The structures of nine identified compounds in <i>Pyropolyporus fomentarius</i> PE fraction.

<p>The structures of nine identified compounds in <i>Pyropolyporus fomentarius</i> PE fraction.</p

Tumors excised three weeks after treatment with <i>Pyropolyporus fomentarius</i> PE fraction from each mice group.

<p>(A) Image of tumors: (a) Control (CTL); (b) 120 mg/kg.d.w; (c) 240 mg/kg.d.w; (d) Cyclophosphamide (CTX), 20 mg/kg.d.w. (B) Determination of tumor weight, significant difference between control and treatment group was indicated by**p<0.01, values are mean±SD, n = 6.</p

Fabrication of (Calcein–ZnS)<i>_n</i> Ordered Ultrathin Films on the Basis of Layered Double Hydroxide and Its Ethanol Sensing Behavior

(Calcein–ZnS)n ultrathin films (UTFs) were fabricated through a two-step procedure including the layer-by-layer (LBL) assembly of calcein and exfoliated Zn2Al layered double hydroxide (LDH) nanosheets, followed by an in situ gas/solid reaction with H2S. The assembly process of calcein and exfoliated LDH was monitored by UV–vis absorption measurements to get a stepwise and regular growth of the (calcein–LDH)n UTFs upon increasing the deposited cycles. By an in situ gas–solid reaction with H2S, the resulting sulfurization derivatives denoted as (calcein–ZnS)n UTFs were obtained. The resulting (calcein–ZnS)30 UTF possesses high ethanol sensing performance (response value is 8.9–100 ppm ethanol) at relatively low working temperature (90 °C). The LBL assembly process and chemical conversion technique based on Zn2Al–LDH combine the functional organic molecules and inorganic semiconductor, which enables us to develop innovative composite materials with adjustable compositions for a broad range of applications

Apoptosis-inducing effect of <i>Pyropolyporus fomentarius</i> PE fraction on S180 cells as detected by Annexin V-FITC (AV)/PI method.

<p>(A) cells were analyzed at 36 h post-treatment by flow cytometry. Dot-plot graphs show viable cells (AV⁻/PI⁻), early apoptotic cells (AV⁺/PI⁻), late apoptotic cells(AV⁺/PI⁺), and necrotic cells (AV⁻/PI⁺). (B) The ratio of early apoptotic cells and late apoptotic cells are represented as means ±SD (n = 3). *p<0.05 and **p<0.01 versus the control (CTL).</p

Identification of <i>P</i>. <i>fomentarius</i> PE fraction metabolites using GC–MS analysis.

<p>Identification of <i>P</i>. <i>fomentarius</i> PE fraction metabolites using GC–MS analysis.</p

Organ index (mg/g BW) of repeated dose 3-weeks PFPE-treated mice^a.

<p>* p<0.05 vs model control group.</p>a<p>Data are expressed as means ± SD (n = 6).</p><p>Organ index (mg/g BW) of repeated dose 3-weeks PFPE-treated mice^{<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109599#nt102" target="_blank">a</a>}.</p

<i>Pyropolyporus fomentarius</i> PE fraction stimulated intracellular ROS generation in S180 cells.

<p>Cells were treated with different concentrations of PE fraction for 36 h (A), and treated at 480 µg/ml dose for various time periods (B), and labeled with DCFH–DA. The fluorescence intensity of the oxidized product DCF in individual cells was detected by flow cytometry. Each value is expressed as a mean ±SD of three independent experiments.**p<0.01 versus the control (CTL).</p

Effect of <i>Pyropolyporus fomentarius</i> PE fraction on the mitochondrial membrane potential of S180 cells.

<p>Cells were treated with different concentrations of PE fraction for 36 h (A), and treated at 480 µg/ml dose for various time periods (B), and stained with PI and labeled with rhodamine 123, analyzed by flow cytometry. Each value is expressed as a mean ±SD of three independent experiments.**p<0.01 versus the control (CTL).</p

Cytotoxicity effect of <i>Pyropolyporus fomentarius</i> PE fraction.

<p>(A) S180 cells were treated with 0, 120, 240 and 480 µg/ml of PE fraction for 24 h, 48 h. (B) Cell proliferation of PE fraction between HEK 293 cells and S180 cells at 48 h. Each value is expressed as a mean ±SD of three independent experiments. **p<0.01 versus the control (CTL).</p

DNA fragmentation assay of S180 cells exposed to <i>Pyropolyporus fomentarius</i> PE fraction.

<p>Cells were treated with different concentrations of PE fraction for 36 h (A), and treated at 480 µg/ml dose for various time periods (B), and stained with PI and analyzed by flow cytometry. Histograms show number of cell channel (vertical axis) vs. PI fluorescence (horizontal axis). Each value is expressed as a mean ±SD of three independent experiments. *p<0.05 and **p<0.01 versus the control (CTL).</p