Data_Sheet_1_OsWAK112, A Wall-Associated Kinase, Negatively Regulates Salt Stress Responses by Inhibiting Ethylene Production.pdf

The wall-associated kinase (WAK) multigene family plays critical roles in various cellular processes and stress responses in plants, however, whether WAKs are involved in salt tolerance is obscure. Herein, we report the functional characterization of a rice WAK, WAK112, whose expression is suppressed by salt. Overexpression of OsWAK112 in rice and heterologous expression of OsWAK112 in Arabidopsis significantly decreased plant survival under conditions of salt stress, while knocking down the OsWAK112 in rice increased plant survival under salt stress. OsWAK112 is universally expressed in plant and associated with cell wall. Meanwhile, in vitro kinase assays and salt tolerance analyses showed that OsWAK112 possesses kinase activity and that it plays a negative role in the response of plants to salt stress. In addition, OsWAK112 interacts with S-adenosyl-L-methionine synthetase (SAMS) 1/2/3, which catalyzes SAM synthesis from ATP and L-methionine, and promotes OsSAMS1 degradation under salt stress. Furthermore, in OsWAK112-overexpressing plants, there is a decreased SAMS content and a decreased ethylene content under salt stress. These results indicate that OsWAK112 negatively regulates plant salt responses by inhibiting ethylene production, possibly via direct binding with OsSAMS1/2/3.</p

Additional file 2 of EBF1-mediated up-regulation of lncRNA FGD5-AS1 facilitates osteosarcoma progression by regulating miR-124-3p/G3BP2 axis as a ceRNA

Additional file 2. Figure S2 (A–B) The proliferation of HOS cell line in different groups was verified through immunofluorescence as well as colony formation assays. (C-E) JC-1, together with TUNEL assay and caspase-3/8/9 assay was performed to evaluate cell apoptosis rate. (F) Transwell assays were carried out to measure cell invasion. (G) The binding situation of EBF1 and FGA5-AS1 promoter in HOS cells was measured by DNA pull down assay. (H) TCGA database result of EBF1 expression in sarcoma tissue samples compared with normal tissue samples. *P < 0.05, **P < 0.01, n.s.: no significanc

Additional file 3 of EBF1-mediated up-regulation of lncRNA FGD5-AS1 facilitates osteosarcoma progression by regulating miR-124-3p/G3BP2 axis as a ceRNA

Additional file 3. Figure S3 (A) UCSC and PROMO were taken to predict the upstream potential transcription factor of FGD5-AS

Additional file 1 of EBF1-mediated up-regulation of lncRNA FGD5-AS1 facilitates osteosarcoma progression by regulating miR-124-3p/G3BP2 axis as a ceRNA

Additional file 1. Figure S1 (A) The possible miRNAs were predicted by miRmap, TargetScan, microT, miRanda and PicTar database in starBase. (B) TCGA database was applied to detect the expression of FGA5-AS1 in sarcoma tissue samples compared with normal tissue samples. *P < 0.0

Additional file 3 of Genome-wide identification, characterization and transcriptional profile of the SWEET gene family in Dendrobium officinale

Supplementary Material

<i>In vitro</i> and <i>in vivo</i> activity of meropenem+avibactam against MBL-producing carbapenem-resistant <i>Klebsiella pneumoniae</i>

Antibiotic resistance has become a public health problem to be solved worldwide and metallo-β-lactamase (MBL)-producing bacteria make this problem even more challenging. The interactions of meropenem (MEM) in combination with avibactam (AVI) in growth inhibition on MBL-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) strains were tested. In vitro interactions of MEM+AVI were tested using the microdilution checkerboard assay and time–kill curves. In vivo interactions of MEM+AVI were tested using the Galleria mellonella model. All strains were multi-drug resistant strains and six of them were proved to produce MBLs. We show that the combination of MEM+AVI generates profound synergistic effects on growth inhibition of all strains, which was better than that of MEM+vaborbactam or imipenem+relebactam. The time-kill curves further confirmed the potent synergistic antibacterial effects of MEM+AVI against MBL-producing CRKP strains. Galleria mellonella studies were consistent with in vitro analysis. Combining MEM with AVI improved survival rates and mean survival days were obviously prolonged compared to the drug alone and the untreated controls. To our knowledge, this study is the first report of MEM+AVI collaborating against MBL-producing CRKP strains. Our findings showed that the combination of MEM+AVI has the potential for antibiotic drug development to combat MBL-producing pathogens.</p

Additional file 5 of Genome-wide identification, characterization and transcriptional profile of the SWEET gene family in Dendrobium officinale

Supplementary Material

Additional file 6 of Genome-wide identification, characterization and transcriptional profile of the SWEET gene family in Dendrobium officinale

Supplementary Material