Evaluation of protein expression (Western-blot).

<p>(<b>A</b>) Protein ratios were calculated relative to β-actin (loading control). <b>§</b>: <i>P</i><0.01 compared with SHO group, <b></b>: <i>P</i><0.01 compared with UUO group, <b>☆</b>: <i>P</i>>0.05 compared with UUO group. (<b>B</b>) Representative Western blot for PHB, TGF-β1, Col-I, Col-III, Col-IV, and FN in SHO (sham operation), UUO (model group subjected to unilateral ureteral obstruction), PHB⁻ (UUO rats treated with lentivirus carrying siRNA PHB), and PHB⁺ (UUO rats treated with lentivirus carrying PHB) groups; Negative control: UUO rats treated with control viruses.</p

mRNA expression of PHB and TGF-β1 in RIF.

<p><b>§</b>: <i>P</i><0.01 compared with SHO (sham operation) group, <b></b>: <i>P</i><0.01 compared with UUO group (model group subjected to unilateral ureteral obstruction), <b>☆</b>: <i>P</i>>0.05 compared with UUO group. PHB⁻ (UUO rats treated with lentivirus carrying siRNA PHB); PHB⁺ (UUO rats treated with lentivirus carrying PHB) groups; Negative control: UUO rats treated with control viruses.</p

Assessment of oxidative stress and antioxidative capacity.

<p>Changes in general reactive oxygen species (ROS) levels and malondialdehyde (MDA) generation, a final degradation product of lipid peroxidation, are shown in (<b>A</b>) and superoxide dismutase (SOD) and reduced glutathione (GSH) in (<b>B</b>). <b>§</b>: <i>P</i><0.01 compared with SHO group (sham operation), <b></b>: <i>P</i><0.01 compared with UUO group (model group subjected to unilateral ureteral obstruction), <b>☆</b>: <i>P</i>>0.05 compared with UUO group. PHB⁻: UUO rats treated with lentivirus carrying siRNA PHB; PHB⁺; UUO rats treated with lentivirus carrying PHB; Negative control: UUO rats treated with control viruses; AOX: antioxidant.</p

Meta analysis for the association of ACE I/D gene polymorphism with SSNS susceptibility.

<p>Meta analysis for the association of ACE I/D gene polymorphism with SSNS susceptibility.</p

Gene distribution characteristics of ACE I/D gene for SRNS and SSNS.

<p>Gene distribution characteristics of ACE I/D gene for SRNS and SSNS.</p

Association between D allele and SRNS susceptibility or SSNS risk.

<p>A: D allele and SRNS; B: D allele and SSNS.</p

Characteristics of the studies evaluating the effects of ACE I/D gene polymorphism on SSNS onset.

<p>Characteristics of the studies evaluating the effects of ACE I/D gene polymorphism on SSNS onset.</p

Meta analysis for the difference of the ACE I/D gene distribution between SRNS and SSNS.

<p>Meta analysis for the difference of the ACE I/D gene distribution between SRNS and SSNS.</p

Meta analysis for the association of ACE I/D gene polymorphism with SRNS risk.

<p>Meta analysis for the association of ACE I/D gene polymorphism with SRNS risk.</p

D allele or DD genotype distribution difference between SRNS and SSNS.

<p>A: D allele; B: DD genotype.</p