sj-xlsx-1-tct-10.1177_15330338211045831 - Supplemental material for CBX2 Induces Glioma Cell Proliferation and Invasion Through the Akt/PI3K Pathway

Supplemental material, sj-xlsx-1-tct-10.1177_15330338211045831 for CBX2 Induces Glioma Cell Proliferation and Invasion Through the Akt/PI3K Pathway by Le Wang, Bingcheng Ren, Hao Zhuang, Yue Zhong and Yang Nan in Technology in Cancer Research & Treatment</p

Exploring Degradation of Mutant and Wild-Type Epidermal Growth Factor Receptors Induced by Proteolysis-Targeting Chimeras

Several epidermal growth factor receptor (EGFR) proteolysis-targeting chimeras (PROTACs), including MS39 and MS154 developed by us, have been reported to effectively degrade the mutant but not the wild-type (WT) EGFR. However, the mechanism underlying the selectivity in degrading the mutant over the WT EGFR has not been elucidated. Here, we report comprehensive structure–activity relationship studies that led to the discovery of two novel EGFR degraders, 31 (MS9449) and 72 (MS9427), and mechanistic studies of these EGFR degraders. Compounds 31 and 72 selectively degraded the mutant but not the WT EGFR through both ubiquitination/proteasome and autophagy/lysosome pathways. Interestingly, we found that the mutant but not the WT EGFR can effectively form EGFR–PROTAC–E3 ligase ternary complexes. Furthermore, we found that PI3K inhibition sensitized WT EGFR to PROTAC-induced degradation and combination treatment with a PI3K inhibitor enhanced antiproliferation activities of EGFR degraders in cancer cells harboring WT EGFR, providing a potential therapeutic strategy for patients with WT EGFR overexpression

Study on the bond properties between basalt fiber-reinforced spontaneous combustion coal gangue concrete and BFRP bars

Microstructural characterization of spontaneous combustion coal gangue (SCCG), the hydration products and mechanism of spontaneous combustion coal gangue concrete (SCCGC) were discerned through microscopic analysis. The bond performance was assessed employing a central pull-out test on samples variably substituted with SCCG (0%, 25%, 50%, 75%, and 100%) and augmented with basalt fiber (BF) (0%, 0.1%, 0.15%, and 0.2%). The failure mode and bonding mechanism were also revealed by this test. The bond-slip curves were fitted by various bond-slip constitutive models and a suitable model was found for each section. As indicated by the results, SCCGC possessed a lower carbon content and higher Al and Si element contents. These elements would undergo secondary hydration reactions with CH, which could enhance the strength of the ITZ and the compactness of the bond interface between BFRP bars and concrete. The failure modes were splitting and pull-out. An inverse correlation was observed between bond strength and the increment in SCCG aggregate substitution, ranging from a decline of 2.6% to 23.1%. As the BF content increased, the bond strength and peak slip increased by 3.9% ∼ 19.7% and 4.0% ∼ 14.6%, respectively. Furthermore, the reinforcing effect of BF on bond strength increased from 3.9% ∼ 10.3% to 8.8% ∼ 19.7% as the SCCG replacement rate increased, which was noticeable. The Malvar model and the Continuous Curve model were the best fitting models for the ascending and descending sections of bond-slip curves, respectively, while the residual stage was well fitted by the Hao Qingduo model.</p

Exploring Degradation of Mutant and Wild-Type Epidermal Growth Factor Receptors Induced by Proteolysis-Targeting Chimeras

Several epidermal growth factor receptor (EGFR) proteolysis-targeting chimeras (PROTACs), including MS39 and MS154 developed by us, have been reported to effectively degrade the mutant but not the wild-type (WT) EGFR. However, the mechanism underlying the selectivity in degrading the mutant over the WT EGFR has not been elucidated. Here, we report comprehensive structure–activity relationship studies that led to the discovery of two novel EGFR degraders, 31 (MS9449) and 72 (MS9427), and mechanistic studies of these EGFR degraders. Compounds 31 and 72 selectively degraded the mutant but not the WT EGFR through both ubiquitination/proteasome and autophagy/lysosome pathways. Interestingly, we found that the mutant but not the WT EGFR can effectively form EGFR–PROTAC–E3 ligase ternary complexes. Furthermore, we found that PI3K inhibition sensitized WT EGFR to PROTAC-induced degradation and combination treatment with a PI3K inhibitor enhanced antiproliferation activities of EGFR degraders in cancer cells harboring WT EGFR, providing a potential therapeutic strategy for patients with WT EGFR overexpression

