11 research outputs found


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    Characteristics of organic substance of the sediments of the West Lake are discussed in detail, including humic substance components and IR characteristics. The results showed that humus in the sediments of the West Lake is a typical lake sediment humus, and its humification level is high. Analysis on components of humus in the West Lake showed humin (59-71%) > humic acid (11-19%) > fulvic acid (8-15%) > lipid (3-12%). The Qianjiang River diversion project along with the planting of a lotus have both changed the organic composition of the sediment and will likely have an impact on the carbon cycling and eutrophication status in the West Lake.Article信州大学理学部附属諏訪臨湖実験所報告 11: 79-82(1999)departmental bulletin pape


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    The paper investigates the release of phosphorus release of West Lake sediments at various alkaline levels and disturbances during a laboratory simulation. The distribution of phosphate forms in the sediments and the content of phosphorus in interstitial water were also determined.Article信州大学理学部附属諏訪臨湖実験所報告 11: 83-86(1999)departmental bulletin pape

    Development of Sandwich and Competitive ELISA Formats to Determine Soybean Allergen: Evaluation of Their Performance to Detect Soy in Processed Food

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    Taking soybean mixed allergens as the target, a sandwich-enzyme linked immunosorbent assay and an indirect competitive enzyme-linked immunosorbent assay for the rapid and convenient detection of soybean allergens were established. The two methods were compared through the recovery experiment of actual processed samples, the recovery experiment of spiked food and the detection of real food samples, and their respective application scopes were determined. Sandwich ELISA method, the standard concentration was in the range of 0.0078~30 μg/mL, showing a good linear relationship, the curve equation was y=0.2333x+0.0692 with coefficient of determination R2=0.995. The detection range of the competitive ELISA method was 10~100000 ng/mL, and the lowest detection limit was 10 ng/mL. The recovery rate of orange juice after sandwich ELISA was higher than that after competitive ELISA, reaching more than 100%. For chocolate, beef sauce, bread and cake with complex ingredients and processing methods, the recovery rate after competitive ELISA was higher than that after double-antibody sandwich ELISA. In the detection of soybean allergens in fermented foods, the concentration detected by competitive ELISA was higher than that detected by sandwich ELISA, while in the detection of simple ingredients such as sesame paste and soy milk, the concentration detected by sandwich ELISA was slightly higher than that detected by competitive ELISA. In general, the competitive ELISA method was more suitable for the food with complex food matrix and deeply processed, while the sandwich ELISA method was more suitable for the food with simple food ingredients and lightly processed food, both of the two methods can achieve more accurate detection in their respective application scope

    Conformation and mechanical property of rpoS mRNA inhibitory stem studied by optical tweezers and X-ray scattering.

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    3' downstream inhibitory stem plays a crucial role in locking rpoS mRNA 5' untranslated region in a self-inhibitory state. Here, we used optical tweezers to study the unfolding/refolding of rpoS inhibitory stem in the absence and presence of Mg2+. We found adding Mg2+ decreased the free energy of the RNA junction without re-arranging its secondary structure, through confirming that this RNA formed a canonical RNA three-way junction. We suspected increased free energy might change the relative orientation of different stems of rpoS and confirmed this by small angle X-ray scattering. Such changed conformation may improve Hfq-bridged annealing between sRNA and rpoS RNA inhibitory stem. We established a convenient route to analyze the changes of RNA conformation and folding dynamics by combining optical tweezers with X-ray scattering methods. This route can be easily applied in the studies of other RNA structure and ligand-RNA