DataSheet_1_A mitochondria-related signature for predicting immune microenvironment and therapeutic response in osteosarcoma.docx

BackgroundOsteosarcoma remains to be the most devastating malignant tumor in children and teenagers. Mitochondria have also been proven to play critical roles in osteosarcoma. However, a mitochondria-related signature has been established in osteosarcoma to comprehensively evaluate the pathogenic roles and regulatory roles of mitochondria in osteosarcoma.MethodsIn this study, osteosarcoma samples' transcriptome data and clinical information were collected from Therapeutically Applicable Research to Generate Effective Treatments (TARGET) and Gene Expression Omnibus (GEO) databases. A comprehensive bioinformatics analysis was performed on the samples at the bulk RNA sequencing level and single-cell RNA sequencing (scRNA-seq) level. EdU, Transwell, and immunohistochemistry (IHC) were performed on PCCB.ResultsA mitochondria-related signature was constructed in osteosarcoma patients. The prognostic value of the mitochondria-related signature was explored. The predictive value of the mitochondria-related signature in the immune microenvironment and chemotherapy agents was explored. The association between mitochondria and immunity in the tumor microenvironment of osteosarcoma at the scRNA-seq level was investigated. The tumorigenic role of the critical mitochondria-related gene, PCCB, was verified by in vitro validation.ConclusionIn conclusion, a mitochondria-related signature was developed in osteosarcoma with solid predictive values in the immune microenvironment, chemotherapy agents, and prognosis.</p

Kaplan-Meier curves for NSCLC patients with COP-NLR = 2.

<p>(A) Kaplan-Meier curve of DFS for COP-NLR = 2 NSCLC patients. (B) Kaplan-Meier curve of OS for COP-NLR = 2 NSCLC patients.</p

Kaplan-Meier curves for NSCLC patients with different therapies.

<p>(A) Kaplan-Meier curve of DFS for surgery alone. (B) Kaplan-Meier curve of OS for surgery alone. (C) Kaplan-Meier curve of DFS for surgery and adjuvant chemotherapy. (D) Kaplan-Meier curve of OS for surgery and adjuvant chemotherapy.</p

Univariate analysis of DFS and OS for adenocarcinoma patients.

<p>Abbreviations: DFS = disease-free survival; OS = overall survival; HR = hazard ratio; CI = confidence interval; WBC = white blood cell; Hb = haemoglobin; LDH = lactate dehydrogenase; NLR = neutrophil/lymphocyte ratio; COP-NLR = combination of preoperative platelet count and neutrophil-lymphocyte ratio.</p><p>Univariate analysis of DFS and OS for adenocarcinoma patients.</p

Kaplan-Meier curves for 1238 NSCLC patients.

<p>(A) Kaplan-Meier curve of DFS for NSCLC. (B) Kaplan-Meier curve of OS for NSCLC.</p

Multivariate analysis of DFS and OS for all NSCLC patients.

<p>Abbreviations: DFS = disease-free survival; OS = overall survival; HR = hazard ratio; CI = confidence interval; WBC = white blood cell; Hb = hemoglobin; LDH = lactate dehydrogenase; COP-NLR = combination of preoperative platelet count and neutrophil-lymphocyte ratio.</p><p>Multivariate analysis of DFS and OS for all NSCLC patients.</p

Kaplan-Meier curves of stage I, II and IIIA NSCLC patients.

<p>(A) Kaplan-Meier curve of DFS for stage I. (B) Kaplan-Meier curve of OS for stage I. (C) Kaplan-Meier curve of DFS for stage II. (D) Kaplan-Meier curve of OS for stage II. (E) Kaplan-Meier curve of DFS for stage IIIA. (F) Kaplan-Meier curve of OS for stage IIIA.</p

Correlations between COP-NLR and clinicolaboratory characteristics.

<p>Abbreviations: COP-NLR = combination of preoperative platelet count and neutrophil-lymphocyte ratio; WBC = white blood cell; Hb = hemoglobin; LDH = lactate dehydrogenase; NLR = neutrophil/lymphocyte ratio.</p><p>Correlations between COP-NLR and clinicolaboratory characteristics.</p

Kaplan-Meiercurves for SqCC patients andadenocarcinoma patients.

<p>(A) Kaplan-Meier curve of DFS for SqCC. (B) Kaplan-Meier curve of OS for SqCC. (C) Kaplan-Meier curve of DFS for adenocarcinoma. (D) Kaplan-Meier curve of OS for adenocarcinoma.</p

Univariate analysis of DFS and OS for SqCC patients.

<p>Abbreviations: DFS = disease-free survival; OS = overall survival; HR = hazard ratio; CI = confidence interval; WBC = white blood cell; Hb = hemoglobin; LDH = lactate dehydrogenase; NLR = neutrophil/lymphocyte ratio; COP-NLR = combination of preoperative platelet count and neutrophil-lymphocyte ratio.</p><p>Univariate analysis of DFS and OS for SqCC patients.</p