41 research outputs found
Efficient quantification of the phenolic profiles of <i>Zanthoxylum bungeanum</i> leaves and correlation between chromatographic fingerprint and antioxidant activity
<p>Sixteen subsequent fractions were prepared from the ethyl acetate fraction of <i>Zanthoxylum bungeanum</i> leaves after bio-guided chromatographic separation. The HPLC profiles and antioxidant activity of the various fractions indicated that the content of eight phenolic compounds (chlorogenic acid, epicatechin, rutin, hyperoside, trifolin, quercitrin, afzelin and quercetin) and antioxidant activity vary significantly, and high concentrations of a combination of eight phenolic compounds would result in an increase of the antioxidant activity. These results suggested that the eight compounds could be used as chemical markers for quality assessment of <i>Z. bungeanum</i> leaves. Correlation between chromatographic fingerprint and antioxidant activity of the fractions showed that quercitrin and hyperoside play crucial roles in the antioxidant activity, and they can be seen as the milestone for quality control. The findings also suggested that five obtained fractions (E-3-3, E-2-4, E-7, E-5 and E-4) could become useful supplements for functional food ingredients and health-related products.</p
Reducing power of isolated compounds (mean ± SD, N = 3).
<p>Bars with no letters in common are significantly different (P<0.05).</p
<sup>1</sup>H, <sup>13</sup>C, HMBC and COSY NMR spectroscopic data (500 MHz, DMSO-d<sub>6</sub>) of compounds 1, 3, 5, 7 and 9.
<p>Assignments were done by 1D (<sup>1</sup>H, <sup>13</sup>C, DEPT) and 2D (COSY, HSQC, HMBC) NMR experiments.</p><p><sup>1</sup>H, <sup>13</sup>C, HMBC and COSY NMR spectroscopic data (500 MHz, DMSO-d<sub>6</sub>) of compounds 1, 3, 5, 7 and 9.</p
IC<sub>50</sub> values of isolated compounds for lipid peroxidation inhibition activity (mean ± SD, N = 3).
<p>Bars with no letters in common are significantly different (P<0.05).</p
ABTS·<sup>+</sup> radical scavenging activity of isolated compounds (mean ± SD, N = 3).
<p>Bars with no letters in common are significantly different (P<0.05).</p
Antioxidant activities of the extracts and different fractions from <i>Z. bungeanum</i> leaves.
<p>ECE: ethanol crude extracts; PEF: petroleum ether fraction; CF: chloroform fraction; EAF: ethyl acetate fraction; AF: acetone fraction; MF: methanol fraction.</p><p>Each values represented in tables are means ± SD (N = 3).</p><p>Values with different letters (a, b, c, d, e) within same column were significantly different (P<0.05).</p><p>Antioxidant activities of the extracts and different fractions from <i>Z. bungeanum</i> leaves.</p
The relative protective effect of isolated compounds on <i>E. coli</i> under peroxide stress: t = 30 min, the specific growth rate of <i>E. coli</i> in medium containing compounds and 6.5 mM H<sub>2</sub>O<sub>2</sub>/the specific growth rate in medium containing only 6.5 mM H<sub>2</sub>O<sub>2</sub> (the control) (mean ± SD, N = 3).
<p>Bars with no letters in common are significantly different (P<0.05).</p
<sup>1</sup>H, <sup>13</sup>C, HMBC and COSY NMR spectroscopic data (500 MHz, DMSO-d<sub>6</sub>) of compounds 2, 4, 6 and 8.
<p>Assignments were done by 1D (<sup>1</sup>H, <sup>13</sup>C, DEPT) and 2D (COSY, HSQC, HMBC) NMR experiments.</p><p><sup>1</sup>H, <sup>13</sup>C, HMBC and COSY NMR spectroscopic data (500 MHz, DMSO-d<sub>6</sub>) of compounds 2, 4, 6 and 8.</p
IC<sub>50</sub> values of isolated compounds for DPPH radical scavenging activity (mean ± SD, n = 3).
<p>Bars with no letters in common are significantly different (P<0.05).</p