1 research outputs found
Cell Membrane and V<sub>2</sub>C MXene-Based Electrochemical Immunosensor with Enhanced Antifouling Capability for Detection of CD44
The inactive adsorption and interference of biomolecules
in electrochemical
biosensors is a topic of intense interest. Directly utilizing native
cell membranes to endow electrochemical surfaces with antifouling
and biocompatible features is a promising strategy, rather than attempting
to synthetically replicate complex biological interface properties.
In this study, we present a facial and sensitive sandwich-type antifouling
immunoassay through platelet membrane/Au nanoparticle/delaminated
V2C nanosheet (PM/AuNPs/d-V2C)-modified electrode
as the substrate of sensing interface and methylene blue/aminated
metal organic framework (MB@NH2-Fe-MOF-Zn) as an electrochemical
signal probe. The biosensor perfectly integrates the high conductivity
of AuNPs-loaded V2C MXene with the excellent loading property
of NH2-Fe-MOF-Zn to improve the electrochemical sensing
performance. In addition, the excellent antifouling properties of
the homogeneous cell membrane can effectively prevent the non-specific
adsorption of model proteins. The obtained antifouling biosensor possesses
the capability of ultrasensitive detection of CD44 and CD44-positive
cancer cell in complex liquids and exhibits good analytical performance
for the analysis of CD44 with a linear range from 0.5 ng/mL to 500
ng/mL. This strategy of developing cell membrane-based biosensing
systems with enhanced antifouling capability can be easily expanded
to the construction of other complex biosensors, and the advanced
biological probes and analytical methods provide a favorable means
to accurately quantify biomarkers associated with tumor progression