Effect of 1-MCP on storage quality and the mechanism involved in ethylene signal transduction in a new early-maturing apple variety ‘Taihangzaohong’ fruits during cold storage

1-Methylcyclopropene (1-MCP) can reduce the rate of fruit softening and prolong storage time. In this study, the fruit of a new early-maturing apple variety, ‘Taihangzaohong’, was treated with air (control), 2 μL/L 1-MCP, 100 μL/L ethylene (C 2H4) or 2 μL/L 1-MCP +100 μL/L C2H4 for 24 hours and then stored at 4 °C for 70 days. The postharvest physiological indices and the expression of 13 genes related to ethylene biosynthesis and signal transduction were monitored every 10 days. The results showed that 1-MCP can delay the softening rate and maintain the fruit quality of this early-maturing apple variety by reducing ethylene production by reducing the expression of MdACO1, MdACO2, and MdACS1, as well as by preventing ethylene signal transduction by decreasing the expression of MdETR2 and MdERS1 and increasing the expression of MdCTR1. Understanding the significant changes in these genes and their functions may help us explore the mechanisms controlling apple fruit softening and its response to exogenous 1-MCP and ethylene stimuli, as well as inhibition at the receptor level during ripening and senescence

Tolerance of transgenic Arabidopsis thaliana overexpressing apple MdAGO4.1 gene to drought and salt stress

The regulatory role of apple MdAGO4.1 gene in plant drought and salt resistance is unclear. In this study, transgenic A. thaliana in which the apple MdAGO4.1 gene was over-expressed was used to analyze the regulatory effects of the MdAGO4.1 gene on plant drought and salt resistance, to verify the function of the apple MdAGO4.1 gene. The seed germination rate, seedling fresh weight and root length of transgenic Arabidopsis strains in MS medium containing different concentrations of NaCl and mannitol were better than those of the wild type. The transgenic A. thaliana seedlings were more resistant to drought than wild type under drought stress. The transgenic strains were less affected by salt stress than thewild type. Exposure to drought and salt stress reduced the relative elektrolyte leakage, malondialdehyde (MDA), superoxide anion (O2-), and hydrogen peroxide (H2O2) levels of the transgenic strain significantly compared with the levels in the wild type. The levels of proline, protective enzyme activities, and the expression of genes related to drought and salt stress resistance were significantly higher than those of the wild type. These results indicate that MdAGO4.1 overexpression improved drought and salt tolerance in transgenic Arabidopsis. This study can provide a theoretical basis for future research on stress tolerance mechanisms and breeding new varieties of fruit trees resistant to drought and salt

Investigation of the Protective Effects of Phlorizin on Diabetic Cardiomyopathy in db/db Mice by Quantitative Proteomics

Patients with diabetes often develop hypertension and atherosclerosis leading to cardiovascular disease. However, some diabetic patients develop heart failure without hypertension and coronary artery disease, a process termed diabetic cardiomyopathy. Phlorizin has been reported to be effective as an antioxidant in treating diabetes mellitus, but little is known about its cardioprotective effects on diabetic cardiomyopathy. In this study, we investigated the role of phlorizin in preventing diabetic cardiomyopathy in db/db mice. We found that phlorizin significantly decreased body weight gain and the levels of serum fasting blood glucose (FBG), triglycerides (TG), total cholesterol (TC), and advanced glycation end products (AGEs). Morphologic observations showed that normal myocardial structure was better preserved after phlorizin treatment. Using isobaric tag for relative and absolute quantitation (iTRAQ) proteomics, we identified differentially expressed proteins involved in cardiac lipid metabolism, mitochondrial function, and cardiomyopathy, suggesting that phlorizin may prevent the development of diabetic cardiomyopathy by regulating the expression of key proteins in these processes. We used ingenuity pathway analysis (IPA) to generate an interaction network to map the pathways containing these proteins. Our findings provide important information about the mechanism of diabetic cardiomyopathy and also suggest that phlorizin may be a novel therapeutic approach for the treatment of diabetic cardiomyopathy

