Additional file 1: Table S1. of Occurrence of Blastocystis sp. and Pentatrichomonas hominis in sheep and goats in China

Occurrence and subtypes distributions of B. hominis and P. hominis in sheeps and goats in China. (DOC 94 kb

Effects of boron on the proliferation, apoptosis and immune function of splenic lymphocytes through ERα and ERβ

This experiment aimed to investigate the role of ERα and ERβ in boron's effect on splenic lymphocyte of rat. After inhibition of ERα and ERβ using MPP and PHTPP, the effects of 0.4 mmol/L boron on the splenic CD4+/CD8+ T-cell ratio, lymphocyte proliferation rate, IFN-γ concentration and PCNA protein expression disappeared. Furthermore, after inhibiting ERβ with PHTPP alone, the decreasing effects of 40 mmol/L boron on the splenic CD8+ T-cell proportion, lymphocyte proliferation rate, the concentrations of IgG and cytokines, as well as the expression of Bcl-2 also disappeared. However, when ERα was inhibited by MPP alone, the effect of 40 mmol/L boron on splenic lymphocyte was unchanged. The results suggest that ERα mainly mediate the effects of low-dose boron (0.4 mmol/L) on splenic T-cell subsets, lymphocyte proliferation and apoptosis, and immune function, while ERβ may mediate the effects of both low-dose and high-dose boron (40 mmol/L) on splenic lymphocytes.</p

Additional file 1 of Establishment of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of swine acute diarrhea syndrome coronavirus (SADS-CoV)

Additional file 1: Supplementary Fig. 1. Stability test of SADS-CoV for five times replicate experiments using (A) 105 copies/μL and (B) 103 copies/μL concentration standards, respectively

Additional file 2 of Establishment of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of swine acute diarrhea syndrome coronavirus (SADS-CoV)

Additional file 2: Supplementary Fig. 2. (A-C) Repeatability test of SADS-CoV for five times replicate experiments using three clinical virus-infected samples, respectively

Additional file 3 of Establishment of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of swine acute diarrhea syndrome coronavirus (SADS-CoV)

Additional file 3: Supplementary Table 1. The detection results of 11 samples by quantitative real-time PCR assays and the RT-PCR system

Additional file 1 of Study of microencapsulated fatty acid antimicrobial activity in vitro and its prevention ability of Clostridium perfringens induced necrotic enteritis in broiler chicken

Additional file 1: Table S1. Ingredients and composition of wheat-based die