Additional file 2 of RNF38 suppress growth and metastasis via ubiquitination of ACTN4 in nasopharyngeal carcinoma

Additional file 2: Figure S2. Gene Ontology (GO) analysis (A) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis (B) of significantly enriched proteins in RNF38-overexpressing CNE-2 cells

Additional file 1 of RNF38 suppress growth and metastasis via ubiquitination of ACTN4 in nasopharyngeal carcinoma

Additional file 1: Figure S1. Gene Ontology (GO) analysis (A) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis (B) of significantly enriched proteins in RNF38-overexpressing SUNE-1 cells

Additional file 3 of RNF38 suppress growth and metastasis via ubiquitination of ACTN4 in nasopharyngeal carcinoma

Additional file 3: Supplementary Table S1. Comparison of RNF38 expression between nasopharyngeal carcinoma and normal nasopharyngeal epithelia tissues

Additional file 6 of RNF38 suppress growth and metastasis via ubiquitination of ACTN4 in nasopharyngeal carcinoma

Additional file 6: Supplementary Table S4. Proteins that specially enriched by RNF38 in SUNE-1 and CEN-2 cells. (XLS 67 kb

Additional file 4 of RNF38 suppress growth and metastasis via ubiquitination of ACTN4 in nasopharyngeal carcinoma

Additional file 4: Supplementary Table S2. Immunohistochemistry score of RNF38 expression in nasopharyngeal carcinoma and normal nasopharyngeal epithelia tissues

Additional file 5 of RNF38 suppress growth and metastasis via ubiquitination of ACTN4 in nasopharyngeal carcinoma

Additional file 5: Supplementary Table S3. Characteristics of patients with nasopharyngeal carcinoma grouped by RNF38 expression

DataSheet_1_Interleukin-15 and chemokine ligand 19 enhance cytotoxic effects of chimeric antigen receptor T cells using zebrafish xenograft model of gastric cancer.docx

Chimeric antigen receptor (CAR) T cells have been proven effective for the treatment of B-cell-mediated malignancies. Currently, the development of efficient tools that supply CAR T cells for the treatment of other malignancies would have great impact. In this study, interleukin (IL)-15 and C-C motif chemokine ligand 19 (CCL19) were introduced into natural killer group 2D (NKG2D)-based CARs to generate 15×19 CAR T cells, which remarkably increased T-cell expansion and promoted the production of central memory T (Tcm) cells. 15×19 CAR T cells showed greater cytotoxicity to gastric cell lines than conventional CAR T cells and produced higher levels of IL-15 and CCL-19, which resulted in increased responder T cell chemotaxis and reduced expression of T cell exhaustion markers. A live zebrafish model was used for single-cell visualization of local cytotoxicity and metastatic cancers. Administration of 15×19 CAR T cells resulted in significant shrinking of gastric cancer xenograft tumors and expansion of 15×19 CAR T cells in zebrafish models. Taken together, these findings demonstrate that 15×19 CAR T cells are highly efficient in killing gastric cancer cells, are effective to avoid off-target effects, and migrate to local and metastatic sites for long-term surveillance of cancers.</p

Additional file 1: of EBV-miR-BART8-3p induces epithelial-mesenchymal transition and promotes metastasis of nasopharyngeal carcinoma cells through activating NF-κB and Erk1/2 pathways

Table S1. miRNAs differentialy expressed in NPC versus control. Table S2. The top 20 most significantly up-regulated microRNAs in NPC. Table S3. Module assignment for microRNAs. Table S4. Enrichement of differentially expressed microRNAs in the coexpressed microRNA module. Table S5. Differerentially expressed genes in NPC versus control (NNPC). Table S6. Gene ontology and pathways enriched in the up- and down-regulated genes. The functional annotation gene sets were from the MSigDB database. FE, fold enrichment. Pvalue, P value was computed from Fisher’s exact test. P.adj, adjusted P value computed with Benjamini-Hochberg’s false discovery rate approach. Table S7. Enrichement of the differentially expressed gene signature in the microRNA-correlated gene signatures. (XLSX 732 kb

Additional file 2: of EBV-miR-BART8-3p induces epithelial-mesenchymal transition and promotes metastasis of nasopharyngeal carcinoma cells through activating NF-κB and Erk1/2 pathways

Figure S1. Gene ontology terms enriched in the up- and down-regulated gene signatures. The genes up-regulated in NPC are involved in cell cycle, neurogenesis and cell junction activities while those down-regulated are associated with immune response, suggesting activated cell proliferation and mitosis but inhibited immune defense in NPC. Figure S2. Upregulation of EBV-miR-BART8-3p shows no clear-cut effects on NPC cell proliferation in vitro. a, the effect of EBV-miR-BART8-3p on NPC CNE-1 and SUNE-1 cell proliferation is examined by CCK-8 assay; b, representative pictures (left panel) and quantification (left panel) of the colony-forming assays in CNE-1 and SUNE-1 cells. NS, no significant. Data are presented as mean ± SD. (DOCX 957 kb