Effects of Dwarfing Interstock Length on the Growth and Fruit of Apple Tree

There is no report on the effect of the length of Jizhen 2 interstock on the growth and fruit quality of Tianhong 2 apple trees, which are usually grown in Baoding, Hebei Province, China. We surveyed the tree size, branch types, fruit set, fruit quality and root parameters of 3–5-year-old ‘Tianhong 2/Jizhen 2/Malus × robusta Rehder’ apple trees, to study the effects of dwarfing interstock length on the growth of the tree’s aboveground parts and roots, as well as fruit yield and quality. The tree height and the stem girths of the interstock and scion decreased as interstock length increased, and the dwarfing degree of the apple trees gradually increased. Trees with an interstock length of 30 cm had the fewest long branches, the most short branches, and the greatest proportion of short branches. An interstock length of 30 cm provided the highest fruit-set rate, the highest yield per tree and per unit cross-sectional area, the highest single fruit weight, the highest soluble: acid ratio, the highest color brightness (L*), and better red skin coloration (higher a*) of the fruit skin. The root length density, root surface area density, and root volume density exhibited two growth peaks in a year, during the slow growth period when spring and autumn shoots are stopped. Root length density, root surface area density, and root volume density decreased with interstock length. Root death peaked during the growth peak period of the autumn shoots, and root length density of dead roots and root turnover frequencyincreased with the interstock length. A 30 cm length was the most suitable for the Tianhong 2 apple trees when the Jizhen 2 was used as the interstock

TLFW: A Three-Layer Framework in Wireless Rechargeable Sensor Network with a Mobile Base Station

Wireless sensor networks as the base support for the Internet of things have been a large number of popularity and application. Such as intelligent agriculture, we have to use the sensor network to obtain the growing environment data of crops and others. However, the difficulty of power supply of wireless nodes has seriously hindered the application and development of Internet of things. In order to solve this problem, people use low-power sleep scheduling and other energy-saving methods on the nodes. Although these methods can prolong the working time of nodes, they will eventually become invalid because of the exhaustion of energy. The use of solar energy, wind energy, and wireless signals in the environment to obtain energy is another way to solve the energy problem of nodes. However, these methods are affected by weather, environment, and other factors, and they are unstable. Thus, the discontinuity work of the node is caused. In recent years, the development of wireless power transfer (WPT) has brought another solution to this problem. In this paper, a three-layer framework is proposed for mobile station data collection in rechargeable wireless sensor networks to keep the node running forever, named TLFW which includes the sensor layer, cluster head layer, and mobile station layer. And the framework can minimize the total energy consumption of the system. The simulation results show that the scheme can reduce the energy consumption of the entire system, compared with a Mobile Station in a Rechargeable Sensor Network (MSiRSN)

Neutralizing antibody levels associated with injectable and aerosolized Ad5-nCoV boosters and BA.2 infection

Abstract Background Several COVID-19 vaccines are in widespread use in China. Few data exist on comparative immunogenicity of different COVID-19 vaccines given as booster doses. We aimed to assess neutralizing antibody levels raised by injectable and inhaled aerosolized recombinant adenovirus type 5 (Ad5)-vectored COVID-19 vaccine as a heterologous booster after an inactivated COVID-19 vaccine two-dose primary series. Methods Using an open-label prospective cohort design, we recruited 136 individuals who had received inactivated vaccine primary series followed by either injectable or inhaled Ad5-vectored vaccine and measured neutralizing antibody titers against ancestral SARS-CoV-2 virus and Omicron BA.1 and BA.5 variants. We also measured neutralizing antibody levels in convalescent sera from 39 patients who recovered from Omicron BA.2 infection. Results Six months after primary series vaccination, neutralizing immunity against ancestral SARS-CoV-2 was low and neutralizing immunity against Omicron (B.1.1.529) was lower. Boosting with Ad5-vectored vaccines induced a high immune response against ancestral SARS-CoV-2. Neutralizing responses against Omicron BA.5 were ≥ 80% lower than against ancestral SARS-CoV-2 in sera from prime-boost subjects and in convalescent sera from survivors of Omicron BA.2 infection. Inhaled aerosolized Ad5-vectored vaccine was associated with greater neutralizing titers than injectable Ad5-vectored vaccine against ancestral and Omicron SARS-CoV-2 variants. Conclusions These findings support the current strategy of heterologous boosting with injectable or inhaled Ad5-vectored SARS-CoV-2 vaccination of individuals primed with inactivated COVID-19 vaccine