7 research outputs found
Response of F<sub>1</sub> progenies of Asian corn borer to Cry1F, Cry1Ab and Cry1Ac.
<p>Response of F<sub>1</sub> progenies of Asian corn borer to Cry1F, Cry1Ab and Cry1Ac.</p
Toxicity of 5 Bt toxins against the Asian corn borer strains ACB-BtS and ACB-FR.
<p>Toxicity of 5 Bt toxins against the Asian corn borer strains ACB-BtS and ACB-FR.</p
Effective of dominance (<i>h</i>) of resistance to Cry1F in Cry1F-selected Asian corn borer.
<p>Effective of dominance (<i>h</i>) of resistance to Cry1F in Cry1F-selected Asian corn borer.</p
Knockdown of Hif-1α inhibited induction of activation markers upon hypoxia treatment in HSC-T6 cells.
<p>HSC-T6 cells in 6-well plates were transfected with either 100 nM Hif-1α siRNA or nonspecific (NS) siRNA for 24h and then cultured in room air or in 1% oxygen for 48h. (A) Cells were collected and cell lysates were subjected to detect Hif-1α, vimentin, α-SMA and β-actin with western blot; Densitometric analysis was performed using pooled data from three such experiments. Data are mean ± SD. (B) Hif-1α; (C) vimentin; (D) α-SMA. * : <i>P</i><0.05, ** : <i>P</i><0.01 (RA: room air, KD: knock down)..</p
Expression of Hif-1α apparently increased in liver tissues of <i>Schistosoma</i><i>japonicum</i> infected mice.
<p>BALB/c female mice, 6–8 weeks old, were percutaneously infected with 25 cercariae of <i>Schistosoma</i><i>japonicum</i> through the shaved abdomen, sacrificed at 6 weeks post-infection and samples of liver were collected. The expression of Hif-1α and α-SMA in <i>Schistosoma</i><i>japonicum</i> infected (n=3) and non-infected (n=3) mice liver was detected with western blot and immunohistochemistry. (A) 1: protein extract from the liver of non-infected Balb/C mice, 2: protein extract from livers of <i>Schistosoma</i><i>japonicum</i> cercariae infected Balb/C mice. (B) Densitometric analysis (n=3). Data are mean ± SD. * : <i>P</i><0.05, ** : <i>P</i><0.01. (C) Acute inflammatory cell infiltration and granulomatous inflammation, surrounding eggs of <i>Schistosoma</i><i>japonicum</i>. (D) Expression of Hif-1α in non-infected mice liver. (E) Expression of Hif-1α in infected liver.</p
1% O<sub>2</sub> induced increased expression of vimentin and α-SMA in HSC-T6 cells.
<p>HSC-T6 cells were cultured in room air or in 1% oxygen. (A) Vimentin expression in HSC-T6 cells grown on the coverslips at 48 hours post-seeding were co-stained with anti-vimentin (red) and DAPI (blue) by immunocytochemistry. (B) Cells were collected at indicated time and cell lysates were subjected to detect vimentin and α-SMA. Densitometric analysis was performed using pooled data from three such experiments. Data are mean ± SD. (C) vimentin; (D) α-SMA. * : <i>P</i><0.05.</p
1% O<sub>2</sub> induced apparent accumulation of Hif-1α and reorganization of F-actin in HSC-T6 cells.
<p>HSC-T6 cells, rat hepatic stellate cell line, were cultured in room air or in 1% oxygen. (A) Cells were collected at indicated time and cell lysates were subjected to detect Hif-1α and β-actin with western blot. (B) F-actin was stained using Fluorescein Isothiocyanate (FITC)-labeled phalloidin to capture F-actin of cells grown on the coverslips.</p