Scatter plot analysis of two sample pairs (sc VS st, rc VS rt) from barnyardgrass.

<p>sc: untreated susceptible biotype; st: herbicide-treated susceptible biotype; rc: untreated resistant biotype; rt: herbicide-treated resistant biotype. Log2 transformed read counts of the mapped contigs and singletons were used for comparison.</p

Functional annotation of assembled sequences based on gene ontology (GO) terms.

<p>GO analysis was performed at level 2 for the three main categories (biological process, cellular component and molecular function).</p

Sequence length distribution of assembled contigs (A) and singletons (B) from the barnyardgrass 454 transcriptome.

<p>Sequence length distribution of assembled contigs (A) and singletons (B) from the barnyardgrass 454 transcriptome.</p

Distribution of the raw read sequence lengths from the barnyardgrass 454 transcriptome.

<p>Distribution of the raw read sequence lengths from the barnyardgrass 454 transcriptome.</p

Sequence ID, annotation, hit size and digital expression profile of tubulin genes in barnyardgrass (<i>E. crus-galli</i>).

<p>SC: untreated susceptible biotype; ST: herbicide-treated susceptible biotype; RC: untreated resistant biotype; RT: herbicide-treated resistant biotype.</p

Summary of barnyardgrass 454 transcriptome sequencing, assembly and annotation.

<p>Summary of barnyardgrass 454 transcriptome sequencing, assembly and annotation.</p

A list of <i>Echinochloa</i> genes with homology to barnyardgrass sequences.

<p>A list of <i>Echinochloa</i> genes with homology to barnyardgrass sequences.</p

Sequence ID, annotation, hit size and digital expression profile of acetolactate synthase.

<p>Sequence ID, annotation, hit size and digital expression profile of acetolactate synthase.</p

The growth of resistant (R) and susceptible (S) barnyardgrass biotypes at various concentrations of the herbicides quinclorac (A), bispyribac-sodium (B) and penoxsulam (C).

<p>The dose of each herbicide is in µmol.</p

The top 15 genes with highest expression levels in the transcriptome data.

<p>The top 15 genes with highest expression levels in the transcriptome data.</p