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Quantification of propidium iodide delivery with millisecond electric pulses: A model study
A model study of propidium iodide delivery with millisecond electric pulses
is presented; this work is a companion of the experimental efforts by Sadik et
al. [1]. Both membrane permeabilization and delivery are examined with respect
to six extra-cellular conductivities. The transmembrane potential of the
permeabilized regions exhibits a consistent value, which corresponds to a
bifurcation point in the pore-radius-potential relation. Both the pore area
density and membrane conductance increase with an increasing extra-cellular
conductivity. On the other hand, the inverse correlation between propidium
iodide delivery and extra-cellular conductivity as observed in the experiments
is quantitatively captured by the model. This agreement confirms that this
behavior is primarily mediated by electrophoretic transport during the pulse.
The results suggest that electrophoresis is important even for the delivery of
small molecules such as propidium iodide. The direct comparison between model
prediction and experimental data presented in this work helps validate the
former as a robust predictive tool for the study of electroporation
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