Veterinarians in the UK on the use of non-steroidal anti-inflammatory drugs (NSAIDs) for post-disbudding analgesia of calves

<p>Top 20 down-regulated genes after DAPT treatment in P0 Lfng-GFP⁺ cells.</p

Distribution of correlations between microarray and sequencing profiles for miRNA and gene expression.

<p>(A) Histogram of correlation coefficients for individual miRNAs measured by miRNA-Seq and miRNA array. (B) Histogram of correlation coefficients for mRNAs profiled by Illumina HiSeq and mRNA array. (C) The empirical cumulative distribution function (ECDF) of the correlation between array and sequencing for miRNA (black), filtered miRNA (color) and mRNA (gray) measurements. Nearly, 72% of miRNAs demonstrate a correlation coefficient ≤0.5 whereas 22% of RNAs have a correlation coefficient ≤0.5. When filtered based on expression level, the percentage of miRNAs with correlation ≤0.5 saturated to 56%.</p

Scatter-plots of microRNA expression measured by microarray and miRNA-Seq.

<p>(A) miR-98, (B) miR-505 (C) miR-143 and (D) miR-141.</p

MicroRNAs associated with ovarian cancer survival.

<p>P-value plots of univariate Cox regression for microRNAs associated with ovarian cancer survival identified by microarray (A) or miRNA-Seq (B) data. P-value<0.01 (Solid line). False discovery rate (FDR)<0.1 (Dotted line). In both A&B, blue dots indicate miRNAs associated with survival by miRNA array, while red dots indicate miRNAs associated with survival by miR-Seq. Green stars are miRNAs associated with survival in both datasets. (C) percentage of overlapping miRNAs between the array and NGS seq platform at different cut-off threshold for Cox p-values, BH adjusted FDR, and Storey q-values.</p

Top 20 genes enriched in Lfng-GFP⁺ cells at P6.

Top 20 genes enriched in Lfng-GFP+ cells at P6.</p

Notch and Wnt pathway genes expressed in Lfng-GFP⁺ cells at P1 and P6.

<p>Notch and Wnt pathway genes expressed in Lfng-GFP⁺ cells at P1 and P6.</p

Isolation of neonatal supporting cells and analysis by RNA-seq.

<p>(A) Sections through postnatal day 1 and 6 <i>LFng-GFP</i> transgenic mice. At both ages, the GFP transgene is expressed in border cells, inner phalangeal cells, outer pillar cells and all three rows of Deiters’ cells, but not inner pillar cells (arrowheads). Sections are counterstained with antibodies to MYOSIN VI to show hair cells and SOX2, PROX1, GLAST or NGFR to show distinct types of supporting cells (magenta). Scale bar 20 μm. (B) Representative FACS profile for sorting of P1 <i>LFng-GFP</i> transgenic cochleas. GFP intensity is shown on the x-axis, with four fractions (F1-F4) identified on the sorting profile. (C) QPCR analysis of the different fractions for expression of a hair cell marker (<i>Myo6</i>) and a supporting cell marker (<i>Prox1</i>). Cells falling in F3, which contained the highest <i>Prox1</i> signal and lowest <i>Myo6</i> signal were used in subsequent experiments. (D) Identification of transcripts enriched in the GFP⁺ fractions at P1 (left) and P6 (center) using the intersection of differential gene expression between GFP⁺ and GFP^- cells analyzed with DESeq and Cufflinks. 586 consensus GFP⁺ transcripts were identified at P1 and 508 at P6. The right Venn diagram shows the intersection of enriched GFP+ transcripts differentially expressed between P1 and P6 analyzed with DESeq and Cufflinks. 79 transcripts were enriched in P1 GFP⁺ cells compared to P6 GFP⁺ cells, whereas 259 transcripts were enriched in P6 GFP⁺ cells compared to P1 GFP⁺ cells. <i>p</i> < 0.01, q < 0.01 and log2(fold change) > 2.</p

Top 20 genes enriched in Lfng-GFP⁺ cells at P1.

<p>Top 20 genes enriched in Lfng-GFP⁺ cells at P1.</p

Biological processes (BP) GO terms represented in the genes enriched in P6 Lfng-GFP⁺ cells.

<p>Biological processes (BP) GO terms represented in the genes enriched in P6 Lfng-GFP⁺ cells.</p

Supplementary Data from Confirmation of Gene Expression–Based Prediction of Survival in Non–Small Cell Lung Cancer

Supplementary Data from Confirmation of Gene Expression–Based Prediction of Survival in Non–Small Cell Lung Cance