DataSheet_1_Efficacy and safety of non-invasive brain stimulation in combination with antidepressants in adolescents with depression: a systematic review and meta-analysis.docx

ObjectiveNon-invasive brain stimulation (NIBS) is beneficial to adult patients with depression, but its safety and efficacy in combination with antidepressants in children and adolescents with depression are not clear. We conducted a preliminary meta-analysis to objectively evaluate its clinical effect and provide information for future research and clinical practice.MethodsPubMed, Cochrane Library, Embase, and Web of Science were searched systematically to find clinical trials published in English before April 11, 2023. Stata software was used for meta-analysis, and random or fixed effect models were used to combine effect sizes.ResultsNine studies were eligible and included (n = 393). No articles about children were included in the analysis. The results showed that the remission rate was 40% (95% confidence interval [CI]: 13% to 71%). The scores of Children’s Depression Rating Scale (CRDS) and Hamilton’s depression scale (HAMD) significantly decreased compared to baseline value (MD = -27.04, 95% CI: -30.95, -23.12 and MD = -12.78, 95% CI: -19.55 to -6.01). In addition, the incidence of all adverse events was 13% (95% CI: 5%, 23%), and all were minor pain-related events.ConclusionThe combination of NIBS and antidepressants has been shown to notably alleviate depressive symptoms in adolescents, offering a considerable level of safety. This therapeutic synergy is particularly effective in patients with major depressive disorder, where repetitive transcranial magnetic stimulation augmented with antidepressants can enhance the amelioration of depressive symptoms.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023442215, PROSPERO CRD42023442215.</p

DataSheet_2_Efficacy and safety of non-invasive brain stimulation in combination with antidepressants in adolescents with depression: a systematic review and meta-analysis.docx

ObjectiveNon-invasive brain stimulation (NIBS) is beneficial to adult patients with depression, but its safety and efficacy in combination with antidepressants in children and adolescents with depression are not clear. We conducted a preliminary meta-analysis to objectively evaluate its clinical effect and provide information for future research and clinical practice.MethodsPubMed, Cochrane Library, Embase, and Web of Science were searched systematically to find clinical trials published in English before April 11, 2023. Stata software was used for meta-analysis, and random or fixed effect models were used to combine effect sizes.ResultsNine studies were eligible and included (n = 393). No articles about children were included in the analysis. The results showed that the remission rate was 40% (95% confidence interval [CI]: 13% to 71%). The scores of Children’s Depression Rating Scale (CRDS) and Hamilton’s depression scale (HAMD) significantly decreased compared to baseline value (MD = -27.04, 95% CI: -30.95, -23.12 and MD = -12.78, 95% CI: -19.55 to -6.01). In addition, the incidence of all adverse events was 13% (95% CI: 5%, 23%), and all were minor pain-related events.ConclusionThe combination of NIBS and antidepressants has been shown to notably alleviate depressive symptoms in adolescents, offering a considerable level of safety. This therapeutic synergy is particularly effective in patients with major depressive disorder, where repetitive transcranial magnetic stimulation augmented with antidepressants can enhance the amelioration of depressive symptoms.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023442215, PROSPERO CRD42023442215.</p

Regulation of a Solid-Electrolyte Interphase and Ion Transfer via Organic Lewis Acid for Stable Li–S Pouch Cells

Li–S batteries have good application potential due to their high theoretical energy density/capacity, being widely studied in recent years. However, due to the high reactivity between Li and S and the low efficiency of ion transfer in electrolyte, side reactions and Li dendrite growth could easily happen and reduce the stability or the cycling life of Li–S batteries. In this work, a kind of Lewis acid, i.e., iodopentafluorobenzene (IPFB), is adopted as an electrolyte additive to regulate ion configurations and SEI formation. The Lewis acid property of IPFB could help adsorb anions in electrolyte, which could participate in SEI formation and weaken local space charge near the anode surface, hence realizing a uniform ion distribution and Li deposition. As a result, a long cycling stability of Li||Li cells for over 600 h at 3 mA cm–2 under 3 mAh cm–2 and a 40-cycle high stability at 0.4 C of Li–S pouch cells are achieved. The induction of Lewis acid into electrolytes could be a potential and facile strategy for realizing uniform Li deposition and high-performance Li–S pouch cells

Supplementary document for An intelligent scoring system based on dynamic optical breast imaging for early detection of breast cancer - 6815277.pdf

Supplementary Materia

Identification and Characterization of the 28‑<i>N</i>‑Methyltransferase Involved in HSAF Analogue Biosynthesis

Polycyclic tetramate macrolactams (PoTeMs) are a family of structurally intriguing bioactive natural products. Although the presence of the N-28 methyl group is known to affect bioactivities of some PoTeMs, the mechanism for this methylation remains unclear. We report here the identification and characterization of the 28-N-methyltransferase for HSAF analogues, which is encoded by a gene located outside the HSAF (heat-stable antifungal factor) cluster in Lysobacter enzymogenes C3. Our data suggested that 28-N-methyltransferase utilizes S-adenosylmethionine (SAM) to methylate HSAF analogues, and acts after the dicyclic and tricyclic ring formation and prior to C-3 hydroxylation. Kinetic analysis showed that the optimal substrate for the enzyme is 3-dehydroxy HSAF (3-deOH HSAF). Moreover, it could also accept PoTeMs bearing a 5–6 or 5–6–5 polycyclic system as substrates. This is the first N-methyltransferase identified in the family of PoTeMs, and the identification of this enzyme provides a new tool to generate new PoTeMs as antibiotic lead compounds

