Landscape diversity index (Simpson’s <i>D</i>) and the proportion of other landscape variables at four spatial scales across all 33 sites.

<p>Landscape diversity index (Simpson’s <i>D</i>) and the proportion of other landscape variables at four spatial scales across all 33 sites.</p

Percent of variation accounted for by Partial Least Squares (PLS) regression analysis of the parasitism rate.

<p>Percent of variation accounted for by Partial Least Squares (PLS) regression analysis of the parasitism rate.</p

Model output for predicted (landscape) and response (parasitism rate) variables used in the Partial Least Squares (PLS) regression analysis.

<p>Model output for predicted (landscape) and response (parasitism rate) variables used in the Partial Least Squares (PLS) regression analysis.</p

Correlation loadings for the plot of the response variable (parasitism rate, Y) and predictor variables across four spatial scales based on PLS analysis.

<p>Radius: (a) 0.5 km; (b) 1.0 km; (c) 1.5 km; (d) 2.0 km. The four dotted line circles (25%, 50%, 75% and 100%) indicate the percentage range of variation explained by the first two factors. The distance of marked points from the origin and from the horizontal and vertical axes jointly show the amount of variation in each variable accounted for by the first two factors.</p

Summary statistics of RNA-seq library sequencing and mapping.

<p>Summary statistics of RNA-seq library sequencing and mapping.</p

qPCR validation of 8 genes from three CLR developmental stages. (a) Comparison of expression levels measured by RNAseq and qPCR for the selected 8 transcripts in three libraries (5L, Prepupae and Pupae; R² = 0.3569). (b) Comparison of expression levels of eight genes recorded by RNAseq and qPCR. Each panel shows gene expression on the left y-axis, determined by RPKM, and relative gene expression determined by qPCR on the right y-axis.

<p>qPCR validation of 8 genes from three CLR developmental stages. (a) Comparison of expression levels measured by RNAseq and qPCR for the selected 8 transcripts in three libraries (5L, Prepupae and Pupae; R² = 0.3569). (b) Comparison of expression levels of eight genes recorded by RNAseq and qPCR. Each panel shows gene expression on the left y-axis, determined by RPKM, and relative gene expression determined by qPCR on the right y-axis.</p

Expression pattern of a fibroin light chain gene at the indicated CLR developmental stages. Insect life stages are indicated on the vertical axis and relative gene expression (as fold-change) on the horizontal axis. Histogram bars annotated with the same letter are not significantly different.

<p>Expression pattern of a fibroin light chain gene at the indicated CLR developmental stages. Insect life stages are indicated on the vertical axis and relative gene expression (as fold-change) on the horizontal axis. Histogram bars annotated with the same letter are not significantly different.</p

Primers used for qRT-PCR validation, all with Tm = 55°C. Gene IDs are in parenthesis.

<p>Primers used for qRT-PCR validation, all with Tm = 55°C. Gene IDs are in parenthesis.</p

Twenty of highly differentially expressed annotated genes in the 5th instar larvae/ prepupae and prepupae/pupae libraries based on expressed tag frequency. “-”indicates down regulation.

<p>Twenty of highly differentially expressed annotated genes in the 5th instar larvae/ prepupae and prepupae/pupae libraries based on expressed tag frequency. “-”indicates down regulation.</p

A pie chart showing proportions (with numbers in parentheses) of transcripts annotated by NR, Swiss-prot, NT and KEGG databases.

<p>A pie chart showing proportions (with numbers in parentheses) of transcripts annotated by NR, Swiss-prot, NT and KEGG databases.</p