SESNtemple: First Public Release

<p>First public release of average spectra and standard deviation spectra of all SN IIb, Ib, Ic, Ic-bl (including SN-GRBs) from the literature, at different phases, and their corresponding SuperNova IDentification templates.</p

Classification of coupling coordination degree.

In 2014, the Chinese government unveiled the New Urbanization Plan and Document No. 46, which profoundly influenced the development trajectory of the regional economy and sports industry. Using the coupling coordination model, this study aimed to assess the development progress of the sports industry and urban clusters economy. This study sampled Greater Bay Area urban clusters (GBAUC) and Yangtze River Delta urban clusters (YRDUC). The statistics covered one year after the release of the policies to date. We developed a total of 15 macro indicators to evaluate the sports industry and urban cluster economy as two distinct, yet interdependent, economic systems. Using the entropy weight method, we determined the standardized values and weights for the two systems before calculating the coupling coordination degree (D). Between 2015 and 2021, the sampled sports industry and urban clusters economy exhibited coordinated high growth across all economic metrics, with multiple sports industry metrics exhibiting double-digit growth. In 2015, both showed extreme imbalance: D of GBAUC = 0.092, D of YRDUC = 0.091. In 2017, both improved to bare coordination: D of GBAUC = 0.600, D of YRDUC = 0.566. In 2019, both reached well coordination: D of GBAUC = 0.851, D of YRDUC = 0.814. By 2021, both achieved quality coordination: D of GBAUC = 0.990, D of YRDUC = 1. This study provides the first evidence from the sports industry that China’s new urbanization model and Document No. 46 are highly effective for synergistic regional economic growth.</div

The coupling coordination degree of the sports industry and urban clusters economy from 2015 to 2021: (A) Greater Bay Area urban clusters; (B) Yangtze River Delta urban clusters.

The grey color represents a lack of data in the region.</p

Strand Displacement Amplification Reaction on Quantum Dot-Encoded Silica Bead for Visual Detection of Multiplex MicroRNAs

The combination of microbead array, isothermal amplification, and molecular signaling enables the continuous development of next-generation molecular diagnostic techniques. Herein we reported the implementation of nicking endonuclease-assisted strand displacement amplification reaction on quantum dots-encoded microbead (Qbead), and demonstrated its feasibility for multiplexed miRNA assay in real sample. The Qbead featured with well-defined core–shell superstructure with dual-colored quantum dots loaded in silica core and shell, respectively, exhibiting remarkably high optical encoding stability. Specially designed stem-loop-structured probes were immobilized onto the Qbead for specific target recognition and amplification. In the presence of low abundance of miRNA target, the target triggered exponential amplification, producing a large quantity of stem-G-quadruplexes, which could be selectively signaled by a fluorescent G-quadruplex intercalator. In one-step operation, the Qbead-based isothermal amplification and signaling generated emissive “core-shell-satellite” superstructure, changing the Qbead emission-color. The target abundance-dependent emission-color changes of the Qbead allowed direct, visual detection of specific miRNA target. This visualization method achieved limit of detection at the subfemtomolar level with a linear dynamic range of 4.5 logs, and point-mutation discrimination capability for precise miRNA analyses. The array of three encoded Qbeads could simultaneously quantify three miRNA biomarkers in ∼500 human hepatoma carcinoma cells. With the advancements in ease of operation, multiplexing, and visualization capabilities, the isothermal amplification-on-Qbead assay could potentially enable the development of point-of-care diagnostics

Overview of the evaluation index system.

In 2014, the Chinese government unveiled the New Urbanization Plan and Document No. 46, which profoundly influenced the development trajectory of the regional economy and sports industry. Using the coupling coordination model, this study aimed to assess the development progress of the sports industry and urban clusters economy. This study sampled Greater Bay Area urban clusters (GBAUC) and Yangtze River Delta urban clusters (YRDUC). The statistics covered one year after the release of the policies to date. We developed a total of 15 macro indicators to evaluate the sports industry and urban cluster economy as two distinct, yet interdependent, economic systems. Using the entropy weight method, we determined the standardized values and weights for the two systems before calculating the coupling coordination degree (D). Between 2015 and 2021, the sampled sports industry and urban clusters economy exhibited coordinated high growth across all economic metrics, with multiple sports industry metrics exhibiting double-digit growth. In 2015, both showed extreme imbalance: D of GBAUC = 0.092, D of YRDUC = 0.091. In 2017, both improved to bare coordination: D of GBAUC = 0.600, D of YRDUC = 0.566. In 2019, both reached well coordination: D of GBAUC = 0.851, D of YRDUC = 0.814. By 2021, both achieved quality coordination: D of GBAUC = 0.990, D of YRDUC = 1. This study provides the first evidence from the sports industry that China’s new urbanization model and Document No. 46 are highly effective for synergistic regional economic growth.</div

Evaluation index system.

