Additional file 3: of Hot water extract of Agaricus blazei Murrill specifically inhibits growth and induces apoptosis in human pancreatic cancer cells

Tables S3. Genes significantly overrepresented by PANTHER Overrepresentation Test based on Gene Ontology (GO) data. (XLS 108 kb

Additional file 1: of Hot water extract of Agaricus blazei Murrill specifically inhibits growth and induces apoptosis in human pancreatic cancer cells

Table S1. Genes with a statistically significant (greater than four-fold) change in expression in human pancreatic cancer cells treated with hot water extract of Agaricus blazei Murrill relative to the gene expression induced by mock treatment. (XLS 28046 kb

Additional file 4: of Hot water extract of Agaricus blazei Murrill specifically inhibits growth and induces apoptosis in human pancreatic cancer cells

Table S4–1. Overrepresentation test using REACTOME. Table S4–2. Genes overrepresented by REACTOME analysis. (XLS 31 kb

Additional file 5: of Hot water extract of Agaricus blazei Murrill specifically inhibits growth and induces apoptosis in human pancreatic cancer cells

Table S5–1. GO Biological Process annotations for genes altered only in MIA PaCa-2 by AbE treatment. Table S5–2. GO Biological Process annotations for genes altered only in PCI-35 by AbE treatment. Table S5–3. GO Biological Process annotations for genes altered only in PK-8 by AbE treatment. (XLS 996 kb

Additional file 2: of Hot water extract of Agaricus blazei Murrill specifically inhibits growth and induces apoptosis in human pancreatic cancer cells

Tables S2. PANTHER Overrepresentation Test based on Gene Ontology (GO) data. (XLS 40 kb

Rat primers used for RT-PCR analysis.

<p><i>Bcl2</i>, B-cell lymphoma 2;</p><p><i>Bcl-xl</i>, B-cell lymphoma-extra large;</p><p><i>MCP1</i>, monocyte chemoattractant protein 1;</p><p><i>HIF1a</i>, hypoxia induced factor 1a.</p><p>Rat primers used for RT-PCR analysis.</p

Effects of VPA on proliferation and inflammation.

<p>(A) PCNA, (B) SMA, and (C) ED1 immunohistochemical staining quantitative analysis of (D) the index of proliferation (the number of PCNA-positive cells per pulmonary vessel) and (E) the index of inflammation (the number of ED1-positive cells per pulmonary vessel) were used to show the regulation of proliferation and inflammation by VPA in the peripheral pulmonary vessels. Scale bar, 50 μm. * p < 0.05; *** p < 0.001.</p

Therapeutic effects of valproic acid (VPA) on severe PH.

<p>(A) Schematic of therapeutic protocols used in the control, prevention, and reversal studies. The comparison of (B) body weight change ratio, (C) systemic blood pressure (SBP), (D) right ventricular systolic pressure (RVSP), (E) Fulton index, (F) the ratio of right ventricular weight to body weight (RV/BW), (G) medial wall thickness, (H) muscularization ratio and (I) the vascular occlusion score (VOS) of small PAs between the Vehicle-treated group and the VPA-treated group (n = 6 per group). * p < 0.05; ** p < 0.01; *** p < 0.001.</p

Effects of VPA on gene transcription.

<p>(A) <i>HIF1a</i>, (B) <i>P21</i>, (C) <i>MCP1</i>, (D) <i>Casp3</i>, (E) <i>Bcl2</i>, (F) <i>Bcl-xl</i> mRNA levels were assessed using real-time reverse transcription-polymerase chain reaction (RT-PCR) and are shown as fold change relative to the expression level of the control group. * p < 0.05; ** p < 0.01; *** p < 0.001. <i>Bcl2</i>, B-cell lymphoma 2; <i>Bcl-xl</i>, B-cell lymphoma-extra large; <i>MCP1</i>, monocyte chemoattractant protein 1; <i>HIF1a</i>, hypoxia induced factor 1a.</p

Morphological and immunohistochemical analysis of pulmonary arteries (PAs) in severe PH rats.

<p>MCT/CH resulted in vascular occlusive neointimal lesions. (A) Victoria blue staining and α-smooth muscle actin (SMA) immunohistochemical staining were used to delineate the elastic membrane and media of PAs in control and MCT/CH rats at 3 weeks (MCT/CH 3w), 4 weeks (MCT/CH 4w), and 5 weeks (MCT/CH 5w). (B) Immunohistochemical staining for (a) fetal liver kinase 1 (FLK1), (b) ED1, (c) proliferating cell nuclear antigen (PCNA) and (d) cleaved caspase-3 in lung tissue sections (arrows) from rats with severe PH (sections from control rats are not shown). Scale bar, 50 μm. (C) Occlusive neointimal lesions occurred distal to the branch points of small muscularized PAs, and showed positive ED1 and PCNA staining. Scale bar, 50 μm.</p