Data_Sheet_1_Structural Characteristics and Functional Implications of PM2.5 Bacterial Communities During Fall in Beijing and Shanghai, China.docx

Air pollution characterized by fine particulate matter (PM2.5) frequently has occurred in China, and has posed threats to human health. The physiochemical characteristics of airborne PM2.5 have been extensively studied, but its bacterial structures and functions have not yet been well studied. Herein, we focused on the structural characteristics and functional implications of airborne bacteria under different pollution levels in Beijing and Shanghai. The α- and β-diversities showed no obvious difference in two cities (p > 0.05). The dominant phyla Proteobacteria, Firmicutes, and Actinobacteria with total abundance of over 92% were found in all PM2.5 samples. The results of weighted unifrac non-metric multidimensional scaling (NMDS) suggested that air pollution was no obviously correlated with bacterial community but dispersed disorderly. Furthermore, canonical correlation analysis (CCA) and permutation test indicated that NH4+, SO42-, and wind speed were the key factors that associated with airborne bacterial community structure. Chemical components of particulate matter played more important role in structuring bacterial community than meteorological conditions based on the result of partial CCA. In addition, the annotation of metabolic pathway suggested that the predominant genus Pseudomonas was obviously correlated with disease infections. Several dominant species might contribute to organic degradation, nitrogen cycles, and ice-nuclei activities in environments. Overall, this work enhanced our understanding of functions of airborne bacteria and highlighted their potential role in atmospheric chemical progresses.</p

MOESM1 of Metabolic engineering of Acremonium chrysogenum for improving cephalosporin C production independent of methionine stimulation

Additional file 1: Table S1. Strains and plasmids used in this study. Table S2. Primers used in this study. Fig. S1. Cephalosporin C production of WT detected by UPLC/MS in the MDFA medium with or without addition of 3.2 g/L methionine. Fig. S2. Mycelium dry weight of A. chrysogenum in the MDFA medium with or without addition of 3.2 g/L methionine. Fig. S3. Sequence alignment and phylogenetic analysis of the SAM synthetase family proteins. Fig. S4. Cephalosporin C production of WT and WT/pAg1PT-G418 in the MDFA medium with or without addition of 3.2 g/L methionine. Fig. S5. Construction and validation of the AcsamS overexpressed strain (AcsamsOE). Fig. S6. Cephalosporin C production of WT and AcsamsOE was detected by UPLC/MS in MDFA medium. Fig. S7. Mycelium dry weight of AcsamsOE in the MDFA medium with or without addition of 3.2 g/L methionine. Fig. S8. Cephalosporin C production of AcsamsOE in the MDFA medium supplemented with different concentration of SAM. Fig. S9. Sequence alignment of the leucine carboxyl methyltransferase superfamily proteins. Fig. S10. Construction and validation of the Acppm1 disruption mutant (Acppm1DM). Fig. S11. Cephalosporin C production of WT and Acppm1DM was detected by UPLC/MS. Fig. S12. Mycelium dry weight of Acppm1DM, Acppm1CM, Acppm1OE in the MDFA medium with or without addition of 0.32 g/L methionine. Fig. S13. The relative transcriptional level of AcsamS in WT and Acppm1DM. Fig. S14. The relative transcriptional level of AcmetH, AccysD, AcmecA and mecB of WT in the MDFA medium with or without addition of 3.2 g/L methionine. Fig. S15. Cephalosporin C production of Acppm1DM and Acppm1DM-AcsamsOE. Fig. S16. The relative transcriptional level of mecB in Acppm1DM. Fig. S17. Construction and validation of the mecB overexpressed strain (Acppm1DM-mecBOE). Fig. S18. Cephalosporin C production of WT and Acppm1DM-mecBOE was detected by UPLC/MS in MDFA medium. Fig. S19. Mycelium dry weight of WT and Acppm1DM-mecBOE in the MDFA medium supplemented with 0, 0.32 g/L and 3.2 g/L of methionine respectively. Fig. S20. Construction and validation of Acppm1DM-mecBOE-AcsamsOE. Fig. S21. Cephalosporin C production of Acppm1DM-mecBOE-AcsamsOE

3D face scan by using scanning APP.

Participant taking 3D face scan with scanning APP installed on a smartphone. Whole process cost less than 1 minute.</p

Fit factor test data.

M group: surgical masks without a retainer. MR, group of surgical masks with retainers. iNB, initial normal breathing. DB, deep breathing. Head L/R, head movement from side to side. Head U/D, head movement up and down. fNB, final normal breathing. (DOCX)</p

3D face scans acquired with face scanning APP and CAD of retainer.

Panel A shows anatomical facial landmarks used to determine border of retainer: A, rhinion; B, margo infraorbitalis; C, processus temporalis ossis zygomatici; D, vertical line at angulus oris plane; E, angulus oris. Panel B shows CAD of retainer merged on 3D face scan. Panel C shows CAD of retainer merged on 3D face scan with mask. CAD, computer aided design.</p

Subjective perceived discomfort.

Subjective perceived discomfort.</p

Characteristics of subjects.

Characteristics of subjects.</p

Scores of FF of surgical mask with and without retainer.

Scores of FF of surgical mask with and without retainer.</p

Copper-Catalyzed Synthesis of α-Amino Imides from Tertiary Amines: Ugi-Type Three-Component Assemblies Involving Direct Functionalization of sp³ C−Hs Adjacent to Nitrogen Atoms

α-Amino imides can be accessed straightforwardly from tertiary amines through copper-catalyzed three-component reactions involving the direct functionalization of sp3 C−Hs adjacent to nitrogen atoms. This reaction has demonstrated a tolerance to a wide range of functionalizations and can be performed under very mild conditions. A plausible mechanism has been proposed in which an Ugi-type cascade assembly has been included

Subjective ratings of discomfort in eight domains of 10 subjects.

M group: surgical masks without a retainer. MR, group of surgical masks with retainers. The participants were asked at 60, 80 and 100 min of the intermittent exercise how they perceived the comfort in the questionnaire. (DOCX)</p