Supplementary material - Supplemental material for Testing modified zeros for Poisson regression models

Supplemental material, Supplementary material for Testing modified zeros for Poisson regression models by Yi Tang and Wan Tang in Statistical Methods in Medical Research</p

Biosynthesis of a Highly Stable Coiled-Coil Protein Containing Hexafluoroleucine in an Engineered Bacterial Host

Biosynthesis of a Highly Stable Coiled-Coil Protein Containing Hexafluoroleucine in an Engineered Bacterial Hos

The meaning of acoustic indices in this study.

The recognition of bird species through the analysis of their vocalizations is a crucial aspect of wildlife conservation and biodiversity monitoring. In this study, the acoustic features of Certhia americana, Certhia brachydactyla, and Certhia familiaris were calculated including the Acoustic complexity index (ACI), Acoustic diversity index (ADI), Acoustic evenness index (AEI), Bioacoustic index (BI), Median of the amplitude envelop (MA), and Normalized Difference Soundscape Index (NDSI). Three machine learning models, Random Forest (RF), Support Vector Machine (SVM), and Extreme Gradient Boosting (XGBoost), were constructed. The results showed that the XGBoost model had the best performance among the three models, with the highest accuracy (0.8365) and the highest AUC (0.8871). This suggests that XGBoost is an effective tool for bird species recognition based on acoustic indices. The study provides a new approach to bird species recognition that utilizes sound data and acoustic characteristics.</div

Biosynthesis of Amino Acid Derived α‑Pyrones by an NRPS–NRPKS Hybrid Megasynthetase in Fungi

A nonribosomal peptide synthetase (NRPS)-nonreducing polyketide synthase (NRPKS) hybrid enzyme (AnATPKS) from Aspergillus niger was shown to produce amino acid derived α-pyrone natural products (pyrophen and campyrone B). Biochemical characterization of the NRPS module in vitro reveals that the adenylation domain is promiscuous toward a variety of substituted phenylalanine analogues. Using precursor feeding and heterologous expression of AnATPKS and an associated O-methyltransferase (AnOMT), we were able to access a library of substituted pyrophen analogues. Our study paves the way for future combinatorial biosynthesis of diverse α-pyrone natural products using NRPS–NRPKS hybrids

The violin plots of acoustic indices grouped by species.

The violin plots of acoustic indices grouped by species.</p

The performace of machine learning models (Mean±SD).

The performace of machine learning models (Mean±SD).</p

Inhibition of Glucose-6-Phosphate Translocase Activity by Anthraquinones

(A) The chemical structure of mumbaistatin. Mumbaistatin is an extremely potent inhibitor of glucose-6-phosphate translocase (IC50 = 5 nM). The core of the molecule is a reduced carboxylic acid containing anthraquinone, which is also observed in compounds such as DMAC, YT127, and YT127b. (B) Inhibition of glucose-6-phosphate translocase activity by novel anthraquinones. The inhibition assay is performed as described in the experimental section. The control contains the rat liver microsome and glucose-6-phosphate only. Chlorogenic acid (IC50 = 0.26 mM) was used as a reference. The G6Pase activity of the microsome in the presence of six anthraquinones (DMAC, 3, 4, 7, 8, and 9) was measured. For each compound, three different concentrations (50, 25, and 12.5 mM) were used to detect dose-dependent inhibition. No inhibition was observed for DMAC or 7. Dose-dependent inhibition was observed for compounds 3, 4, 8, and 9.</p

Data flow diagram for recognition of bird species with birdsong records.

Data flow diagram for recognition of bird species with birdsong records.</p