94 research outputs found
Nyelv, nyelvtörvény, nyelvtudomåny
<p>4a, Forest plot on the associations between DM and bile leakage after hepatectomy. 4b, Forest plot on the associations between DM and ascites after hepatectomy. 4c, Forest plot on the associations between DM and liver decompensation after hepatectomy. DM, diabetes mellitus. The boxes and lines indicate the relative ratios (RRs) and their confidence intervals (CIs) on a log scale for each study. The pooled RR is represented by a diamond. The size of the black squares indicates the relative weight of each estimate.</p
Turn-on Persistent Luminescence Probe Based on Graphitic Carbon Nitride for Imaging Detection of Biothiols in Biological Fluids
Herein, we present a novel strategy
based on a âturn-onâ
persistent luminescence imaging chemical system of graphitic carbon
nitride for detecting biothiols in biological fluids. Graphitic carbon
nitride (g-C<sub>3</sub>N<sub>4</sub>) as persistent luminescence
probe is fabricated via a new procedure based on pyrolysis of guanidine
hydrochloride under ambient atmospheric conditions. The prepared g-C<sub>3</sub>N<sub>4</sub> nanosheets give intensively long-persistent
luminescence that can avoid interference from biological media such
as tissue autofluorescence and scattering light. The original persistent
luminescence of g-C<sub>3</sub>N<sub>4</sub> turns off due to the
adsorption of silver ion (Ag<sup>+</sup>) onto g-C<sub>3</sub>N<sub>4</sub> materials with an electron transfer process. The presence
of biothiols induces the onset of persistent luminescence emission
by interrupting the quenching interaction, thereby turning on the
imaging probe. The approach exhibits high specificity and high sensitivity
to biothiols with low detection limit for cysteine (Cys), homocysteine
(Hcy), and glutathione (GSH) with 6.4, 8.1, and 9.6 nM, respectively.
It is also successfully applied for imaging detection of biothiols
in human urine, plasma, and cell lysates, demonstrating its great
value of practical application in biological systems
Clinical outcomes of patients with and without diabetes mellitus after hepatectomy: A systematic review and meta-analysis
<div><p>Background</p><p>Clinical data regarding the influence of diabetes mellitus (DM) on the outcomes of patients undergoing hepatectomy are conflicting. To determine the impact of DM on the clinical outcomes of patients undergoing hepatectomy, we systematically reviewed published studies and carried out a meta-analysis.</p><p>Methods</p><p>A systematic literature search of Pubmed, Sciencedirect, Web of Science, and Chinese Biomedical Database was conducted from their inception through February 2, 2016. The combined relative risk (RR) or hazard ratio (HR) with 95% confidence intervals (95% CI) was calculated.</p><p>Results</p><p>A total of 16 observational studies with 15710 subjects were eligible for meta-analysis. The pooled results showed that DM significantly increased the risk of overall postoperative complications (RR 1.34; 95% CI 1.19â1.51; P<0.001), DM-associated complications (RR 1.8; 95% CI 1.29â2.53; P<0.001), liver failure (RR 2.21; 95% CI 1.3â3.76; P = 0.028) and post-operative infections (RR 1.59; 95% CI 1.01â2.5; P = 0.045). In addition, DM was also found to be significantly associated with unfavorable overall survival and disease free survival after liver resection. The pooled HR was 1.63 (95% CI 1.33â1.99; P<0.001) for overall survival and 1.55 (95% CI 1.07â2.25; P = 0.019) for disease free survival.</p><p>Conclusion</p><p>DM is associated with poor outcomes in patients undergoing hepatectomy. DM should be taken into account cautiously in the management of patients undergoing hepatectomy. Further prospective studies are warranted to explore effective interventions to improve the poor outcomes of diabetic patients undergoing hepatectomy.</p></div
Multisignals Sensing Platform for Highly Sensitive, Accurate, and Rapid Detection of <i>p</i>âAminophenol Based on Adsorption and Oxidation Effects Induced by Defective NH<sub>2</sub>âAg-nMOFs
Labile toxic pollutants detection
remains a challenge due to the
problem that a single method is prone to producing false-negative/-positive
signals. The construction of a multisignal sensing platform with the
advantages of different strategies is an effective way to solve this
problem. Herein, a novel resonant light scattering (RLS), fluorescent
and rapid visual multisignals sensing strategy for p-aminophenol (p-AP) detection was designed based
on the adsorption and oxidation effects of defective amino-functionalized
Ag-based nano metalâorganic frameworks (NH2-Ag-nMOFs).
In this reaction process, NH2-Ag-nMOFs with incomplete
coordination oxidize H2O2 to produce singlet
oxygen (1O2) which rapidly oxidizes p-AP, leading to the reduction of Ag+ to Ag0, thereby disrupting the structure of NH2-Ag-nMOFs
and resulting in fluorescence quenching of NH2-Ag-nMOFs.
Synchronously, owing to Ag0 aggregation and p-AP oxidation, the color of the system changed from colorless to
purplish-red and pale brown within 20 s. The assay has realized the
rapid naked-eye detection of 5 ÎŒM p-AP rapidly.
Additionally, thanks to the intermolecular hydrogen bonding, NH2-Ag-nMOFs-p-AP aggregates formed, which enhanced
the RLS signal. With the RLS signal, the designed multisignals sensing
platform can analyze p-AP at a concentration as low
as 11 nM and yield a wider dynamic response range than any single
signal strategy reported before, which can quickly meet the measurement
requirement of different actual samples. Overall, the proposed strategy
without assembling various signal indicators presented an accurate,
rapid, cost-effective, and sensitive multisignals sensing platform
for p-AP analysis and has great prospects in labile
toxic pollutants monitoring
Protein Quantitation Using Ru-NHS Ester Tagging and Isotope Dilution High-Pressure Liquid ChromatographyâInductively Coupled Plasma Mass Spectrometry Determination
An accurate, simple, and sensitive method for the direct
determination of proteins by nonspecies specific isotope dilution
and external calibration high-performance liquid chromatographyâinductively
coupled plasma mass spectrometry (HPLCâICPMS) is described.
