6 research outputs found

    Survival and engraftment of transplanted ADSCs.

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    <p>(A) Representative images of in vivo BLI at days 1, 7, 14 and 28 (n = 12/group). The BLI signal decreased gradually from day 1 to day 28 after cells injection in both groups. But the signals in the Ex-ADSCs group were significantly higher than those in the ADSCs group. (B) Quantitative analysis of serial BLI signal showed a moderate signal was still observed in the Ex-ADSCs group at day 28 after transplantation. (C) Confocal laser microscopic images of ADSCs (fluc-mRFP), cardiomyocytes (cTnI, red florescence) and DAPI (blue fluorescence) at 2 weeks after transplantation (n = 4/group). Scale bars = 50 µm (D) Quantitative analysis of the ratio of fluc and mRFP double-positive cells. *<i>p</i><0.05.</p

    Confocal laser microscopic images of immunofluorescence analysis of differentiation of transplanted ADSCs in vivo (n = 12/group).

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    <p>(A and B) Representative images of differentiated cardiomyocytes-like cells using anti-cardiac troponin-I (green, cTnI) revealed significant augmentation of enhanced mRFP (red)/cTnI double positive cardiomyocyte-like cells (white arrow) in Ex-ADSCs group (B) compared with ADSCs group (A). (C) Quantitative analysis of the ratio of differentiated cardiomyocytes-like cells. (D and E) Representative images of differentiated vessel specific cells using anti-α-SMA (green) revealed significant enhancement of mRFP (red)/α-SMA double positive vessel-specific cells (white arrow) in Ex-ADSCs group (E) compared with ADSCs group(D). (F) Quantitative analysis of the ratio of differentiated vessel specific cells. *<i>p</i><0.05. Inset shows the corresponding boxed area magnified.</p

    Exendin-4 restores ROS-induced attenuation of ADSCs adhesion, increases their viability and promotes ADSCs proliferation at 30, 60, 120 min after pretreatment.

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    <p>(A) The effect of Exendin-4 on ADSCs adhesion in the presence of H<sub>2</sub>O<sub>2</sub>. Scale bars = 200 µm. (B) The effects of Exendin-4 on scavenging intracellular ROS of ADSCs which were treated with H<sub>2</sub>O<sub>2</sub> with or without Exendin-4. ROS of ADSCs were detected using DHE reagent. Scale bars = 100 µm. (C) Live/dead staining showed that the effects of Exendin-4 pretreatment on ADSCs viability against H<sub>2</sub>O<sub>2</sub>. Scale bars = 100 µm. (D) Quantification of adhesive ADSCs. (E) Quantification of intracellular ROS. (F) Quantification of viable ADSCs. (G) Quantitative analysis of LDH release in the cell supernatant. (H) Caspase-3 activity determined by using Caspase-3 ELISA kit. (I) MTT assay was performed to analyze the effect of Exendin-4 on viability of ADSCs after H<sub>2</sub>O<sub>2</sub> injury for 6 h. Statistical differences (<i>p</i><0.05) are indicated from Control group(*) and ADSCs+ H<sub>2</sub>O<sub>2</sub> group (#).</p

    Transplantation of Exendin-4 pretreated ADSCs decrease apoptosis, fibrosis, and promote angiogenesis.

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    <p>(A) Representative TUNEL staining images and quantitative analysis in the peri-infarcted and remote zone of heart sections from each group (n = 4/group). Apoptotic nuclei were identified as TUNEL positive (green). Total nuclei were counterstained by DAPI (blue). Apoptotic cells nuclei were considered as apoptotic cardiomyocytes (white arrow). Scale bars = 50 µm. (B) Representative images and quantitative analysis of fibrotic area in different groups by Masson's trichrome staining (n = 12/group). Red represented viable myocardium, blue represented fibrosis. Scale bars = 200 µm. (C) Representative images and quantitative analysis of vessels intensity using anti-vWF antibody at the border zone of MI in each group by immunohistochemistry (n = 12/group). Scale bars = 100 µm. Statistical differences (<i>p</i><0.05) are indicated from PBS (*) and ADSCs (#).</p
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