Additional file 1: Figure S1. of The prognosis of invasive micropapillary carcinoma compared with invasive ductal carcinoma in the breast: a meta-analysis

(A) Funnel plot to detect publication bias for overall survival (OS). (B) Funnel plot to detect publication bias for disease-specific survival (DSS). (TIFF 304 kb

Additional file 2: Figure S2. of The prognosis of invasive micropapillary carcinoma compared with invasive ductal carcinoma in the breast: a meta-analysis

(A) Funnel plot to detect publication bias for relapse-free survival (RFS). (B) Funnel plot to detect publication bias for local-regional recurrence-free survival (LRRFS). (C) Funnel plot to detect publication bias for distant metastasis-free survival (DMFS). (TIFF 261 kb

Effect of Iron(II) on Arsenic Sequestration by δ‑MnO₂: Desorption Studies Using Stirred-Flow Experiments and X‑Ray Absorption Fine-Structure Spectroscopy

Arsenic (As) mobility in the environment is greatly affected by its oxidation state and the degree to which it is sorbed on metal oxide surfaces. Manganese (Mn) and iron (Fe) oxides are ubiquitous solids in terrestrial systems and have high sorptive capacities for many trace metals, including As. Although numerous studies have studied the effects of As adsorption and desorption onto Fe and Mn oxides individually, the fate of As within mixed systems representative of natural environments has not been resolved. In this research, As­(III) was initially reacted with a poorly crystalline phyllomanganate (δ-MnO₂) in the presence of Fe­(II) prior to desorption. This initial reaction resulted in the sorption of both As­(III) and As­(V) on mixed Fe/Mn-oxides surfaces. A desorption study was carried out using two environmentally significant ions, phosphate (PO₄^3–) and calcium (Ca²⁺). Both a stirred-flow technique and X-ray absorption fine-structure spectroscopy (XAFS) analysis were used to investigate As desorption behavior. Results showed that when As­(III)/Fe­(II) = 1:1 in the initial reaction, only As­(V) was desorbed, agreeing with a previous study showing that As­(III) is not associated with the Fe/Mn-oxides. When As­(III)/Fe­(II) = 1:10 in the initial reaction, both As­(III) and As­(V) can be desorbed from the Fe/Mn-oxide surface, and more As­(III) is desorbed than As­(V). Neither of the desorbents used in this study completely removed As­(III) or As­(V) from the Fe/Mn-oxides surface. However, the As desorption fraction decreases with increasing Fe­(II) concentration in the initial reactions

Image_3_Regional intra-arterial vs. systemic chemotherapy for the treatment of advanced pancreatic cancer: a systematic review and meta-analysis.tiff

IntroductionPancreatic cancer is a highly aggressive malignancy with limited response to chemotherapy. This research aims to compare the effectiveness and safety of regional intra-arterial chemotherapy (RIAC) with conventional systemic chemotherapy in treating advanced stages of pancreatic cancer.MethodsA comprehensive literature review was conducted using databases such as PubMed, Embase, Web of Science, and the Cochrane Library. Studies assessing the comparative outcomes of RIAC and systemic chemotherapy were included. Data extraction and quality evaluation were performed independently by two researchers. Statistical analysis was conducted using STATA16 software, calculating odds ratios (OR), risk differences (RD), and 95% confidence intervals (CI).ResultsEleven studies, comprising a total of 627 patients, were included in the meta-analysis. The findings showed that patients undergoing RIAC had significantly higher rates of partial remission (PR) compared to those receiving systemic chemotherapy (OR = 2.23, 95% CI: 1.57, 3.15, I2= 0%). Additionally, the rate of complications was lower in the RIAC group (OR = 0.45, 95% CI: 0.33, 0.63, I2= 0%). Moreover, patients treated with RIAC had notably longer median survival times.DiscussionThe results of this research indicate that RIAC is associated with a higher rate of partial remission, improved clinical benefits, and fewer complications compared to systemic chemotherapy in the management of advanced pancreatic cancer. These findings suggest that RIAC may be a more effective and safer treatment option for patients with advanced stages of pancreatic cancer.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42023404637.</p

