functionalized Rhodamine B and alkyne-functionalized
lactose sequentially in order to target galectin, which is overexpressed
on the surface of Jurkat cells. The fluorescent images obtained clearly
showed the cellular internalization of fluorescent GNPs. This fluorescent
probe could be easily adapted to drugs to construct lectin-targeted
drug delivery systems. Thus, DCNSs prepared with our method may provide
a wide range of potential applications in glycobiology and biomedicine
Supramolecular Vesicles Based on Complex of Trp-Modified Pillar[5]arene and Galactose Derivative for Synergistic and Targeted Drug Delivery
Supramolecular Vesicles Based on Complex of Trp-Modified
Pillar[5]arene and Galactose Derivative for Synergistic and Targeted
Drug Deliver