The labeling of myoglobin (17 kDa), transferrin (77 kDa), and thyroglobulin
(670 kDa) proteins was accomplished in a single-step reaction with
a commercially available bisÂ(2,2âČ-bipyridine)-4âČ-methyl-4-carboxybipyridine-ruthenium <i>N</i>-succinimidyl ester-bisÂ(hexafluorophosphate) (Ru-NHS ester).
Using excess amounts of Ru-NHS ester compared to the protein concentration
at optimized labeling conditions, constant ratios for Ru to proteins
were obtained. Bioconjugate solutions containing both labeled and
unlabeled proteins as well as excess Ru-NHS ester reagent were injected
onto a size exclusion HPLC column for separation and ICPMS detection
without any further treatment. A <sup>99</sup>Ru enriched spike was
used for nonspecies specific ID calibration. The accuracy of the method
was confirmed at various concentration levels. An average recovery
of 100% ± 3% (1 standard deviation (SD), <i>n</i> =
9) was obtained with a typical precision of better than 5% RSD at
100 ÎŒg mL<sup>â1</sup> for nonspecies specific ID. Detection
limits (3SD) of 1.6, 3.2, and 7.0 fmol estimated from three procedure
blanks were obtained for myoglobin, transferrin, and thyroglobulin,
respectively. These detection limits are suitable for the direct determination
of intact proteins at trace levels. For simplicity, external calibration
was also tested. Good linear correlation coefficients, 0.9901, 0.9921,
and 0.9980 for myoglobin, transferrin, and thyroglobulin, respectively,
were obtained. The measured concentrations of proteins in a solution
were in good agreement with their volumetrically prepared values.
To the best of our knowledge, this is the first application of nonspecies
specific ID for the accurate and direct determination of proteins
using a Ru-NHS ester labeling reagent
Stratified analysis of the association between diabetes mellitus and prognosis after hepatectomy.
<p>Stratified analysis of the association between diabetes mellitus and prognosis after hepatectomy.</p
Image1_Overexpression of Fgf18 in cranial neural crest cells recapitulates Pierre Robin sequence in mice.pdf
The pivotal role of FGF18 in the regulation of craniofacial and skeletal development has been well established. Previous studies have demonstrated that mice with deficiency in Fgf18 exhibit severe craniofacial dysplasia. Recent clinical reports have revealed that the duplication of chromosome 5q32-35.3, which encompasses the Fgf18 gene, can lead to cranial bone dysplasia and congenital craniosynostosis, implicating the consequence of possible overdosed FGF18 signaling. This study aimed to test the effects of augmented FGF18 signaling by specifically overexpressing the Fgf18 gene in cranial neural crest cells using the Wnt1-Cre;pMes-Fgf18 mouse model. The results showed that overexpression of Fgf18 leads to craniofacial abnormalities in mice similar to the Pierre Robin sequence in humans, including abnormal tongue morphology, micrognathia, and cleft palate. Further examination revealed that elevated levels of Fgf18 activated the Akt and Erk signaling pathways, leading to an increase in the proliferation level of tongue tendon cells and alterations in the contraction pattern of the genioglossus muscle. Additionally, we observed that excessive FGF18 signaling contributed to the reduction in the length of Meckelâs cartilage and disrupted the development of condylar cartilage, ultimately resulting in mandibular defects. These anomalies involve changes in several downstream signals, including Runx2, p21, Akt, Erk, p38, Wnt, and Ihh. This study highlights the crucial role of maintaining the balance of endogenous FGF18 signaling for proper craniofacial development and offers insights into potential formation mechanisms of the Pierre Robin sequence.</p
Forest plot on the associations between DM and survival after hepatectomy.
<p>6a, Forest plot on the associations between DM and overall survival after hepatectomy. 6b, Forest plot on the associations between DM and disease-free survival after hepatectomy. DM, diabetes mellitus. The boxes and lines indicate the hazard ratios (HRs) and their confidence intervals (CIs) on a log scale for each study. The pooled HR is represented by a diamond. The size of the black squares indicates the relative weight of each estimate.</p
Label-Free DNA Assay by Metal Stable Isotope Detection
The
interest in label-free bioassays is increasing rapidly because
of their simple procedure and direct information on the interaction
between the target molecule and the sensing unit. One of the major
obstacles in the application of label-free biosensors is the difficulty
to produce stable and reproducible optical, electric, electrochemical,
or magnetic properties for the sensitive detection of the target molecules.
In this work, we demonstrated a label-free DNA assay, by directly
measuring the intrinsic <sup>63</sup>Cu and <sup>65</sup>Cu stable
isotopes inside the double-strand DNA-templated Cu nanoparticles.
The experimental conditions, including detection of copper by elemental
mass spectrometry, the copper nanoparticles formation parameters,
the hybrid chain reaction parameters, and analytical performance,
were investigated in detail. The <sup>63</sup>Cu signal intensity
possesses a linear relation with the concentration of target DNA over
the range of 20â1000 pM with a detection limit of 4 pM (3Ï).
The detection limit of this method is among the most sensitive label-free
techniques and also comparable to the lanthanides and Au nanoparticles
labeled assays by elemental mass spectrometric detection. The proposed
label-free bioassay is simple and sensitive and eliminated the need
for optical, electric, electrochemical, or magnetic properties of
the sensing unit. To our best knowledge, this is the first report
of the label-free bioassay by metal stable isotope detection
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