Dual Regulation of Sulfonated Lignin to Prevent and Treat Type 2 Diabetes Mellitus

With the rapid increase of diabetes cases in the world, there is an increasing demand for slowing down and managing diabetes and its effects. It is considered that a viable prophylactic treatment for type 2 diabetes mellitus (T2DM) is to reduce carbohydrate digestibility by controlling the activities of α-amylase and α-glucosidase to control postprandial hyperglycemia and promote the growth of intestinal beneficial bacteria. In this work, the effects of sulfonated lignin with different sulfonation degrees (0.8 mmol/g, SL1; 2.9 mmol/g, SL2) on the inhibition of α-amylase and α-glucosidase and the proliferation of intestinal beneficial bacteria in vitro were investigated. The results showed that both SL1 and SL2 can inhibit the activity of α-amylase and α-glucosidase. The inhibition capacity (IC50, 32.35 μg/mL) of SL2 with a low concentration (0–0.5 mg/mL) to α-amylase was close to that of acarbose to α-amylase (IC50, 27.33 μg/mL). Compared with the control groups, the bacterial cell concentrations of Bifidobacteria adolescentis and Lactobacillus acidophilus cultured with SL1 and SL2 increased in varying degrees (8–36%), and the produced short-chain fatty acids were about 1.2 times higher. This work demonstrates the prospect of sulfonated lignin as a prebiotic for the prevention and treatment of T2DM, which provides new insights for opening up a brand new field of lignin

Comparison of the Structural Characteristics of Cellulolytic Enzyme Lignin Preparations Isolated from Wheat Straw Stem and Leaf

Lignin structure has been considered to be an important factor that significantly influences the biorefinery processes. In this work, the effect of ball milling on the structural components and extractable lignin in enzymatic residues was evaluated, and the structural characteristics of the cellulolytic enzyme lignin preparations isolated from wheat straw stem (SCEL) and leaf (LCEL) were comparatively investigated by a combination of nitrobenzene oxidation (NBO), ozonation, infrared spectroscopy, and 1H–13C heteronuclear single quantum coherence nuclear magnetic resonance (2D HSQC NMR). The results showed that 4 h ball-milled samples were good enough for structural analysis with high lignin yield. Both CELs are typical p-hydroxyphenyl-guaiacyl-syringyl lignins which are associated with p-coumarates and ferulates. However, the structure of lignin in wheat straw stem is rather different from that in leaf. Compared to stem lignin, leaf lignin has lower product yields of NBO and ozonation, lower erythro/threo ratio, and higher condensation degree. The analysis of 2D HSQC NMR indicated that the S/G ratio of SCEL was 0.8, which is about twice as much as that of LCEL. The flavone tricin is incorporated into both stem and leaf lignins. The content of tricin in LCEL is higher than that in SCEL

Toward Strong and Tough Wood-Based Hydrogels for Sensors

The purpose of this research is to develop strong and tough wood-based hydrogels, which are reinforced by an aligned cellulosic wood skeleton. The hypothesis is that improved interfacial interaction between the wood cell wall and a polymer is of great importance for improving the mechanical performance. To this end, a facile and green approach, called ultraviolet (UV) grafting, was performed on the polyacrylamide (PAM)-infiltrated wood skeleton without using initiators. An important finding was that PAM-grafted cellulose nanofiber (CNF) architectures formed in the obtained hydrogels under UV irradiation, where CNFs themselves serve as both initiators and cross-linkers. Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency. The resulting alkali-treated Wood-g-PAM hydrogels exhibited significantly higher tensile properties than those of the Wood/PAM hydrogel and were further assembled into conductive devices for sensor applications. We believe that this UV grafting strategy may facilitate the development of strong wood-based composites with interesting features

Toward Strong and Tough Wood-Based Hydrogels for Sensors

The purpose of this research is to develop strong and tough wood-based hydrogels, which are reinforced by an aligned cellulosic wood skeleton. The hypothesis is that improved interfacial interaction between the wood cell wall and a polymer is of great importance for improving the mechanical performance. To this end, a facile and green approach, called ultraviolet (UV) grafting, was performed on the polyacrylamide (PAM)-infiltrated wood skeleton without using initiators. An important finding was that PAM-grafted cellulose nanofiber (CNF) architectures formed in the obtained hydrogels under UV irradiation, where CNFs themselves serve as both initiators and cross-linkers. Moreover, an alkali swelling treatment was utilized to improve the accessibility of the wood cell wall before UV irradiation and thus facilitate grafting efficiency. The resulting alkali-treated Wood-g-PAM hydrogels exhibited significantly higher tensile properties than those of the Wood/PAM hydrogel and were further assembled into conductive devices for sensor applications. We believe that this UV grafting strategy may facilitate the development of strong wood-based composites with interesting features

