Overexpression of BRD7 inhibited lung adenocarcinoma cell proliferation and migration.

<p>(A) The protein expression level of BRD7 was measured by using Western blot. (B) The mRNA expression level of BRD7 was measured by using qRT-PCR. (C) Overexpression of BRD7 inhibited lung adenocarcinoma cell line A549 proliferation. (D) Overexpression of BRD7 inhibited lung adenocarcinoma cell line A549 migration. (E) Relative ratio of wound closure per field is shown. *p<0.05 and ***p<0.001.</p

The protein expression level of BRD7 was downregulated in lung adenocarcinoma tissues.

<p>(A) Lower of BRD7 levels in patients with lung adenocarcinoma was associated with shortened disease-free survival (hazards ratio = 0.36). (B) The protein expression level of BRD7 was measured in lung adenocarcinoma tissues and their corresponding adjacent normal tissues using Western blot.</p

Inhibition of BRD7 promoted ERK phosphorylation in lung adenocarcinoma.

<p>(A) The protein expression level of BRD7 was measured by using Western blot. (B) The mRNA expression level of BRD7 was measured by using qRT-PCR. (C) Inhibition of BRD7 promoted the ERK phosphorylation in the A549 cells. (D) CCK8 analysis was used to measure the A549 cell proliferation. (E) Overexpression of BRD7 suppressed the ERK phosphorylation in the A549 cells. (F) Wound-healing assay was performed to measure the A549 cell migration. (G) Relative ratio of wound closure per field is shown. *p<0.05 and ***p<0.001.</p

The expression level of BRD7 was downregulated in lung adenocarcinoma cell lines.

<p>(A) The protein expression level of BRD7 in four lung adenocarcinoma cell lines (H1299, H23, SPC-A1 and A549) and one lung adenocarcinoma tissues and adjacent no-tumor tissue was measured by Western blot. (B) The signal in each lane was quantified using ImageJ software and the ratio of BRD7 to GAPDH was determined. (C)The mRNA expression level of BRD7 in four lung adenocarcinoma cell lines (H1299, H23, SPC-A1 and A549) and one lung adenocarcinoma tissues and adjacent no-tumor tissue was measured by qRT-PCR.</p