Hygrocins C–G, Cytotoxic Naphthoquinone Ansamycins from <i>gdmAI</i>-Disrupted <i>Streptomyces</i> sp. LZ35

Six hygrocins, polyketides of ansamycin class, were isolated from the <i>gdmAI</i>-disrupted Streptomyces sp. LZ35. The planar structure of hygrocins C–E (<b>1</b>–<b>3</b>) was determined by one-dimensional and two-dimensional NMR spectroscopy and high-resolution mass spectrometry. They are derivatives of hygrocin A but differ in the configuration at C-2 and the orientation of the C-3,4 double bond. Hygrocin F­(<b>4</b>) and G­(<b>5</b>) were shown to be isomers of hygrocin C (<b>1</b>) and B (<b>6</b>), respectively, due to the different alkyl oxygen participating in the macrolide ester linkage. Hygrocins C, D, and F were found to be toxic to human breast cancer MDA-MB-431 cells (IC₅₀ = 0.5, 3.0, and 3.3 μM, respectively) and prostate cancer PC3 cells (IC₅₀ = 1.9, 5.0, and 4.5 μM, respectively), while hygrocins B, E, and G were inactive

Activating a Cryptic Ansamycin Biosynthetic Gene Cluster To Produce Three New Naphthalenic Octaketide Ansamycins with <i>n</i>‑Pentyl and <i>n</i>‑Butyl Side Chains

Genome mining is a rational approach to discovering new natural products. The genome sequence analysis of <i>Streptomyces</i> sp. LZ35 revealed the presence of a putative ansamycin gene cluster (<i>nam</i>). Constitutive overexpression of the pathway-specific transcriptional regulatory gene <i>nam1</i> successfully activated the <i>nam</i> gene cluster, and three novel naphthalenic octaketide ansamycins were discovered with unprecedented <i>n</i>-pentylmalonyl-CoA or <i>n</i>-butylmalonyl-CoA extender units. This study represents the first example of discovering novel ansamycin scaffolds via activation of a cryptic gene cluster

Unusual Activities of the Thioesterase Domain for the Biosynthesis of the Polycyclic Tetramate Macrolactam HSAF in <i>Lysobacter enzymogenes</i> C3

HSAF is an antifungal natural product with a new mode of action. A rare bacterial iterative PKS-NRPS assembles the HSAF skeleton. The biochemical characterization of the NRPS revealed that the thioesterase (TE) domain possesses the activities of both a protease and a peptide ligase. Active site mutagenesis, circular dichroism spectra, and homology modeling of the TE structure suggested that the TE may possess uncommon features that may lead to the unusual activities. The iterative PKS-NRPS is found in all polycyclic tetramate macrolactam gene clusters, and the unusual activities of the TE may be common to this type of hybrid PKS-NRPS

Hydrothermal Stability of Core–Shell Pd@Ce_0.5Zr_0.5O₂/Al₂O₃ Catalyst for Automobile Three-Way Reaction

In this paper, the hydrothermal stability and catalytic activity of Pd@Ce_0.5Zr_0.5O₂/Al₂O₃ catalyst with a core–shell structure were investigated for automobile three-way reactions and compared with those of Pd/Al₂O₃, Pd@CeO₂/Al₂O₃, and Pd@ZrO₂/Al₂O₃ catalysts. TEM, HRTEM, and EDS mapping analyses showed that the core–shell structure of Pd@Ce_0.5Zr_0.5O₂ nanoparticles was intact after the hydrothermal treatment at 1050 °C for 5 h. Meanwhile, CO–DRIFT results suggested that the interface of Pd core and Ce_0.5Zr_0.5O₂ shell acted as the active sites in the reaction of three-way catalysts. Additionally, XPS, FT-IR, and CO–DRIFT analyses demonstrated that a large amount of OH groups were present on the surface of Pd@Ce_0.5Zr_0.5O₂/Al₂O₃ catalyst, which could accelerate the decomposition of carbonate species and reduce the activation energy of the catalytic reaction. This was an important reason for the Pd@Ce_0.5Zr_0.5O₂/Al₂O₃ catalyst to keep the high catalytic activity after aging at high temperature

Targeted Discovery and Combinatorial Biosynthesis of Polycyclic Tetramate Macrolactam Combamides A–E

Polycyclic tetramate macrolactams (PoTeMs) are a growing class of natural products with distinct structure and diverse biological activities. By promoter engineering and heterologous expression of the cryptic <i>cbm</i> gene cluster, four new PoTeMs, combamides A–E (<b>1</b>–<b>4</b>), were identified. Additionally, two new derivatives, combamides E (<b>5</b>) and F (<b>6</b>), were generated via combinatorial biosynthesis. Together, our findings provide a sound base for expanding the structure diversities of PoTeMs through genome mining and combinatorial biosynthesis