Image_1_Regional intra-arterial vs. systemic chemotherapy for the treatment of advanced pancreatic cancer: a systematic review and meta-analysis.tiff

IntroductionPancreatic cancer is a highly aggressive malignancy with limited response to chemotherapy. This research aims to compare the effectiveness and safety of regional intra-arterial chemotherapy (RIAC) with conventional systemic chemotherapy in treating advanced stages of pancreatic cancer.MethodsA comprehensive literature review was conducted using databases such as PubMed, Embase, Web of Science, and the Cochrane Library. Studies assessing the comparative outcomes of RIAC and systemic chemotherapy were included. Data extraction and quality evaluation were performed independently by two researchers. Statistical analysis was conducted using STATA16 software, calculating odds ratios (OR), risk differences (RD), and 95% confidence intervals (CI).ResultsEleven studies, comprising a total of 627 patients, were included in the meta-analysis. The findings showed that patients undergoing RIAC had significantly higher rates of partial remission (PR) compared to those receiving systemic chemotherapy (OR = 2.23, 95% CI: 1.57, 3.15, I2= 0%). Additionally, the rate of complications was lower in the RIAC group (OR = 0.45, 95% CI: 0.33, 0.63, I2= 0%). Moreover, patients treated with RIAC had notably longer median survival times.DiscussionThe results of this research indicate that RIAC is associated with a higher rate of partial remission, improved clinical benefits, and fewer complications compared to systemic chemotherapy in the management of advanced pancreatic cancer. These findings suggest that RIAC may be a more effective and safer treatment option for patients with advanced stages of pancreatic cancer.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42023404637.</p

Image_2_Regional intra-arterial vs. systemic chemotherapy for the treatment of advanced pancreatic cancer: a systematic review and meta-analysis.tiff

IntroductionPancreatic cancer is a highly aggressive malignancy with limited response to chemotherapy. This research aims to compare the effectiveness and safety of regional intra-arterial chemotherapy (RIAC) with conventional systemic chemotherapy in treating advanced stages of pancreatic cancer.MethodsA comprehensive literature review was conducted using databases such as PubMed, Embase, Web of Science, and the Cochrane Library. Studies assessing the comparative outcomes of RIAC and systemic chemotherapy were included. Data extraction and quality evaluation were performed independently by two researchers. Statistical analysis was conducted using STATA16 software, calculating odds ratios (OR), risk differences (RD), and 95% confidence intervals (CI).ResultsEleven studies, comprising a total of 627 patients, were included in the meta-analysis. The findings showed that patients undergoing RIAC had significantly higher rates of partial remission (PR) compared to those receiving systemic chemotherapy (OR = 2.23, 95% CI: 1.57, 3.15, I2= 0%). Additionally, the rate of complications was lower in the RIAC group (OR = 0.45, 95% CI: 0.33, 0.63, I2= 0%). Moreover, patients treated with RIAC had notably longer median survival times.DiscussionThe results of this research indicate that RIAC is associated with a higher rate of partial remission, improved clinical benefits, and fewer complications compared to systemic chemotherapy in the management of advanced pancreatic cancer. These findings suggest that RIAC may be a more effective and safer treatment option for patients with advanced stages of pancreatic cancer.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42023404637.</p