In 2014, the Chinese government unveiled the New Urbanization Plan and Document No. 46, which profoundly influenced the development trajectory of the regional economy and sports industry. Using the coupling coordination model, this study aimed to assess the development progress of the sports industry and urban clusters economy. This study sampled Greater Bay Area urban clusters (GBAUC) and Yangtze River Delta urban clusters (YRDUC). The statistics covered one year after the release of the policies to date. We developed a total of 15 macro indicators to evaluate the sports industry and urban cluster economy as two distinct, yet interdependent, economic systems. Using the entropy weight method, we determined the standardized values and weights for the two systems before calculating the coupling coordination degree (D). Between 2015 and 2021, the sampled sports industry and urban clusters economy exhibited coordinated high growth across all economic metrics, with multiple sports industry metrics exhibiting double-digit growth. In 2015, both showed extreme imbalance: D of GBAUC = 0.092, D of YRDUC = 0.091. In 2017, both improved to bare coordination: D of GBAUC = 0.600, D of YRDUC = 0.566. In 2019, both reached well coordination: D of GBAUC = 0.851, D of YRDUC = 0.814. By 2021, both achieved quality coordination: D of GBAUC = 0.990, D of YRDUC = 1. This study provides the first evidence from the sports industry that China’s new urbanization model and Document No. 46 are highly effective for synergistic regional economic growth.</div

Synthesis of a Lanthanide Metal–Organic Framework and Its Fluorescent Detection for Phosphate Group-Based Molecules Such as Adenosine Triphosphate

Adenosine triphosphate (ATP) is an important kind of metabolized biological molecule that is formed in organisms, especially in mitochondria, is used universally as energy, and is one of the most significant multifunctional biological molecules. Metal–organic frameworks (MOFs) have been widely used in many applications such as gas storage and separation, drug delivery, heterogeneous catalysis, chemical sensors, etc. Remarkably, lanthanide MOFs (Ln-MOFs), which display large pores, multiple dimensions, and unique lanthanide luminescence properties, are widely used as chemical sensors. A novel three-dimensional probe, Eu2(sbdc)3(H2O)3 (Eu-sbdc), was successfully self-assembled with Eu­(NO3)3·6H2O and 5,5-dioxo-5H-dibenzo­[b,d]­thiophene-3,7-dicarboxylic acid (H2sbdc). The Ln-MOF Eu-sbdc can quickly and effectively optically detect ATP via a luminescent quenching mechanism. The Ksv value of Eu-sbdc is 1.02 × 104 M–1, and the lower detection limit of Eu-sbdc for ATP is 20 μM, which is more sensitive to ATP. Its mechanism of monitoring ATP might be a dynamic or static quenching process. Eu-sbdc could effectively and quickly recognize ATP with high sensitivity

QD-Biopolymer-TSPP Assembly as Efficient BiFRET Sensor for Ratiometric and Visual Detection of Zinc Ion

In this work, we report a new type of quantum dot (QD)-based fluorescence resonance energy transfer (FRET) assembly and its utility for sensing Zn2+ in different media. The assembly on the QD scaffold is via first coating of poly­(dA) homopolymer/double-stranded DNA, followed by loading of meso-tetra­(4-sulfonatophenyl)­porphine dihydrochloride (TSPP), both of which are electrostatic, offering the advantages of cost-efficiency and simplicity. More importantly, the biopolymer coating minimizes the interfacial thickness to be ≤2 nm for QD-TSPP FRET, which results in improvements of up to 60-fold for single FRET efficiency and nearly 4-fold for total FRET efficiency of the QD-biopolymer-TSPP assemblies in comparison with silica-coating-based QD-TSPP assemblies. On the basis of Zn2+-chelation-induced spectral modulation, dual-emission QD-poly­(dA)-TSPP assemblies are developed as a ratiometric Zn2+ sensor with increased sensitivity and specificity. The sensor either in solution or on a paper substrate displays continuous color changes from yellow to bright green toward Zn2+, exhibiting excellent visualization capability. By utilizing the competitive displacement of Zn2+, the sensor is also demonstrated to have good reversibility. Furthermore, the sensor is successfully used to visualize exogenous Zn2+ in living cells. Together the QD-biopolymer-TSPP assembly provides an inexpensive, sensitive, and reliable sensing platform not only for on-site analytical applications but also for high-resolution cellular imaging

Liposomal Spherical Nucleic Acid Scaffolded Site-Selective Hybridization of Nanoparticles for Visual Detection of MicroRNAs

In this study, the advanced liposomal spherical nucleic acid (L-SNA) is exploited for the first time to establish a spherical, three-dimensional biosensing platform by hybridizing with a set of nanoparticles. By hydrophilic and hydrophobic interactions as well as programmable base-pairing, red-emission quantum dots (QDs), green-emission QDs, and gold nanoparticles (AuNPs) are encapsulated into the internal aqueous core, the intermediate lipid bilayer, and the outer SNA shell, respectively, producing an L-SNA–nanoparticle hybrid. As a result of the site-selective encapsulation, the hybrid constitutes a liposomal fluorescent “core–resonance energy transfer” system surrounded by a SNA shell, as is imaged at the single-particle resolution by confocal microscopy. With the outer SNA shell as three-dimensional substrate for duplex-specific nuclease target recycling reaction, the hybrid is capable of amplified detection of microRNAs, featuring one target to many AuNP-manipulated, dual-emission QD-based ratiometric fluorescence. More importantly, the ratiometric fluorescence facilitates the hybrid to visualize microRNAs with remarkably high resolution, which is exemplified by traffic light-type transition in fluorescence color for diagnosing circulating microRNAs in clinical serum samples. Substantially, the controllable hybridization with functional nanoparticles opens an avenue for the exciting biomedical applications of liposomal spherical nucleic acids