Additional file 1 of Curcumin improves insulin sensitivity in high-fat diet-fed mice through gut microbiota

Additional file 1: Fig. S1. The average body weight of curcumin- and vehicle-treated mice before and after fed with HFD for 4 weeks (n = 8/group). Fig. S2. The average liver weight of curcumin- and vehicle-treated mice after fed with HFD for 4 weeks (n = 8/group). Fig. S3. The average body weight gain of curcumin- and vehicle-treated HFD-fed mice during endogenous gut microbiota depletion (n = 8/group). Fig. S4. The average body weight of endogenous gut microbiota-depleted HFD-fed mice before and after colonized with the microbiota harvested from curcumin- and vehicle-treated HFD-fed mice for 4 weeks (n = 8/group)

Datasheet1_A bibliometric analysis of research on pediatric preoperative anxiety (2007–2022).pdf

ObjectiveThis study aimed to analyze the current state of research on preoperative anxiety in children through CiteSpace, VOSviewer, and the identification of hot spots and frontiers.MethodRelevant data were retrieved from the Web of Science Core Collection using the search terms children and preoperative anxiety. Data were analyzed using VOSviewer (version 1.6.18), CiteSpace (5.7. R5) software, and Scimago Graphica.ResultsA total of 622 articles were published between 2007 and 2022, with an increasing trend over time. Kain, Zeev N. (13; 2.09%) and Dalhousie University (15; 2.41%) were the most influential authors and most prolific institutions, respectively. The United States (121; 19.45%) was the country with the most publications. Pediatric anesthesia (55; 8.84%) had the most publications. High-frequency keywords were categorized into three themes, including nonpharmacologic interventions for preoperative anxiety in children, preoperative medications, and risk factors for anxiety; of these, “predictor” (38; 2016) and “sedative premedication” (20; 2016) were the most studied keywords over the past 6 years. “Distraction” (67; 2019) and “dexmedetomidine” (65; 2019) have been the main areas of interest in recent years.ConclusionResearch on preoperative anxiety in children has been the focus of increasing attention over the past fifteen years, with the majority of publications from high-income countries. This review provides a useful perspective for understanding research trends, hot topics, and research gaps in this expanding field.</p

Highly Entangled Hydrogel Enables Stable Zinc Metal Batteries via Interfacial Confinement Effect

Hydrogel electrolytes are expected to be useful for stable zinc metal batteries; however, it is extremely challenging to develop a hydrogel electrolyte that balances mechanical properties, ionic conductivity, and interface stability. Herein, we report a highly entangled hydrogel electrolyte that achieves pronounced mechanical properties (446 kPa tensile strength) and high ionic conductivity (3.93 mS cm–1) by optimizing the dense reversible conformation of hydrophilic chains. Moreover, due to the interfacial confinement effect, the highly entangled hydrogel electrolyte enables a dynamically stabilized anode with a non-dendritic planar morphology. Thus, the zinc anode with this electrolyte system exhibits a long-term cycle stability of 6000 h at 0.5 mA cm–2 and over 220 h at an impressive depth-of-discharge of 68.4%. This work provides an important concept for realizing practical high-performance wearable zinc–manganese batteries

Table_1_Research Hotspots and Trends in Music Therapy Intervention for Patients With Dementia: A Bibliometrics and Visual Analysis of Papers Published From 2010 to 2021.pdf