Mechanisms of Strain-Induced Interfacial Strengthening of Wet-Spun Filaments

We investigate the mechanism of binding of dopamine-conjugated carboxymethyl cellulose (DA-CMC) with carbon nanotubes (CNTs) and the strain-induced interfacial strengthening that takes place upon wet drawing and stretching filaments produced by wet-spinning. The filaments are known for their tensile strength (as high as 972 MPa and Young modulus of 84 GPa) and electrical conductivity (241 S cm–1). The role of axial orientation in the development of interfacial interactions and structural changes, enabling shear load bearing, is studied by molecular dynamics simulation, which further reveals the elasto-plasticity of the system. We propose that the reversible torsion of vicinal molecules and DA-CMC wrapping around CNTs are the main contributions to the interfacial strengthening of the filaments. Such effects play important roles in impacting the properties of filaments, including those related to electrothermal heating and sensing. Our findings contribute to a better understanding of high aspect nanoparticle assembly and alignment to achieve high-performance filaments

Uniform Micronano Network Affords All-Chitin Films with Water Resistance, Biodegradability, and High Strength

Xuan paper can be moistened without breaking, thus possessing good water resistance. These remarkable properties come from its uniform three-dimensional network structure constructed by micro- and nanofibers. Inspired by the multiscale structural design of Xuan paper, herein, we report a facile method to develop high-performance all-chitin films from crab shell wastes by introducing chitin nanofibers (ChNFs) into chitin microfiber (ChMF) networks. The resultant chitin micronanofiber (ChMNF) films integrated a high tensile strength (∼227.0 MPa), a low thermal expansion coefficient (∼10.3 ppm/K), high light transmittance (∼89.1%), and biodegradability. Moreover, this micronano structure endowed the chitin films with good water resistance and a high wet strength of ∼48.7 MPa which surpassed that of commercial paper (∼1.2 MPa) and some petroleum-based plastics (e.g., polyethylene, ∼24.8 MPa). One interesting finding was that after recycling treatments, the obtained recycled chitin films still showed a high strength of ∼126.2 MPa, similar to the chitin films with nanofiber structures (∼128.4 MPa). Moreover, the developed all-chitin films could be biodegraded in a natural environment in 3 months. Due to the combination of degradability, high strength, water resistance, transparency, and thermal stability, the flexible ChMNF films could be employed as environmentally friendly film materials applied in smart packaging and flexible electronics

Mechanisms of Strain-Induced Interfacial Strengthening of Wet-Spun Filaments

We investigate the mechanism of binding of dopamine-conjugated carboxymethyl cellulose (DA-CMC) with carbon nanotubes (CNTs) and the strain-induced interfacial strengthening that takes place upon wet drawing and stretching filaments produced by wet-spinning. The filaments are known for their tensile strength (as high as 972 MPa and Young modulus of 84 GPa) and electrical conductivity (241 S cm–1). The role of axial orientation in the development of interfacial interactions and structural changes, enabling shear load bearing, is studied by molecular dynamics simulation, which further reveals the elasto-plasticity of the system. We propose that the reversible torsion of vicinal molecules and DA-CMC wrapping around CNTs are the main contributions to the interfacial strengthening of the filaments. Such effects play important roles in impacting the properties of filaments, including those related to electrothermal heating and sensing. Our findings contribute to a better understanding of high aspect nanoparticle assembly and alignment to achieve high-performance filaments

Antitumor Effects of Carrier-Free Functionalized Lignin Materials on Human Hepatocellular Carcinoma (HepG2) Cells

Lignin, as an abundant aromatic biopolymer in plants, has great potential for medical applications due to its active sites, antioxidant activity, low biotoxicity, and good biocompatibility. In this work, a simple and ecofriendly approach for lignin fractionation and modification was developed to improve the antitumor activity of lignin. The lignin fraction KL-3 obtained by the lignin gradient acid precipitation at pH = 9–13 showed good cytotoxicity. Furthermore, the cell-feeding lignin after additional structural modifications such as demethylation (DKL-3), sulfonation (SL-3), and demethylsulfonation (DSKL-3) could exhibit higher glutathione responsiveness in the tumor microenvironment, resulting in reactive oxygen species accumulation and mitochondrial damage and eventually leading to apoptosis in HepG2 cells with minimal damage to normal cells. The IC50 values for KL-3, SL-3, and DSKL-3 were 0.71, 0.57, and 0.41 mg/mL, respectively, which were superior to those of other biomass extractives or unmodified lignin. Importantly, in vivo experiments conducted in nude mouse models demonstrated good biosafety and effective tumor destruction. This work provides a promising example of constructing carrier-free functionalized lignin antitumor materials with different structures for inhibiting the growth of human hepatocellular carcinoma (HepG2) cells, which is expected to improve cancer therapy outcomes