Additional file 4 of WNT16 elevation induced cell senescence of osteoblasts in ankylosing spondylitis

Additional file 4: Supplementary Figure 4. WNT16 treatment inhibited ALP activity in AS-osteoprogenitors. AS-osteoprogenitors were plated in 96 wells plate and treated with 0, 25, or 50 ng/ml WNT16, and then analyzed as indicated by (A) WST assay for cell proliferation. (B) ALP and COL staining, and (C) ALP activity. Data are presented as the mean ± SD (n=3). *p<0.05

Additional file 3 of WNT16 elevation induced cell senescence of osteoblasts in ankylosing spondylitis

Additional file 3: Supplementary Figure 3. Analysis of WNT16 level in collected sera. The human WNT16 levels were measures in the collected sera (HC, n=3; AS, n=36) using ELISA. N.S., not significant

Additional file 1 of WNT16 elevation induced cell senescence of osteoblasts in ankylosing spondylitis

Additional file 1: Supplementary Figure 1. The overall image of a well for 96 well plates and its enlarged changes by Nikon microscopy

Additional file 2 of WNT16 elevation induced cell senescence of osteoblasts in ankylosing spondylitis

Additional file 2: Supplementary Figure 2. The result of siRNA efficiency test. (A) Total four human osteoblasts cell lines (MG63, FOB, SaOS2, U2OS) were extracted total RNA and analyzed by RT-PCR to test WNT16 level. (B) FOB cell were transfected with five siRNA oligo against human WNT16 and analyzed by RT-PCR for siRNA efficiency

Additional file 1: of IL-17A induces osteoblast differentiation by activating JAK2/STAT3 in ankylosing spondylitis

Figure S1. Effect of osteogenic differentiation on both Ct-BdCs and AS-BdCs. Figure S2. Correlation of IL-17 levels in AS patients with ESR, CRP, and BASDAI levels. Figure S3. Correlation of IL-17 levels in RA patients with ESR and CRP levels. Figure S4. Secukinumab inhibits AS serum-induced phosphorylation status of JAK2/STAT3 in Ct-BdCs. Figure S5. Secukinumab suppresses IL-17A dose-dependent ALP activation in Ct-BdCs. Figure S6. Effect of JAK2 inhibitor (AG490) treatment on viability and toxicity of both Ct- and AS-BdCs. Table S1. Primer Sequences for qPCR. Table S2. Primary antibodies used in Immunoblotting (IB), Immunostaining (IF), and Immunohistochemistry (IHC). (DOCX 826 kb

Additional file 1: of High level of interleukin-32 gamma in the joint of ankylosing spondylitis is associated with osteoblast differentiation

The protein levels of inflammatory cytokines including TNF-α, IL-1β, IL-18 and IL-22 were determined in the culture supernatant from the cells of WT or IL-32γ TG mice (A) and the cells in the absence (None) or presence of IL-32γ (B) after 1 week of OB differentiation using commercial available ELISA kits. The bars show the means ± SD of triplicate experiments. (TIFF 443 kb