26 research outputs found

    Additional file 1: of Transcriptomic and metabolomic analyses reveal that bacteria promote plant defense during infection of soybean cyst nematode in soybean

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    Table S1. Comparison of expression profiles of random selected 21 genes by RNA-seq and qRT-PCR data. Twenty one genes were randomly selected from the genes differentially regulated, and examined for their expression at 5 days post-inoculation using. The results were the average from three biological replicates. (XLSX 12 kb

    Additional file 7: of Transcriptomic and metabolomic analyses reveal that bacteria promote plant defense during infection of soybean cyst nematode in soybean

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    Table S6. Comprehensive networks of metabolites and transcripts, changed in common KEGG pathways and differentially expressed genes among these pathways. (XLSX 13 kb

    Additional file 3: of Transcriptomic and metabolomic analyses reveal that bacteria promote plant defense during infection of soybean cyst nematode in soybean

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    Table S3.Statistics of reads mapped back to the reference transcriptome. Total reads: the sequencing sequence has been filtered by the sequencing data (Clean data). Total mapped: the number of sequences that can be located on the genome; n general, if there is no pollution and the reference genome is appropriate, the percentage of this data is greater than 70%. (XLSX 9 kb

    Identification of Soybean MicroRNAs Involved in Soybean Cyst Nematode Infection by Deep Sequencing

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    <div><p>Soybean cyst nematode (SCN), <em>Heterodera glycines</em>, is the most devastating pathogen of soybean worldwide. MicroRNAs (miRNAs) are a class of small, non-coding RNAs that are known to play important role in plant stress response. However, there are few reports profiling the miRNA expression patterns during pathogen stress. We sequenced four small RNA libraries from two soybean cultivar (Hairbin xiaoheidou, SCN race 3 resistant, Liaodou 10, SCN race 3 susceptible) that grown under un-inoculated and SCN-inoculated soil. Small RNAs were mapped to soybean genome sequence, 364 known soybean miRNA genes were identified in total. In addition, 21 potential miRNA candidates were identified. Comparative analysis of miRNA profiling indicated 101 miRNAs belong to 40 families were SCN-responsive. We also found 20 miRNAs with different express pattern even between two cultivars of the same species. These findings suggest that miRNA paly important role in soybean response to SCN and have important implications for further identification of miRNAs under pathogen stress.</p> </div

    Novel miRNA candidates predicted from miRNA precursor.

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    <p>Novel miRNA candidates predicted from miRNA precursor.</p

    The S<sub>50</sub> values of <i>M</i>. <i>hapla</i> J2 subjected to various regimes.

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    <p>FR = freezing regime; CD-6 h = Cryoprotective dehydration regime that held for 6 h at -1┬░C before cooled to T<sub>min</sub>, CS = cold shock at -1┬░C for 1 h and then kept at room temperature for 1 h before cooled to T<sub>min</sub>, A4 = acclimated at 4┬░C for 12 h before cooled to T<sub>min</sub>. The bars are the estimations in the 95% confidence limits. The different lowercase letters on the bars represent significantly different among various regimes, according to RMP estimates. N = 10.</p
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