PCA and RDA analysis data sheet

The data were collected in the field. Excel was used to create the data. Gram+-gram positive bacteria, Gram--gram negative bacteria, AMF- arbuscular mycorrhizal fungi, B/F-bacteria to fungi ratio, SOC- soil organic carbon, TN- soil total nitrogen, C/N-soil organic carbon to total nitrogen ratio. Treatment: C- control,DU-dung and urine return; M-mowing; T-trampling; DU+M-mowing combined with the addition of dung and urine; M+T-mowing combined with trampling; DU+T-trampling combined with the addition of dung and urine; DU+M+T-mowing combined with trampling and the addition of dung and urin

Soil parameters

The data were collected in the field. Excel was used to create the data. SE-standard error, MBC-soil microbial biomass carbon, MBN- soil microbial biomass nitrogen. Treatment: C- control,DU-dung and urine return; M-mowing; T-trampling; DU+M-mowing combined with the addition of dung and urine; M+T-mowing combined with trampling; DU+T-trampling combined with the addition of dung and urine; DU+M+T-mowing combined with trampling and the addition of dung and urin

Soil microbes' parameters

The data were collected in the field. Excel was used to create the data. SE-standard error, Gram+-gram positive bacteria, Gram--gram negative bacteria, AMF- arbuscular mycorrhizal fungi, B/F-bacteria to fungi ratio. Treatment: C- control,DU-dung and urine return; M-mowing; T-trampling; DU+M-mowing combined with the addition of dung and urine; M+T-mowing combined with trampling; DU+T-trampling combined with the addition of dung and urine; DU+M+T-mowing combined with trampling and the addition of dung and urin

Molybdate in Rhizobial Seed-Coat Formulations Improves the Production and Nodulation of Alfalfa

<div><p>Rhizobia-legume symbiosis is the most well researched biological nitrogen fixation system. Coating legume seeds with rhizobia is now a recognized practical measure for improving the production of legume corp. However, the efficacy of some commercial rhizobia inoculants cannot be guaranteed in China due to the low rate of live rhizobia in these products. A greenhouse experiment was conducted to assess the effects of different rhizobial inoculant formulations on alfalfa productivity and nitrogen fixation. Two rhizobia strains, (ACCC17631 and ACCC17676), that are effective partners with alfalfa variety Zhongmu No. 1 were assessed with different concentrations of ammonium molybdate in seed-coat formulations with two different coating adhesives. Our study showed that the growth, nodulation, and nitrogen fixation ability of the plants inoculated with the ACCC17631 rhizobial strain were greatest when the ammonium molybdate application was0.2% of the formulation. An ammonium molybdate concentration of 0.1% was most beneficial to the growth of the plants inoculated with the ACCC17676 rhizobial strain. The sodium carboxymethyl cellulose and sodium alginate, used as coating adhesives, did not have a significant effect on alfalfa biomass and nitrogen fixation. However, the addition of skimmed milk to the adhesive improved nitrogenase activity. These results demonstrate that a new rhizobial seed-coat formulation benefitted alfalfa nodulation and yield.</p></div

Analysis of variance for the effects of alkalinity stress (A), rhizome connection (C) and data (D) on the tiller number and height of subsequent ramets.

<p>Note: <i>F</i>-values are shown for each variable followed by their respective significance levels.</p><p>*<i>P</i> < 0.05;</p><p>**<i>P</i> < 0.01;</p><p>***<i>P</i> <0.001;</p><p>^ns, <i>P</i> > 0.05.</p><p>Analysis of variance for the effects of alkalinity stress (A), rhizome connection (C) and data (D) on the tiller number and height of subsequent ramets.</p

Effects of alkalinity stress, rhizome connection and data on the tiller number and height of subsequent ramets.

<p>Solid lines with 0C, 60C, 180C and 300C represented 0/0, 0/60, 0/180 and 0/300 mmol L^-1 alkalinity levels in the rhizome-connected treatments, respectively; Dash lines with 0S, 60S, 180S and 300S represented 0/0, 0/60, 0/180 and 0/300 mmol L^-1 alkalinity levels in the rhizome-severed treatments, respectively.</p

Analysis of variance for the effects of alkalinity stress (A) and rhizome connection (C) on the biomass of original and subsequent ramets and cost-benefit analysis.

<p>Note: <i>F</i>-values are shown for each variable followed by their respective significance levels.</p><p>*<i>P</i> < 0.05;</p><p>**<i>P</i> < 0.01;</p><p>***<i>P</i> <0.001;</p><p>^ns, <i>P</i> > 0.05.</p><p>Analysis of variance for the effects of alkalinity stress (A) and rhizome connection (C) on the biomass of original and subsequent ramets and cost-benefit analysis.</p

Effects of alkalinity stress and rhizome connection on a) root weight, b) rhizome length, c) bud number, d) photosynthetic rate and e) water potential of subsequent ramets.

<p>Bar groups with different capital letters indicate significant differences (<i>P</i> < 0.05) between the four levels of alkalinity in the rhizome-connected treatment. Bar groups with different lowercase letters indicate significant differences (<i>P</i> < 0.05) between the four levels of alkalinity in the rhizome-severed treatment. Asterisks indicate significant differences (<i>P</i> < 0.05) between rhizome-connected and rhizome-severed treatments.</p

Effects of alkalinity stress and rhizome connection on a) the biomass of daughter and b) mother ramets, c) the total biomass of clonal organs and d) cost-benefits of clonal integration.

<p>Bar groups with different capital letters indicate significant differences (<i>P</i> < 0.05) between the four levels of alkalinity in the rhizome-connected treatment. Bar groups with different lowercase letters indicate significant differences (<i>P</i> < 0.05) between the four levels of alkalinity in the rhizome-severed treatment. Asterisks indicate significant differences (<i>P</i> < 0.05) between rhizome-connected and rhizome-severed treatments.</p

Analysis of variance for the effects of different Mo concentrations, adhesive agents and their interactions on plant height, aboveground biomass, the number of nodules, nodule weight and nitrogenase activity in alfalfa.

<p>Analysis of variance for the effects of different Mo concentrations, adhesive agents and their interactions on plant height, aboveground biomass, the number of nodules, nodule weight and nitrogenase activity in alfalfa.</p