BackgroundAs a serious public health problem, dementia has placed a heavy burden on society and families. Evidence suggests that the use of music therapy as a non-pharmacological intervention has certain advantages with respect to reducing the behavioral and psychological symptoms of dementia (BPSD) and improving the cognition and mental status of dementia patients. However, research trends and hotspots regarding music therapy intervention for dementia analysis have not been systematically studied via bibliometric analysis.MethodsWe searched the Web of Science Core Collection (WoSCC) for texts published between January 1, 2010, and October 31, 2021, and visualized country, institution, journal, keyword co-occurrence, keyword emergence and keyword clustering.ResultsA total of 217 articles from the WoSCC database were analyzed. In this research field, the annual number of publications has generally shown a slowly increasing trend, and the United States has the most publications and the most frequent cooperation among countries. University College London (UCL) has the most extensive influence among research institutions. Among articles, those published in the JOURNAL OF ALZHEIMER'S DISEASE were the most numerous, with 20 such articles being published, accounting for 9.22% (20/217) of the total. Comprehensive analysis of five clusters via biclustering shows that the research hotspots in this field during the past 11 years have mainly focused on the autobiographical memory, cognitive function, mental state and BPSD of dementia patients.ConclusionThis study conducted a bibliometric and visual analysis of relevant studies concerning music therapy intervention for dementia patients. Psychological problems faced by dementia patients and the topics of quality of life, individualized music therapy, the mental state of caregivers and other related topics may be important research directions in the future. Therefore, the question of how to develop standardized research protocols and identify unified efficacy evaluation indicators should be a focus of and difficulty for future research.</p

Hierarchical 1D/2D V₃S₄@N, S‑Codoped rGO Hybrids as High-Performance Anode Materials for Fast and Stable Lithium-Ion Storage

Vanadium sulfides, possessing high theoretical specific capacity and diverse structures, are regarded as attractive and promising anode materials for Li storage. Unfortunately, their practical applications are restricted by intrinsic drawbacks, like inferior structure stability, large volume variation, and even polysulfide dissolutions. Herein, we display a hierarchical one-dimensional/two-dimensional (1D/2D) hybrid material with 1D V3S4 nanofibers supported on 2D N, S-codoped graphene nanosheets and further unveil its electrochemical mechanism for Li-storage and origin of enhanced performance. When applied in LIBs, the optimized 1D/2D hybrid anodes deliver a stable capacity of 717 mA h g–1 over 90 cycles at 100 mA g–1, prolonged cycling stability over 1000 cycles at 1 A g–1, and an excellent rate capability of 370 mA g–1 at 5 A g–1, which are superior to those of the pure V3S4 anode. Such structural design and engineering may provide inspiration for exploring other nanocomposites to achieve desired electrochemical performance

Investigation of the Effects of Biodiesel Feedstock on the Performance and Emissions of a Single-Cylinder Diesel Engine

Biodiesel fuels may serve as a partial solution in the search for sustainable energy sources for the transportation sector. However, increased nitrogen oxide (NO_<i>x</i>) emissions are a potentially significant drawback to the use of biodiesel fuels that must be addressed if biodiesel is to gain widespread acceptance. One approach is to identify specific biodiesel fuel properties that minimize NO_<i>x</i> formation and use these to produce lower NO_<i>x</i> fuel blends. In this work, seven biodiesel fuels were produced from high-erucic rapeseed, olive, palm, coconut, soybean, and fresh and used canola oils, with their chemical composition determined using gas chromatography–mass spectrometry (GC–MS). The fuels were then burned in a single-cylinder direct-injection diesel engine and evaluated for both fuel consumption and exhaust emissions of nitrogen oxides, carbon monoxide (CO), unburned hydrocarbons, and particulate matter. While all biodiesels had higher brake-specific nitric oxide (NO) emissions than ultralow sulfur diesel (ULSD) at low engine loads, olive, palm, coconut, and canola biodiesels performed better than ULSD at 50% loading and above. Nitrogen dioxide (NO₂), CO, and unburned hydrocarbon emissions were generally lower from the biodiesel fuels than ULSD. Palm biodiesel consistently generated the lowest brake-specific NO_<i>x</i> levels of all tested fuels. Statistical analysis of the results showed that higher fuel hydrogen/carbon molar ratios, low polyunsaturation levels, and lower fuel density were all significantly associated with reduced NO emissions in the tested biodiesel fuels but no clear trends were observed for NO₂. The results suggest that pathways exist for tailoring the fuel properties of biodiesel blends to reduce nitrogen oxide emission compared to current fuels