Relationship between rCsCP concentrations and OD values.

<p>Bovine serum slbumin (BSA) was used as a negative control. OD values of rCsCp are expressed as mean OD values measured with IgY-IMB-ELISA from two independent combined experiments.</p

Detection results in sera of human clonorchiasis with different egg per gram faces (EPG) by IgY-IMB-ELISA.

<p>Each sample had duplicate tubes and the experiment was repeated twice.</p><p>Detection results in sera of human clonorchiasis with different egg per gram faces (EPG) by IgY-IMB-ELISA.</p

Comparison of OD values obtained in IgY-IMB-ELISA for detection of serum circulating antigens from clonorchiasis patients with different infection intensity (egg per gram of feces, EPG).

<p>Comparison of OD values obtained in IgY-IMB-ELISA for detection of serum circulating antigens from clonorchiasis patients with different infection intensity (egg per gram of feces, EPG).</p

Analysis of IgY by 12% SDS-PAGE in non-reducing condition.

<p>4 µg of protein was loaded for each lane. M: Protein molecular weight standards marker; 1: IgY from immunized egg yolk; 2: IgY from non-immunized egg yolk.</p

Analysis of IgY by Western blot.

<p>M: Protein molecular weight standards marker; 1: rCsCP probed with purified IgY from immunized egg yolk; 2: rCsCP probed with purified IgY from non-immunized egg yolk.</p

High-Performance Colorimetric Detection of Hg²⁺ Based on Triangular Silver Nanoprisms

Mercury ion (Hg²⁺) arising from a variety of natural sources and industrial wastes has been widely recognized as one of the most hazardous pollutants. It is very important to develop highly selective and sensitive probe for rapid detection of Hg²⁺ in aquatic ecosystems. Here we propose a new strategy for high-performance colorimetric detection of Hg²⁺, i.e., anti-etching of silver nanoprisms (AgNPRs). In the absence of Hg²⁺, the AgNPRs can be etched by I^– inducing an obvious color change from blue to red. However, in the presence of Hg²⁺, the formation of Ag–Hg nanoalloy can protect the AgNPRs from I^– etching and the color remains blue. This mechanism is verified by UV–vis, TEM, DLS, and EDS. Our AgNPRs-based colorimetric probe exhibits excellent selectivity for Hg²⁺. The limit of detection (LOD) of Hg²⁺ is 30 nM by the naked eye and 3 nM by UV–vis spectroscopy, which is lower than the mercury toxic level defined by the U.S. Environmental Protection Agency (10 nM). A good linear relationship (<i>R</i>² = 0.993) between the wavelength shift and Hg²⁺ concentrations indicates that our probe can be used for the quantitative assay of Hg²⁺. The results of Hg²⁺ detection in real environmental samples indicate the feasibility and sensitivity of our probe for application in complicated environmental samples