107 research outputs found
Mdm2 C305F mutation decreases proliferation but does not affect apoptosis of <i>APT<sub>121</sub></i>-induced prostate cancer.
<p>A. Representative Ki67 staining of prostate sections from 6 month-old mice of the indicated genotypes. Brown staining indicates proliferating cells. Scale bar was shown in the first picture and all pictures were taken at the same magnification. B. Average%Ki67-positive cells ± SD from 6 month-old mice of the indicated genotypes. At least five independent fields consisting of a total of at least 1,000 cells from each prostate sample were counted. **p<0.01 as assessed by Student's t test. C. Representative TUNEL staining of prostate sections from 6 month-old mice of the indicated genotypes. Brown staining indicates apoptotic cells. Scale bar was shown in the first picture and all pictures were taken at the same magnification. D. Average%TUNEL-positive cells ± SD from 6 month-old mice of the indicated genotypes. At least five independent fields consisting of a total of at least 1,000 cells from each prostate sample were counted. (A–D) <i>Mdm2<sup>+/+</sup></i> (n = 8), <i>Mdm2<sup>C305F/C305F</sup></i> (n = 7), <i>APT<sub>121</sub>;Mdm2<sup>+/+</sup></i> (n = 7), and <i>APT<sub>121</sub>;Mdm2<sup>C305F/C305F</sup></i> (n = 9) mice were used.</p
Functional classification of different proteins.
<p>Note: The protein levels in DSM 1731 and 1731(pIMP1) under normal condition were used as the basis for comparison, respectively. The upregulation of fatty acid synthesis enzymes (acyl-ACP (acyl-carrier-protein) thioesterase and 3-oxoacyl-ACP synthase I) is more likely to be an indicator reflecting cell damage from butanol stress. The upregulation of HSPs is regarded as a common response to butanol stress, but not related with the function of SMB_G1518-1519.</p
Growth profiles for 1731(pIMP1), 1731(p1518-1519) and DDC14(p1518-1519)
<p>. A) Growth profiles under normal condition. B) Growth profiles under 1% butanol stress.</p
Mdm2 C305F mutation causes reduced prostate size and slows the progression of <i>APT<sub>121</sub></i>-induced prostate cancer.
<p>A. Photographs showing representative prostates from 6 month-old mice of the indicated genotypes. B. Average prostate mass ± SD from 6 month-old mice of the indicated genotypes. <i>Mdm2<sup>+/+</sup></i> (n = 12), <i>Mdm2<sup>C305F/C305F</sup></i> (n = 11), <i>APT<sub>121</sub>;Mdm2<sup>+/+</sup></i> (n = 9), and <i>APT<sub>121</sub>;Mdm2<sup>C305F/C305F</sup></i> (n = 13) . * p<0.05 and ** p<0.01 as assessed by Student's t test. C. Representative H&E staining of prostate sections from 6 month-old mice of the indicated genotypes demonstrating histology associated with the indicated stages of tumor progression. Scale bar was shown in the first picture and all pictures were taken at the same magnification.</p
Growth profiles for DSM 1731 and its deletion or disruption mutants.
<p>A) Growth profiles under normal condition. B) Growth profiles under 1% butanol stress.</p
Effects of p19Arf loss on tumor progression in <i>APT<sub>121</sub></i>-induced prostate cancer.
<p>A. Representative H&E staining of prostate sections from 5 month-old mice of the indicated genotypes. Stromal tumor was detected only in <i>APT<sub>121</sub></i>;p19Arf<sup>−/−</sup> mice as indicated by asterisk. Scale bar was shown in the first picture and all pictures were taken at the same magnification. B. Representative Ki67 staining from 5 month-old mice of the indicated genotypes. Scale bar was shown in the first picture and all pictures were taken at the same magnification. C. Average%Ki67-positive cells ± SD from 5 month-old mice of the indicated genotypes. n = 6 for each genotype. At least five independent fields consisting of a total of at least 1,000 cells from each prostate sample were counted. Brown staining indicates proliferating cells. *p = 0.01 as assessed by Student's t test. D. Representative TUNEL staining from 5 month-old mice of the indicated genotypes. Scale bar was shown in the first picture and all pictures were taken at the same magnification. E. Average%TUNEL-positive cells ± SD from 5 month-old mice of the indicated genotypes. n = 6 for each genotype. At least five independent fields consisting of a total of at least 1,000 cells from each prostate sample were counted. Brown staining indicates apoptotic cells.</p
Activated Ras induces a normal p53 response but does not up-regulate ribosomal protein L11.
<p>A. Detection of L11 and β-actin by immunoblot analysis of total cellular lysate prepared from the indicated mouse karatinocyte cell lines. β-actin serves as a loading control. B. Representative phase-contrast images of <i>Mdm2<sup>+/+</sup></i> and <i>Mdm2<sup>C305F/C305F</sup></i> MEFs stably infected with empty vector or H-Ras<sup>G12V</sup> retroviruses. C. Detection of L11, L5, p53, H-Ras, and β-actin by immunoblot analysis of total cellular lysate prepared from the MEFs described in B. β-Actin serves as a loading control.</p
The analysis of main fermentation products after 50% of growth inhibition degree was achieved.
<p>A) The analysis of main fermentation products after DSM 1731 and its deletion mutant DDC14 were treated by butanol for 6 h. B) The analysis of main fermentation products after the plasmid control strain 1731(pIMP1) and overexpression strain 1731(p1518-1519) were treated by butanol for 18 h.</p
Bacterial strains, plasmids and primers.
<p><b>Abbreviations</b>: Amp<sup>r</sup>, ampicillin resistance; Cm<sup>r</sup>, chloramphenicol resistance; Tet<sup>r</sup>, tetracycline resistance; <i>Φ3t1</i>, Φ3t1 methyltransferase gene of <i>Bacillus subtilis</i> phage Φ3t1. DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.</p
Overexpression of SMB_G1518-1519 influenced the motilities of <i>C. acetobutylicum</i>.
<p>Overexpression of SMB_G1518-1519 influenced the motilities of <i>C. acetobutylicum</i>.</p
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