Static Micromixer–Coaxial Electrospray Synthesis of Theranostic Lipoplexes

Theranostic lipoplexes are an integrated nanotherapeutic system with diagnostic imaging capability and therapeutic functions. They hold great promise to improve current cancer treatments; however, producing uniform theranostic lipoplexes with multiple components in a reproducible manner is a highly challenging task. Conventional methods, such as bulk mixing, are not able to achieve this goal because of their macroscale and random nature. Here we report a novel technique, called the static micromixer–coaxial electrospray (MCE), to synthesize theranostic lipoplexes in a single step with high reproducibility. In this work, quantum dots (QD605) and Cy5-labeled antisense oligodeoxynucleotides (Cy5-G3139) were chosen as the model imaging reagent and therapeutic drug, respectively. Compared with bulk mixing, QD605/Cy5-G3139-loaded lipoplexes produced by MCE were highly uniform with polydispersity of 0.024 ± 0.006 and mean diameter by volume of 194 ± 15 nm. MCE also showed higher encapsulation efficiency of QD605 and Cy5-G3139. QD605 and Cy5 also formed the Förster resonance energy transfer pair, and thus the cellular uptake and intracellular fate of theranostic lipoplexes could be visualized by flow cytometry and confocal microscopy. The lipoplexes were efficiently delivered to A549 cells (non-small cell lung cancer cell line) and down-regulated the Bcl-2 gene expression by 48 ± 6%

Characteristics of homology search of ‘Hangbaishao’ unigenes against the NR database.

<p><b>(A)</b> Venn diagram of number of unigenes annotated by BLASTx with an E-value threshold of 10^-5 against protein databases. The numbers in the circles indicate the number of unigenes annotated by single or multiple databases. <b>(B)</b> E-value distribution of the top BLASTx hits for each unigene. <b>(C)</b> Similarity distribution of the top BLASTx hits for each unigene. <b>(D)</b> Species distribution of the top BLASTx hits for all homologous sequences.</p

Transcriptomic Analysis of the Underground Renewal Buds during Dormancy Transition and Release in ‘Hangbaishao’ Peony (<i>Paeonia lactiflora</i>)

<div><p><i>Paeonia lactiflora</i> is one of the most famous species of herbaceous peonies with gorgeous flowers. Bud dormancy is a crucial developmental process that allows <i>P</i>. <i>lactiflora</i> to survive unfavorable environmental conditions. However, little information is available on the molecular mechanism of the bud dormancy in <i>P</i>. <i>lactiflora</i>. We performed de novo transcriptome sequencing using the Illumina RNA sequencing platform for the underground renewal buds of <i>P</i>. <i>lactiflora</i> ‘Hangbaishao’ to study the molecular mechanism underlying its bud dormancy transition (the period from endodormancy to ecodormancy) and release (the period from ecodormancy to bud elongation and sprouting). Approximately 300 million high-quality clean reads were generated and assembled into 207,827 (mean length = 828 bp) and 51,481 (mean length = 1250 bp) unigenes using two assembly methods named “Trinity” and “Trinity+PRICE”, respectively. Based on the data obtained by the latter method, 32,316 unigenes were annotated by BLAST against various databases. Approximately 1,251 putative transcription factors were obtained, of which the largest number of unique transcripts belonged to the basic helix-loop-helix protein (bHLH) transcription factor family, and five of the top ten highly expressed transcripts were annotated as dehydrin (DHN). A total of 17,705 simple sequence repeat (SSR) motifs distributed in 13,797 sequences were obtained. The budbreak morphology, levels of indole-3-acetic acid (IAA) and abscisic acid (ABA), and activities of guaiacol peroxidase (POD) and catalase (CAT) were observed. The expression of 20 interested unigenes, which annotated as DHN, heat shock protein (HSP), histone, late elongated hypocotyl (LHY), and phytochrome (PHY), and so on, were also analyzed. These studies were based on morphological, physiological, biochemical, and molecular levels and provide comprehensive insight into the mechanism of dormancy transition and release in <i>P</i>. <i>lactiflora</i>. Transcriptome dataset can be highly valuable for future investigation on gene expression networks in <i>P</i>. <i>lactiflora</i> as well as research on dormancy in other non-model perennial horticultural crops of commercial significance.</p></div

DEGs and main physiologcal or biochemical metabolisms identified by KEGG pathway annotation during five adjacent dormant phases.

<p>DEGs and main physiologcal or biochemical metabolisms identified by KEGG pathway annotation during five adjacent dormant phases.</p