Schematic illustration of experimental design.

<p>Schematic illustration of experimental design.</p

Lateral radiographic images of the ankle at different times.

<p>White arrowheads indicate osteolytic changes inside the talus, and white triangles indicate titanium rods.</p

Characteristics of the porous titanium alloy.

<p>Characteristics of the porous titanium alloy.</p

Quantitative analysis of the parameters for reconstructed trabeculae, as calculated by micro-CT.

<p>Quantitative analysis of the parameters for reconstructed trabeculae, as calculated by micro-CT.</p

The 3-D reconstruction of talar specimens performed by micro-CT.

<p>Quantitative study showed that the trabecular reconstruction of the IM group was prior to that of the DC group at each time point.</p

Sample of a porous titanium alloy rod and gross specimens at 3 months after treatment.

<p>Blue arrowheads indicate treatment sites.</p

Histological examination of the DC and IM groups.

<p>The sections showed that the trabecular reconstruction was remarkable mainly in the IM group at 3 months after treatment, and the combination of the trabeculae and titanium alloy was very tight (Van Gieson stain, original magnification: left ×16; right ×100).</p

Section design for histology.

<p>Sections A, B, and C were prepared for staining.</p

Trinotate annotation

The taimen transcirptome was annotated using Trinotate(https://trinotate.github.io/) according to the guidance. NR, Uniprot-Sprot and Pfam databases were used

Percentages of reconstructed trabeculae in the ROIs of the two groups by histological study (<i>n</i> = 6, x ± s).

<p>Percentages of reconstructed trabeculae in the ROIs of the two groups by histological study (<i>n</i> = 6, x ± s).</p